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Green-synthesised silver and zinc oxide nanoparticles from stingless bee honey : Morphological characterisation, antimicrobial action, and cytotoxic assessment

This study investigated the green synthesis of silver nanoparticles (Ag-NPs) and zinc oxide nanoparticles (ZnO-NPs) using an aqueous extract of stingless bee honey (SBH) as a reducing and stabilising agent. The rich compositions of SBH containing flavonoids, phenolics, organic acids, sugars, and enz...

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Main Authors: Norfarina, Bahari, Norhashila, Hashim, Khalina, Abdan, Abdah, Md Akim, Bernard, Maringgal, Laith, Al-Shdifat
Format: Article
Language:English
Published: Elsevier Ltd. 2025
Subjects:
Q Science (General)
S Agriculture (General)
T Technology (General)
Online Access:http://ir.unimas.my/id/eprint/46989/3/Green-synthesised%20silver%20and%20zinc%20-%20Copy.pdf
http://ir.unimas.my/id/eprint/46989/
https://www.sciencedirect.com/science/article/pii/S0045653524028698?via%3Dihub
https://doi.org/10.1016/j.chemosphere.2024.143961
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spelling my.unimas.ir-469892024-12-20T08:08:47Z http://ir.unimas.my/id/eprint/46989/ Green-synthesised silver and zinc oxide nanoparticles from stingless bee honey : Morphological characterisation, antimicrobial action, and cytotoxic assessment Norfarina, Bahari Norhashila, Hashim Khalina, Abdan Abdah, Md Akim Bernard, Maringgal Laith, Al-Shdifat Q Science (General) S Agriculture (General) T Technology (General) This study investigated the green synthesis of silver nanoparticles (Ag-NPs) and zinc oxide nanoparticles (ZnO-NPs) using an aqueous extract of stingless bee honey (SBH) as a reducing and stabilising agent. The rich compositions of SBH containing flavonoids, phenolics, organic acids, sugars, and enzymes makes the SBH extract an ideal biocompatible precursor for the NPs synthesis. Physicochemical characterisation of the synthesised NPs was performed using UV–Vis spectroscopy, FESEM, TEM, XRD, and FTIR spectroscopy. The results revealed that the Ag-NPs and ZnO-NPs exhibited polydispersity, with size ranges between 25-50 nm and 15–30 nm, respectively. A majority of the NPs possessed a spherical morphology. Furthermore, the study evaluated the antimicrobial activity of the SBH-based NPs against gram-positive (Staphylococcus aureus, ATCC 43300) and gram-negative (Escherichia coli, ATCC 25922) bacteria. The findings demonstrated significantly higher antimicrobial efficacy of the Ag-NPs with a zone of inhibition (ZOI) of 16.91 mm against S. aureus, and 17.43 mm against E. coli compared to the ZnO-NPs which having a ZOI of 13.05 mm and 14.01 mm, respectively. Notably, cytotoxicity assays revealed no adverse effects of the synthesised NPs on normal mouse fibroblast (3T3) and human lung fibroblast (MRC5) cells up to 100 μg/ml of concentration. These findings suggest the potential of SBH-based Ag-NPs and ZnO-NPs as safe and effective antibacterial agents for various applications, including pharmaceuticals, cosmetics, ointments, and lotions. Elsevier Ltd. 2025-02 Article PeerReviewed text en http://ir.unimas.my/id/eprint/46989/3/Green-synthesised%20silver%20and%20zinc%20-%20Copy.pdf Norfarina, Bahari and Norhashila, Hashim and Khalina, Abdan and Abdah, Md Akim and Bernard, Maringgal and Laith, Al-Shdifat (2025) Green-synthesised silver and zinc oxide nanoparticles from stingless bee honey : Morphological characterisation, antimicrobial action, and cytotoxic assessment. Chemosphere, 370 (143961). pp. 1-12. ISSN 0045-6535 https://www.sciencedirect.com/science/article/pii/S0045653524028698?via%3Dihub https://doi.org/10.1016/j.chemosphere.2024.143961
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
topic Q Science (General)
S Agriculture (General)
T Technology (General)
spellingShingle Q Science (General)
S Agriculture (General)
T Technology (General)
Norfarina, Bahari
Norhashila, Hashim
Khalina, Abdan
Abdah, Md Akim
Bernard, Maringgal
Laith, Al-Shdifat
Green-synthesised silver and zinc oxide nanoparticles from stingless bee honey : Morphological characterisation, antimicrobial action, and cytotoxic assessment
description This study investigated the green synthesis of silver nanoparticles (Ag-NPs) and zinc oxide nanoparticles (ZnO-NPs) using an aqueous extract of stingless bee honey (SBH) as a reducing and stabilising agent. The rich compositions of SBH containing flavonoids, phenolics, organic acids, sugars, and enzymes makes the SBH extract an ideal biocompatible precursor for the NPs synthesis. Physicochemical characterisation of the synthesised NPs was performed using UV–Vis spectroscopy, FESEM, TEM, XRD, and FTIR spectroscopy. The results revealed that the Ag-NPs and ZnO-NPs exhibited polydispersity, with size ranges between 25-50 nm and 15–30 nm, respectively. A majority of the NPs possessed a spherical morphology. Furthermore, the study evaluated the antimicrobial activity of the SBH-based NPs against gram-positive (Staphylococcus aureus, ATCC 43300) and gram-negative (Escherichia coli, ATCC 25922) bacteria. The findings demonstrated significantly higher antimicrobial efficacy of the Ag-NPs with a zone of inhibition (ZOI) of 16.91 mm against S. aureus, and 17.43 mm against E. coli compared to the ZnO-NPs which having a ZOI of 13.05 mm and 14.01 mm, respectively. Notably, cytotoxicity assays revealed no adverse effects of the synthesised NPs on normal mouse fibroblast (3T3) and human lung fibroblast (MRC5) cells up to 100 μg/ml of concentration. These findings suggest the potential of SBH-based Ag-NPs and ZnO-NPs as safe and effective antibacterial agents for various applications, including pharmaceuticals, cosmetics, ointments, and lotions.
format Article
author Norfarina, Bahari
Norhashila, Hashim
Khalina, Abdan
Abdah, Md Akim
Bernard, Maringgal
Laith, Al-Shdifat
author_facet Norfarina, Bahari
Norhashila, Hashim
Khalina, Abdan
Abdah, Md Akim
Bernard, Maringgal
Laith, Al-Shdifat
author_sort Norfarina, Bahari
title Green-synthesised silver and zinc oxide nanoparticles from stingless bee honey : Morphological characterisation, antimicrobial action, and cytotoxic assessment
title_short Green-synthesised silver and zinc oxide nanoparticles from stingless bee honey : Morphological characterisation, antimicrobial action, and cytotoxic assessment
title_full Green-synthesised silver and zinc oxide nanoparticles from stingless bee honey : Morphological characterisation, antimicrobial action, and cytotoxic assessment
title_fullStr Green-synthesised silver and zinc oxide nanoparticles from stingless bee honey : Morphological characterisation, antimicrobial action, and cytotoxic assessment
title_full_unstemmed Green-synthesised silver and zinc oxide nanoparticles from stingless bee honey : Morphological characterisation, antimicrobial action, and cytotoxic assessment
title_sort green-synthesised silver and zinc oxide nanoparticles from stingless bee honey : morphological characterisation, antimicrobial action, and cytotoxic assessment
publisher Elsevier Ltd.
publishDate 2025
url http://ir.unimas.my/id/eprint/46989/3/Green-synthesised%20silver%20and%20zinc%20-%20Copy.pdf
http://ir.unimas.my/id/eprint/46989/
https://www.sciencedirect.com/science/article/pii/S0045653524028698?via%3Dihub
https://doi.org/10.1016/j.chemosphere.2024.143961
_version_ 1819914976559628288
score 13.223943

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