Tapping into Tinospora crispa and Tinospora cordifolia bioactive potentials via Antioxidant, Antiglycation and GC-MS Analysis

Tinospora crispa and Tinospora cordifolia are plant species that are commonly used in traditional medicine, such as Ayurvedic medicine, renowned for their therapeutic roles in addressing diverse health issues, including diabetes. These plants are esteemed for their ability to counter oxidative stres...

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Main Authors: Luqman, Jaya, Zunika, Amit, Teknowilie, Singa, Patrick N., Okechukwu, Mohamad Johari, Ibrahim, Aisha, Mohd Din, Gabriele Ruth Anisah, Froemming
Format: Article
Language:English
Published: The Malaysian Society of Applied Biology 2024
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Online Access:http://ir.unimas.my/id/eprint/46965/1/21-33%2B2%2B2816-1.pdf
http://ir.unimas.my/id/eprint/46965/
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spelling my.unimas.ir-469652024-12-30T01:46:10Z http://ir.unimas.my/id/eprint/46965/ Tapping into Tinospora crispa and Tinospora cordifolia bioactive potentials via Antioxidant, Antiglycation and GC-MS Analysis Luqman, Jaya Zunika, Amit Teknowilie, Singa Patrick N., Okechukwu Mohamad Johari, Ibrahim Aisha, Mohd Din Gabriele Ruth Anisah, Froemming RM Therapeutics. Pharmacology Tinospora crispa and Tinospora cordifolia are plant species that are commonly used in traditional medicine, such as Ayurvedic medicine, renowned for their therapeutic roles in addressing diverse health issues, including diabetes. These plants are esteemed for their ability to counter oxidative stress through electron donation which is a prominent feature of antioxidant. However, a sole assessment of their antioxidant effectiveness is insufficient to holistically understand their antioxidative capabilities. The primary objective of this study was to study the antioxidative and antiglycation properties exhibited by T. crispa and T. cordifolia. This evaluation encompassed a range of tests measuring radical scavenging activity (DPPH assay), capacity for reducing ferric ions (FRAP assay), and their antiglycation potential (BSA-MGO assay). To further enhance this investigation, GC-MS analysis was employed to identify compounds with antioxidative properties within T. crispa and T. cordifolia. The stems and leaves of T. crispa and T. cordifolia underwent solvent extraction using 90% methanol and hot distilled water. Notably, the methanolic extract of T. cordifolia displayed the most robust radical scavenging activity, evident from its lowest IC50 value, 0.03 ± 0.00 mg/mL in the DPPH assay. Conversely, the methanolic extract of T. crispa exhibited the least IC50 value, 0.19 ± 0.00 mg/mL in the FRAP assay. Additionally, the methanolic extract of T. cordifolia showcased a minimal IC50 value 0.52 ± 0.18 mg/mL in the BSA-MGO antiglycation assay. It’s worth noting that the methanolic extracts of both T. crispa and T. cordifolia outperformed their hot water counterparts in terms of antioxidative activity, potentially due to the presence of phytochemical compounds such as phenol, 4-vinyl guaiacol, guaiacol, syringol, and vanillin in the methanolic extracts. The study highlights the potent antioxidative properties of T. crispa and T. cordifolia in supporting their traditional medicinal use and leads the way for the development of antioxidant therapies, particularly for managing oxidative stress-related conditions such as diabetes. The Malaysian Society of Applied Biology 2024-12-25 Article PeerReviewed text en http://ir.unimas.my/id/eprint/46965/1/21-33%2B2%2B2816-1.pdf Luqman, Jaya and Zunika, Amit and Teknowilie, Singa and Patrick N., Okechukwu and Mohamad Johari, Ibrahim and Aisha, Mohd Din and Gabriele Ruth Anisah, Froemming (2024) Tapping into Tinospora crispa and Tinospora cordifolia bioactive potentials via Antioxidant, Antiglycation and GC-MS Analysis. Malaysian Applied Biology, 53 (6). pp. 21-33. ISSN 2462-151X https://jms.mabjournal.com/index.php/mab/about https://doi.org/10.55230/mabjournal.xxxxxxx
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
topic RM Therapeutics. Pharmacology
spellingShingle RM Therapeutics. Pharmacology
Luqman, Jaya
Zunika, Amit
Teknowilie, Singa
Patrick N., Okechukwu
Mohamad Johari, Ibrahim
Aisha, Mohd Din
Gabriele Ruth Anisah, Froemming
Tapping into Tinospora crispa and Tinospora cordifolia bioactive potentials via Antioxidant, Antiglycation and GC-MS Analysis
description Tinospora crispa and Tinospora cordifolia are plant species that are commonly used in traditional medicine, such as Ayurvedic medicine, renowned for their therapeutic roles in addressing diverse health issues, including diabetes. These plants are esteemed for their ability to counter oxidative stress through electron donation which is a prominent feature of antioxidant. However, a sole assessment of their antioxidant effectiveness is insufficient to holistically understand their antioxidative capabilities. The primary objective of this study was to study the antioxidative and antiglycation properties exhibited by T. crispa and T. cordifolia. This evaluation encompassed a range of tests measuring radical scavenging activity (DPPH assay), capacity for reducing ferric ions (FRAP assay), and their antiglycation potential (BSA-MGO assay). To further enhance this investigation, GC-MS analysis was employed to identify compounds with antioxidative properties within T. crispa and T. cordifolia. The stems and leaves of T. crispa and T. cordifolia underwent solvent extraction using 90% methanol and hot distilled water. Notably, the methanolic extract of T. cordifolia displayed the most robust radical scavenging activity, evident from its lowest IC50 value, 0.03 ± 0.00 mg/mL in the DPPH assay. Conversely, the methanolic extract of T. crispa exhibited the least IC50 value, 0.19 ± 0.00 mg/mL in the FRAP assay. Additionally, the methanolic extract of T. cordifolia showcased a minimal IC50 value 0.52 ± 0.18 mg/mL in the BSA-MGO antiglycation assay. It’s worth noting that the methanolic extracts of both T. crispa and T. cordifolia outperformed their hot water counterparts in terms of antioxidative activity, potentially due to the presence of phytochemical compounds such as phenol, 4-vinyl guaiacol, guaiacol, syringol, and vanillin in the methanolic extracts. The study highlights the potent antioxidative properties of T. crispa and T. cordifolia in supporting their traditional medicinal use and leads the way for the development of antioxidant therapies, particularly for managing oxidative stress-related conditions such as diabetes.
format Article
author Luqman, Jaya
Zunika, Amit
Teknowilie, Singa
Patrick N., Okechukwu
Mohamad Johari, Ibrahim
Aisha, Mohd Din
Gabriele Ruth Anisah, Froemming
author_facet Luqman, Jaya
Zunika, Amit
Teknowilie, Singa
Patrick N., Okechukwu
Mohamad Johari, Ibrahim
Aisha, Mohd Din
Gabriele Ruth Anisah, Froemming
author_sort Luqman, Jaya
title Tapping into Tinospora crispa and Tinospora cordifolia bioactive potentials via Antioxidant, Antiglycation and GC-MS Analysis
title_short Tapping into Tinospora crispa and Tinospora cordifolia bioactive potentials via Antioxidant, Antiglycation and GC-MS Analysis
title_full Tapping into Tinospora crispa and Tinospora cordifolia bioactive potentials via Antioxidant, Antiglycation and GC-MS Analysis
title_fullStr Tapping into Tinospora crispa and Tinospora cordifolia bioactive potentials via Antioxidant, Antiglycation and GC-MS Analysis
title_full_unstemmed Tapping into Tinospora crispa and Tinospora cordifolia bioactive potentials via Antioxidant, Antiglycation and GC-MS Analysis
title_sort tapping into tinospora crispa and tinospora cordifolia bioactive potentials via antioxidant, antiglycation and gc-ms analysis
publisher The Malaysian Society of Applied Biology
publishDate 2024
url http://ir.unimas.my/id/eprint/46965/1/21-33%2B2%2B2816-1.pdf
http://ir.unimas.my/id/eprint/46965/
https://jms.mabjournal.com/index.php/mab/about
https://doi.org/10.55230/mabjournal.xxxxxxx
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score 13.235796