Cover Image

Selection of DNA aptamers against Chikungunya virus Envelope 2 Protein and their application in sandwich ELASA

Chikungunya fever, caused by Chikungunya virus (CHIKV) exhibits clinical features that mimic that of other arbovirus infections such as dengue. CHIKV Envelope 2 (E2) protein, an antigenic epitope of CHIKV, has been identified as an ideal marker for diagnostics. The current CHIKV antigen detection...

Full description

Saved in:
Bibliographic Details
Main Authors: Anna, Andrew, Magdline Sia, Henry Sum, Ch'ng, Ewe Seng, Tang, Thean Hock, Citartan, Marimuthu
Format: Article
Language:English
Published: Elsevier B.V. 2025
Subjects:
QR Microbiology
QR355 Virology
Online Access:http://ir.unimas.my/id/eprint/46913/1/Selection%20of%20DNA%20aptamers%20against%20Chikungunya%20-%20Copy.pdf
http://ir.unimas.my/id/eprint/46913/
https://www.sciencedirect.com/science/article/abs/pii/S0039914024012219?via%3Dihub
https://doi.org/10.1016/j.talanta.2024.126842
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.unimas.ir-46913
record_format eprints
spelling my.unimas.ir-469132024-12-16T02:16:22Z http://ir.unimas.my/id/eprint/46913/ Selection of DNA aptamers against Chikungunya virus Envelope 2 Protein and their application in sandwich ELASA Anna, Andrew Magdline Sia, Henry Sum Ch'ng, Ewe Seng Tang, Thean Hock Citartan, Marimuthu QR Microbiology QR355 Virology Chikungunya fever, caused by Chikungunya virus (CHIKV) exhibits clinical features that mimic that of other arbovirus infections such as dengue. CHIKV Envelope 2 (E2) protein, an antigenic epitope of CHIKV, has been identified as an ideal marker for diagnostics. The current CHIKV antigen detection tests are largely based on antibodies but are beleaguered by issues such as sensitivity to high temperature, expensive and prone to batch-tobatch variations. Aptamers are suitable alternatives to antibodies as they are cheaper and have no batch-to-batch variations compared to antibodies. In this study, DNA aptamer selection against CHIKV E2 proteins was performed using two different randomized ssDNA libraries. Chik-2 (96-mer) and Chik-3 (76-mer) were isolated from these two libraries and were identified as the potential aptamers against CHIKV E2 protein. The binding affinity of Chik-2 and Chik-3 against CHIKV E2 protein was estimated at 177.5 ± 32.69 nM and 30.01 ± 3.60 nM, respectively. A sandwich ELASA was developed, and this assay showed a detection limit of 2.17 x 103 PFU/mL. The sensitivity and specificity of the assay were 80 % and 100 %, respectively. The assay showed no crossreactivity with dengue-positive samples, demonstrating the enormous diagnostic potential of these aptamers for the detection of CHIKV. Elsevier B.V. 2025 Article PeerReviewed text en http://ir.unimas.my/id/eprint/46913/1/Selection%20of%20DNA%20aptamers%20against%20Chikungunya%20-%20Copy.pdf Anna, Andrew and Magdline Sia, Henry Sum and Ch'ng, Ewe Seng and Tang, Thean Hock and Citartan, Marimuthu (2025) Selection of DNA aptamers against Chikungunya virus Envelope 2 Protein and their application in sandwich ELASA. Talanta, 281. pp. 1-10. ISSN 0039-9140 https://www.sciencedirect.com/science/article/abs/pii/S0039914024012219?via%3Dihub https://doi.org/10.1016/j.talanta.2024.126842
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
topic QR Microbiology
QR355 Virology
spellingShingle QR Microbiology
QR355 Virology
Anna, Andrew
Magdline Sia, Henry Sum
Ch'ng, Ewe Seng
Tang, Thean Hock
Citartan, Marimuthu
Selection of DNA aptamers against Chikungunya virus Envelope 2 Protein and their application in sandwich ELASA
description Chikungunya fever, caused by Chikungunya virus (CHIKV) exhibits clinical features that mimic that of other arbovirus infections such as dengue. CHIKV Envelope 2 (E2) protein, an antigenic epitope of CHIKV, has been identified as an ideal marker for diagnostics. The current CHIKV antigen detection tests are largely based on antibodies but are beleaguered by issues such as sensitivity to high temperature, expensive and prone to batch-tobatch variations. Aptamers are suitable alternatives to antibodies as they are cheaper and have no batch-to-batch variations compared to antibodies. In this study, DNA aptamer selection against CHIKV E2 proteins was performed using two different randomized ssDNA libraries. Chik-2 (96-mer) and Chik-3 (76-mer) were isolated from these two libraries and were identified as the potential aptamers against CHIKV E2 protein. The binding affinity of Chik-2 and Chik-3 against CHIKV E2 protein was estimated at 177.5 ± 32.69 nM and 30.01 ± 3.60 nM, respectively. A sandwich ELASA was developed, and this assay showed a detection limit of 2.17 x 103 PFU/mL. The sensitivity and specificity of the assay were 80 % and 100 %, respectively. The assay showed no crossreactivity with dengue-positive samples, demonstrating the enormous diagnostic potential of these aptamers for the detection of CHIKV.
format Article
author Anna, Andrew
Magdline Sia, Henry Sum
Ch'ng, Ewe Seng
Tang, Thean Hock
Citartan, Marimuthu
author_facet Anna, Andrew
Magdline Sia, Henry Sum
Ch'ng, Ewe Seng
Tang, Thean Hock
Citartan, Marimuthu
author_sort Anna, Andrew
title Selection of DNA aptamers against Chikungunya virus Envelope 2 Protein and their application in sandwich ELASA
title_short Selection of DNA aptamers against Chikungunya virus Envelope 2 Protein and their application in sandwich ELASA
title_full Selection of DNA aptamers against Chikungunya virus Envelope 2 Protein and their application in sandwich ELASA
title_fullStr Selection of DNA aptamers against Chikungunya virus Envelope 2 Protein and their application in sandwich ELASA
title_full_unstemmed Selection of DNA aptamers against Chikungunya virus Envelope 2 Protein and their application in sandwich ELASA
title_sort selection of dna aptamers against chikungunya virus envelope 2 protein and their application in sandwich elasa
publisher Elsevier B.V.
publishDate 2025
url http://ir.unimas.my/id/eprint/46913/1/Selection%20of%20DNA%20aptamers%20against%20Chikungunya%20-%20Copy.pdf
http://ir.unimas.my/id/eprint/46913/
https://www.sciencedirect.com/science/article/abs/pii/S0039914024012219?via%3Dihub
https://doi.org/10.1016/j.talanta.2024.126842
_version_ 1818839393532641280
score 13.222552

Similar Items