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Genetic variation in several populations of Biawan fish (Helostoma temminckii) from Bakong river using DNA sequence & PCR-RFLP of cytochrome oxidase I (COI) mtDNA gene

Introduced aquatic species are defined as a plant or animal moved from one place to another by humans. Helostoma temminckii (C.&V.) was introduced into Sarawak in 1956 has invaded fivers and lakes of the lower Baram. To study the genetic variation of the fish, samples were collected from four...

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Main Author: Roslina, Kadri
Format: Final Year Project Report
Language:English
Published: Universiti Malaysia Sarawak, (UNIMAS) 2008
Subjects:
Q Science (General)
Online Access:http://ir.unimas.my/id/eprint/47606/1/Roslina%20Binti%20Kadri%20FT.pdf
http://ir.unimas.my/id/eprint/47606/
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spelling my.unimas.ir-476062025-02-17T07:43:07Z http://ir.unimas.my/id/eprint/47606/ Genetic variation in several populations of Biawan fish (Helostoma temminckii) from Bakong river using DNA sequence & PCR-RFLP of cytochrome oxidase I (COI) mtDNA gene Roslina, Kadri Q Science (General) Introduced aquatic species are defined as a plant or animal moved from one place to another by humans. Helostoma temminckii (C.&V.) was introduced into Sarawak in 1956 has invaded fivers and lakes of the lower Baram. To study the genetic variation of the fish, samples were collected from four sites along the Bakong River. Cytochrome oxidase I (COl) mtDNA gene was amplified by PCR and screened for genetic variation using two molecular methods. DNA sequencing and RFLP. Phylogenetic analysis was done using three methods, namely the distance matrix, maximum parsimony and maximum likelihood. The nucleotide divergence between samples varied between 1.0 and 4.0 %. There is an occurrence or gene flow among the populations but the genetic variation within all population is still low. The genetic variation was low in all populations probably because they have only been introduced recently, about 50 years ago. Universiti Malaysia Sarawak, (UNIMAS) 2008 Final Year Project Report NonPeerReviewed text en http://ir.unimas.my/id/eprint/47606/1/Roslina%20Binti%20Kadri%20FT.pdf Roslina, Kadri (2008) Genetic variation in several populations of Biawan fish (Helostoma temminckii) from Bakong river using DNA sequence & PCR-RFLP of cytochrome oxidase I (COI) mtDNA gene. [Final Year Project Report] (Unpublished)
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
topic Q Science (General)
spellingShingle Q Science (General)
Roslina, Kadri
Genetic variation in several populations of Biawan fish (Helostoma temminckii) from Bakong river using DNA sequence & PCR-RFLP of cytochrome oxidase I (COI) mtDNA gene
description Introduced aquatic species are defined as a plant or animal moved from one place to another by humans. Helostoma temminckii (C.&V.) was introduced into Sarawak in 1956 has invaded fivers and lakes of the lower Baram. To study the genetic variation of the fish, samples were collected from four sites along the Bakong River. Cytochrome oxidase I (COl) mtDNA gene was amplified by PCR and screened for genetic variation using two molecular methods. DNA sequencing and RFLP. Phylogenetic analysis was done using three methods, namely the distance matrix, maximum parsimony and maximum likelihood. The nucleotide divergence between samples varied between 1.0 and 4.0 %. There is an occurrence or gene flow among the populations but the genetic variation within all population is still low. The genetic variation was low in all populations probably because they have only been introduced recently, about 50 years ago.
format Final Year Project Report
author Roslina, Kadri
author_facet Roslina, Kadri
author_sort Roslina, Kadri
title Genetic variation in several populations of Biawan fish (Helostoma temminckii) from Bakong river using DNA sequence & PCR-RFLP of cytochrome oxidase I (COI) mtDNA gene
title_short Genetic variation in several populations of Biawan fish (Helostoma temminckii) from Bakong river using DNA sequence & PCR-RFLP of cytochrome oxidase I (COI) mtDNA gene
title_full Genetic variation in several populations of Biawan fish (Helostoma temminckii) from Bakong river using DNA sequence & PCR-RFLP of cytochrome oxidase I (COI) mtDNA gene
title_fullStr Genetic variation in several populations of Biawan fish (Helostoma temminckii) from Bakong river using DNA sequence & PCR-RFLP of cytochrome oxidase I (COI) mtDNA gene
title_full_unstemmed Genetic variation in several populations of Biawan fish (Helostoma temminckii) from Bakong river using DNA sequence & PCR-RFLP of cytochrome oxidase I (COI) mtDNA gene
title_sort genetic variation in several populations of biawan fish (helostoma temminckii) from bakong river using dna sequence & pcr-rflp of cytochrome oxidase i (coi) mtdna gene
publisher Universiti Malaysia Sarawak, (UNIMAS)
publishDate 2008
url http://ir.unimas.my/id/eprint/47606/1/Roslina%20Binti%20Kadri%20FT.pdf
http://ir.unimas.my/id/eprint/47606/
_version_ 1825166850783182848
score 13.244413

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