Cover Image

Isolation and characterization of a thermostable cellulase from thermophilic microorganisms obtained Anah Rais hot spring

this study concerned about the isolation and identification of thermophilic microorganisms that has the thermostable enzyme obtained frum Anah Rais Hot Spring. On minimal media containing CMC, five colonies of microorganisms have been isolated and identified as AR1 Bacillus spp., AR2 Nisseria spp...

Full description

Saved in:
Bibliographic Details
Main Author: Noor Izzati, Yacob
Format: Final Year Project Report
Language:English
Published: Universiti Malaysia Sarawak, (UNIMAS) 2005
Subjects:
Q Science (General)
Online Access:http://ir.unimas.my/id/eprint/47596/1/Noor%20lzzati%20Binti%20Yacob%20FT.pdf
http://ir.unimas.my/id/eprint/47596/
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.unimas.ir-47596
record_format eprints
spelling my.unimas.ir-475962025-02-17T05:54:16Z http://ir.unimas.my/id/eprint/47596/ Isolation and characterization of a thermostable cellulase from thermophilic microorganisms obtained Anah Rais hot spring Noor Izzati, Yacob Q Science (General) this study concerned about the isolation and identification of thermophilic microorganisms that has the thermostable enzyme obtained frum Anah Rais Hot Spring. On minimal media containing CMC, five colonies of microorganisms have been isolated and identified as AR1 Bacillus spp., AR2 Nisseria spp., AR3 Enterobacter spp., AK4 Streptococcus spp., and AR5 Klebsiella spp. Cellulase enzyme activity was indicated by enzyme degradation of the CMC media that produced clear haloes around the supernatant (containing the enzyme) of each microorganism after flooded with Congo red. Later, the microorganisms were grown on fermentation media consists of O.5% w/v sago and paddy pith waste. 0.7% w/v yeast extract, 0.1% w/v KH2PO4 and 0.5% w/v MgSO. The enzyme activity was determined using DNS and FPase enzyme assay. Bradford assay was used to determine the protein content. Isolate show the highest cellulolytic activity was preceded to enzyme purification that comprised ((NH4)2SO4) precipitation and HIC. SDS-PAGE was used to determine the purity and molecular weight of cellulase enzyme. Genomic DNA from each microorganism were extracted and used for molecular identification. Through enzyme assay, isolate of AR4 Streptococcus spp was characterized as the best thermophilic microorganisms. The crude enzymec of the isolate was found to show highest enzyme activity (Sago/paddy pith waste media of 0.428U/ml) within 15 hour at temperature 55°C during enzyme substrate reaction. However, gene encoding cellulase enzyme from this isolate could not obtain. Universiti Malaysia Sarawak, (UNIMAS) 2005 Final Year Project Report NonPeerReviewed text en http://ir.unimas.my/id/eprint/47596/1/Noor%20lzzati%20Binti%20Yacob%20FT.pdf Noor Izzati, Yacob (2005) Isolation and characterization of a thermostable cellulase from thermophilic microorganisms obtained Anah Rais hot spring. [Final Year Project Report] (Unpublished)
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
topic Q Science (General)
spellingShingle Q Science (General)
Noor Izzati, Yacob
Isolation and characterization of a thermostable cellulase from thermophilic microorganisms obtained Anah Rais hot spring
description this study concerned about the isolation and identification of thermophilic microorganisms that has the thermostable enzyme obtained frum Anah Rais Hot Spring. On minimal media containing CMC, five colonies of microorganisms have been isolated and identified as AR1 Bacillus spp., AR2 Nisseria spp., AR3 Enterobacter spp., AK4 Streptococcus spp., and AR5 Klebsiella spp. Cellulase enzyme activity was indicated by enzyme degradation of the CMC media that produced clear haloes around the supernatant (containing the enzyme) of each microorganism after flooded with Congo red. Later, the microorganisms were grown on fermentation media consists of O.5% w/v sago and paddy pith waste. 0.7% w/v yeast extract, 0.1% w/v KH2PO4 and 0.5% w/v MgSO. The enzyme activity was determined using DNS and FPase enzyme assay. Bradford assay was used to determine the protein content. Isolate show the highest cellulolytic activity was preceded to enzyme purification that comprised ((NH4)2SO4) precipitation and HIC. SDS-PAGE was used to determine the purity and molecular weight of cellulase enzyme. Genomic DNA from each microorganism were extracted and used for molecular identification. Through enzyme assay, isolate of AR4 Streptococcus spp was characterized as the best thermophilic microorganisms. The crude enzymec of the isolate was found to show highest enzyme activity (Sago/paddy pith waste media of 0.428U/ml) within 15 hour at temperature 55°C during enzyme substrate reaction. However, gene encoding cellulase enzyme from this isolate could not obtain.
format Final Year Project Report
author Noor Izzati, Yacob
author_facet Noor Izzati, Yacob
author_sort Noor Izzati, Yacob
title Isolation and characterization of a thermostable cellulase from thermophilic microorganisms obtained Anah Rais hot spring
title_short Isolation and characterization of a thermostable cellulase from thermophilic microorganisms obtained Anah Rais hot spring
title_full Isolation and characterization of a thermostable cellulase from thermophilic microorganisms obtained Anah Rais hot spring
title_fullStr Isolation and characterization of a thermostable cellulase from thermophilic microorganisms obtained Anah Rais hot spring
title_full_unstemmed Isolation and characterization of a thermostable cellulase from thermophilic microorganisms obtained Anah Rais hot spring
title_sort isolation and characterization of a thermostable cellulase from thermophilic microorganisms obtained anah rais hot spring
publisher Universiti Malaysia Sarawak, (UNIMAS)
publishDate 2005
url http://ir.unimas.my/id/eprint/47596/1/Noor%20lzzati%20Binti%20Yacob%20FT.pdf
http://ir.unimas.my/id/eprint/47596/
_version_ 1825166848971243520
score 13.244413

Similar Items