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Morphology and Molecular Characterisation of Karenia mikimotoi (Dinophyceae) from Sabah Malaysian Borneo, with a Focus on the Second Internal Transcribed Spacer (ITS2) of Ribosomal RNA gene

The first recorded bloom of Karenia mikimotoi (initially Gymnodinium mikimotoi) occurred off the coast of Japan in 1934, causing mass mortality of shellfish and fish. This event highlighted the devastating impact of K. mikimotoi blooms and marked a turning point in harmful algal bloom (HAB) resear...

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Main Authors: SHERYL UNCHA, ANDREW CHIBA, SING TUNG, TENG, SAMSUR, MOHAMAD, NURSYAHIDA, ABDULLAH, ING KUO, LAW, PO TEEN, LIM, CHUI PIN, LEAW
Format: Article
Language:English
Published: UNIMAS Publisher 2024
Subjects:
QH301 Biology
Online Access:http://ir.unimas.my/id/eprint/47109/1/7.%2B%2881-97%29%2BBJRST-590%20%281%29.pdf
http://ir.unimas.my/id/eprint/47109/
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https://doi.org/10.33736/bjrst.7417.2024
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spelling my.unimas.ir-471092024-12-30T08:21:47Z http://ir.unimas.my/id/eprint/47109/ Morphology and Molecular Characterisation of Karenia mikimotoi (Dinophyceae) from Sabah Malaysian Borneo, with a Focus on the Second Internal Transcribed Spacer (ITS2) of Ribosomal RNA gene SHERYL UNCHA, ANDREW CHIBA SING TUNG, TENG SAMSUR, MOHAMAD NURSYAHIDA, ABDULLAH ING KUO, LAW PO TEEN, LIM CHUI PIN, LEAW QH301 Biology The first recorded bloom of Karenia mikimotoi (initially Gymnodinium mikimotoi) occurred off the coast of Japan in 1934, causing mass mortality of shellfish and fish. This event highlighted the devastating impact of K. mikimotoi blooms and marked a turning point in harmful algal bloom (HAB) research, driving studies on its identification, biology, toxicology, and effects on marine life and ecosystems. The past reported bloom events in Southeast Asia have raised public concerns, leading to further investigation into the occurrence and geographical distribution of K. mikimotoi in the region. As of yet, there is no recorded evidence of K. mikimotoi blooms in Malaysian waters. This prompt the investigation of the occurrence and distribution of K. mikimotoi in Malaysia, and this study represent the first record of K. mikimotoi in Malaysian waters. In this study, clonal cultures of K. mikimotoi isolated from Sepanggar Bay, Sabah were examined using light microscopy (LM) and scanning electron microscopy (SEM) to observe its morphological features. Cells of K. mikimotoi from Malaysian Borneo exhibited a typical dorso-ventrally flattened body with bi-lobed and linear apical grooves on the cell apex. Molecular characterisation of the strains based on the internal transcribed spacer (ITS) region and large-subunit (LSU) ribosomal DNA revealed close phylogenetic relationships with other strains of K. mikimotoi from other regions, forming a monophyletic clade that positioned as sister to K. brevis, supporting the species identity of K. mikimotoi. The secondary structure of the ITS2 RNA transcript revealed a universal structure with four major helices. Structural comparison between K. mikimotoi and its relatives revealed four to six hemi-compensatory base changes. The results demonstrated the efficacy of ITS2 secondary structure information in delimiting species in Karenia. The detailed morphology and molecular characteristics of K. mikimotoi were revealed, for the first time, from the coastal waters of Malaysian Borneo. UNIMAS Publisher 2024-12-23 Article PeerReviewed text en http://ir.unimas.my/id/eprint/47109/1/7.%2B%2881-97%29%2BBJRST-590%20%281%29.pdf SHERYL UNCHA, ANDREW CHIBA and SING TUNG, TENG and SAMSUR, MOHAMAD and NURSYAHIDA, ABDULLAH and ING KUO, LAW and PO TEEN, LIM and CHUI PIN, LEAW (2024) Morphology and Molecular Characterisation of Karenia mikimotoi (Dinophyceae) from Sabah Malaysian Borneo, with a Focus on the Second Internal Transcribed Spacer (ITS2) of Ribosomal RNA gene. Borneo Journal of Resource Science and Technology, 14 (2). pp. 81-97. ISSN 2229-9769 https://publisher.unimas.my/ojs/index.php/BJRST/about https://doi.org/10.33736/bjrst.7417.2024
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
topic QH301 Biology
spellingShingle QH301 Biology
SHERYL UNCHA, ANDREW CHIBA
SING TUNG, TENG
SAMSUR, MOHAMAD
NURSYAHIDA, ABDULLAH
ING KUO, LAW
PO TEEN, LIM
CHUI PIN, LEAW
Morphology and Molecular Characterisation of Karenia mikimotoi (Dinophyceae) from Sabah Malaysian Borneo, with a Focus on the Second Internal Transcribed Spacer (ITS2) of Ribosomal RNA gene
description The first recorded bloom of Karenia mikimotoi (initially Gymnodinium mikimotoi) occurred off the coast of Japan in 1934, causing mass mortality of shellfish and fish. This event highlighted the devastating impact of K. mikimotoi blooms and marked a turning point in harmful algal bloom (HAB) research, driving studies on its identification, biology, toxicology, and effects on marine life and ecosystems. The past reported bloom events in Southeast Asia have raised public concerns, leading to further investigation into the occurrence and geographical distribution of K. mikimotoi in the region. As of yet, there is no recorded evidence of K. mikimotoi blooms in Malaysian waters. This prompt the investigation of the occurrence and distribution of K. mikimotoi in Malaysia, and this study represent the first record of K. mikimotoi in Malaysian waters. In this study, clonal cultures of K. mikimotoi isolated from Sepanggar Bay, Sabah were examined using light microscopy (LM) and scanning electron microscopy (SEM) to observe its morphological features. Cells of K. mikimotoi from Malaysian Borneo exhibited a typical dorso-ventrally flattened body with bi-lobed and linear apical grooves on the cell apex. Molecular characterisation of the strains based on the internal transcribed spacer (ITS) region and large-subunit (LSU) ribosomal DNA revealed close phylogenetic relationships with other strains of K. mikimotoi from other regions, forming a monophyletic clade that positioned as sister to K. brevis, supporting the species identity of K. mikimotoi. The secondary structure of the ITS2 RNA transcript revealed a universal structure with four major helices. Structural comparison between K. mikimotoi and its relatives revealed four to six hemi-compensatory base changes. The results demonstrated the efficacy of ITS2 secondary structure information in delimiting species in Karenia. The detailed morphology and molecular characteristics of K. mikimotoi were revealed, for the first time, from the coastal waters of Malaysian Borneo.
format Article
author SHERYL UNCHA, ANDREW CHIBA
SING TUNG, TENG
SAMSUR, MOHAMAD
NURSYAHIDA, ABDULLAH
ING KUO, LAW
PO TEEN, LIM
CHUI PIN, LEAW
author_facet SHERYL UNCHA, ANDREW CHIBA
SING TUNG, TENG
SAMSUR, MOHAMAD
NURSYAHIDA, ABDULLAH
ING KUO, LAW
PO TEEN, LIM
CHUI PIN, LEAW
author_sort SHERYL UNCHA, ANDREW CHIBA
title Morphology and Molecular Characterisation of Karenia mikimotoi (Dinophyceae) from Sabah Malaysian Borneo, with a Focus on the Second Internal Transcribed Spacer (ITS2) of Ribosomal RNA gene
title_short Morphology and Molecular Characterisation of Karenia mikimotoi (Dinophyceae) from Sabah Malaysian Borneo, with a Focus on the Second Internal Transcribed Spacer (ITS2) of Ribosomal RNA gene
title_full Morphology and Molecular Characterisation of Karenia mikimotoi (Dinophyceae) from Sabah Malaysian Borneo, with a Focus on the Second Internal Transcribed Spacer (ITS2) of Ribosomal RNA gene
title_fullStr Morphology and Molecular Characterisation of Karenia mikimotoi (Dinophyceae) from Sabah Malaysian Borneo, with a Focus on the Second Internal Transcribed Spacer (ITS2) of Ribosomal RNA gene
title_full_unstemmed Morphology and Molecular Characterisation of Karenia mikimotoi (Dinophyceae) from Sabah Malaysian Borneo, with a Focus on the Second Internal Transcribed Spacer (ITS2) of Ribosomal RNA gene
title_sort morphology and molecular characterisation of karenia mikimotoi (dinophyceae) from sabah malaysian borneo, with a focus on the second internal transcribed spacer (its2) of ribosomal rna gene
publisher UNIMAS Publisher
publishDate 2024
url http://ir.unimas.my/id/eprint/47109/1/7.%2B%2881-97%29%2BBJRST-590%20%281%29.pdf
http://ir.unimas.my/id/eprint/47109/
https://publisher.unimas.my/ojs/index.php/BJRST/about
https://doi.org/10.33736/bjrst.7417.2024
_version_ 1819914988692701184
score 13.223943

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