Staff View: Amplicon Sequencing Reveals Bacterial Diversity of Indoor Air Microbiome in Hospital Buildings

Amplicon Sequencing Reveals Bacterial Diversity of Indoor Air Microbiome in Hospital Buildings

Airborne transmission of pathogens in hospitals poses a significant risk for healthcare-associated infections. Traditionally, assessing microorganisms in hospital air relies on culture methods,limiting the identification process. Advances in genome sequencing technology now allow for more accurate...

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Main Authors:	Nor Husna, Mat Hussin, Junaidah, Zakaria, Mohd Norhafsam, Maghpor, Ummu Afeera, Zainul Abid, Shamrulazhar, Shamzir Kamal, Hajar Fauzan, Ahmad
Format:	Article
Language:	English
Published:	National Institute of Occupational Safety and Health (NIOSH) 2024
Subjects:	Q Science (General) QR Microbiology QR180 Immunology
Online Access:	http://ir.unimas.my/id/eprint/47164/3/Amplicon%20Sequencing%20Reveals%20Bacterial%20-%20Copy.pdf http://ir.unimas.my/id/eprint/47164/ http://www.niosh.com.my/publication/niosh-journal
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id	my.unimas.ir-47164
record_format	eprints
spelling	my.unimas.ir-471642025-01-02T00:50:20Z http://ir.unimas.my/id/eprint/47164/ Amplicon Sequencing Reveals Bacterial Diversity of Indoor Air Microbiome in Hospital Buildings Nor Husna, Mat Hussin Junaidah, Zakaria Mohd Norhafsam, Maghpor Ummu Afeera, Zainul Abid Shamrulazhar, Shamzir Kamal Hajar Fauzan, Ahmad Q Science (General) QR Microbiology QR180 Immunology Airborne transmission of pathogens in hospitals poses a significant risk for healthcare-associated infections. Traditionally, assessing microorganisms in hospital air relies on culture methods,limiting the identification process. Advances in genome sequencing technology now allow for more accurate and unbiased identification of microbial content. This study employed amplicon sequencing to analyse the bacterial community in the indoor air microbiome of Sultan Ahmad Shah Medical Centre (SASMEC). Dust samples from 12 randomly selected airhandling unit (AHU) rooms were collected, and genomic DNA was extracted and sequenced targeting the V3 region of 16S rRNA. The Illumina NovaSeq 6000 system performed short-read amplicon sequencing. The results revealed diverse bacterial communities in AHU supply and return units, with Proteobacteria, Firmicutes, and Actinobacteria being the most common phyla. Dominant bacterial species included Methylobacterium spp., Nesterenkonia spp., Rubrobacter A bracarensis, Flavobacteriaceae spp., and Salinisphaera spp., with Methylobacterium spp. posing concerns due to its association with opportunistic infections. The study highlights the importance of using next-generation sequencing and culture-independent methods to monitor indoor air microbiomes. This provides crucial insights for managing biological contaminants, including airborne transmission, and enhances infectious disease surveillance in healthcare settings. National Institute of Occupational Safety and Health (NIOSH) 2024 Article PeerReviewed text en http://ir.unimas.my/id/eprint/47164/3/Amplicon%20Sequencing%20Reveals%20Bacterial%20-%20Copy.pdf Nor Husna, Mat Hussin and Junaidah, Zakaria and Mohd Norhafsam, Maghpor and Ummu Afeera, Zainul Abid and Shamrulazhar, Shamzir Kamal and Hajar Fauzan, Ahmad (2024) Amplicon Sequencing Reveals Bacterial Diversity of Indoor Air Microbiome in Hospital Buildings. Journal of Occupational Safety and Health, 21 (1). pp. 1-10. ISSN 1675-5456 http://www.niosh.com.my/publication/niosh-journal
institution	Universiti Malaysia Sarawak
building	Centre for Academic Information Services (CAIS)
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Malaysia Sarawak
content_source	UNIMAS Institutional Repository
url_provider	http://ir.unimas.my/
language	English
topic	Q Science (General) QR Microbiology QR180 Immunology
spellingShingle	Q Science (General) QR Microbiology QR180 Immunology Nor Husna, Mat Hussin Junaidah, Zakaria Mohd Norhafsam, Maghpor Ummu Afeera, Zainul Abid Shamrulazhar, Shamzir Kamal Hajar Fauzan, Ahmad Amplicon Sequencing Reveals Bacterial Diversity of Indoor Air Microbiome in Hospital Buildings
description	Airborne transmission of pathogens in hospitals poses a significant risk for healthcare-associated infections. Traditionally, assessing microorganisms in hospital air relies on culture methods,limiting the identification process. Advances in genome sequencing technology now allow for more accurate and unbiased identification of microbial content. This study employed amplicon sequencing to analyse the bacterial community in the indoor air microbiome of Sultan Ahmad Shah Medical Centre (SASMEC). Dust samples from 12 randomly selected airhandling unit (AHU) rooms were collected, and genomic DNA was extracted and sequenced targeting the V3 region of 16S rRNA. The Illumina NovaSeq 6000 system performed short-read amplicon sequencing. The results revealed diverse bacterial communities in AHU supply and return units, with Proteobacteria, Firmicutes, and Actinobacteria being the most common phyla. Dominant bacterial species included Methylobacterium spp., Nesterenkonia spp., Rubrobacter A bracarensis, Flavobacteriaceae spp., and Salinisphaera spp., with Methylobacterium spp. posing concerns due to its association with opportunistic infections. The study highlights the importance of using next-generation sequencing and culture-independent methods to monitor indoor air microbiomes. This provides crucial insights for managing biological contaminants, including airborne transmission, and enhances infectious disease surveillance in healthcare settings.
format	Article
author	Nor Husna, Mat Hussin Junaidah, Zakaria Mohd Norhafsam, Maghpor Ummu Afeera, Zainul Abid Shamrulazhar, Shamzir Kamal Hajar Fauzan, Ahmad
author_facet	Nor Husna, Mat Hussin Junaidah, Zakaria Mohd Norhafsam, Maghpor Ummu Afeera, Zainul Abid Shamrulazhar, Shamzir Kamal Hajar Fauzan, Ahmad
author_sort	Nor Husna, Mat Hussin
title	Amplicon Sequencing Reveals Bacterial Diversity of Indoor Air Microbiome in Hospital Buildings
title_short	Amplicon Sequencing Reveals Bacterial Diversity of Indoor Air Microbiome in Hospital Buildings
title_full	Amplicon Sequencing Reveals Bacterial Diversity of Indoor Air Microbiome in Hospital Buildings
title_fullStr	Amplicon Sequencing Reveals Bacterial Diversity of Indoor Air Microbiome in Hospital Buildings
title_full_unstemmed	Amplicon Sequencing Reveals Bacterial Diversity of Indoor Air Microbiome in Hospital Buildings
title_sort	amplicon sequencing reveals bacterial diversity of indoor air microbiome in hospital buildings
publisher	National Institute of Occupational Safety and Health (NIOSH)
publishDate	2024
url	http://ir.unimas.my/id/eprint/47164/3/Amplicon%20Sequencing%20Reveals%20Bacterial%20-%20Copy.pdf http://ir.unimas.my/id/eprint/47164/ http://www.niosh.com.my/publication/niosh-journal
_version_	1821007917570064384
score	13.235796

Amplicon Sequencing Reveals Bacterial Diversity of Indoor Air Microbiome in Hospital Buildings

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