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Preliminary Work on Morphological Assessment & Total Genomic DNA Isolation of Sarawak Razor Clam, (Solen spp. ) in Kuching, Bay

The Sarawak razor clam or locally known as `Ambal' has been divided into three different species: Solen regularis Dunker 1962 (Ambal Biasa), Solen vagina Linne 1758 (Ambal Jernang) and Solen sarawakensis von Cosel. 2002 (Ambal Riong). These razor clams are well characterized by the differen...

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Bibliographic Details
Main Author: Hung, Tze Mau
Format: Final Year Project Report
Language:English
Published: Universiti Malaysia Sarawak, (UNIMAS) 2006
Subjects:
SH Aquaculture. Fisheries. Angling
Online Access:http://ir.unimas.my/id/eprint/46753/1/Hung%20Tze%20Mau%20ft.pdf
http://ir.unimas.my/id/eprint/46753/
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spelling my.unimas.ir-467532024-11-29T08:06:39Z http://ir.unimas.my/id/eprint/46753/ Preliminary Work on Morphological Assessment & Total Genomic DNA Isolation of Sarawak Razor Clam, (Solen spp. ) in Kuching, Bay Hung, Tze Mau SH Aquaculture. Fisheries. Angling The Sarawak razor clam or locally known as `Ambal' has been divided into three different species: Solen regularis Dunker 1962 (Ambal Biasa), Solen vagina Linne 1758 (Ambal Jernang) and Solen sarawakensis von Cosel. 2002 (Ambal Riong). These razor clams are well characterized by the different outlines, the arrangement of the adductor scars and pallial muscle scars. Thus, preliminary assessment on morphological characters such as weight, shell length and width were measured using the method proposed by (Pang, 1994). Moreover the arrangement of the adductor and pallial muscle scar were assessed and recorded. In comparison to other types of infaunal bivalves families such as Cardiidae and Psammobidae, the Solenacea are not species rich, but they are commercially important because there is a high demand in the seafood industries for domestic consumption. Currently, Solen spp taxonomy is solely based on morphological description. This study is an attempt to validate it using molecular data. There are problems of misidentification that therefore limit razor clams studies. Genomic DNA has been successfully extracted from the three Solen species found in Kuching Bay area using a modified version C-TAB protocol. Amplification of COI and partial cyt b mtDNA gene was unsuccessful and need further research. Modifications were done on homogenisation method and centrifugation time. Universiti Malaysia Sarawak, (UNIMAS) 2006 Final Year Project Report NonPeerReviewed text en http://ir.unimas.my/id/eprint/46753/1/Hung%20Tze%20Mau%20ft.pdf Hung, Tze Mau (2006) Preliminary Work on Morphological Assessment & Total Genomic DNA Isolation of Sarawak Razor Clam, (Solen spp. ) in Kuching, Bay. [Final Year Project Report] (Unpublished)
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
topic SH Aquaculture. Fisheries. Angling
spellingShingle SH Aquaculture. Fisheries. Angling
Hung, Tze Mau
Preliminary Work on Morphological Assessment & Total Genomic DNA Isolation of Sarawak Razor Clam, (Solen spp. ) in Kuching, Bay
description The Sarawak razor clam or locally known as `Ambal' has been divided into three different species: Solen regularis Dunker 1962 (Ambal Biasa), Solen vagina Linne 1758 (Ambal Jernang) and Solen sarawakensis von Cosel. 2002 (Ambal Riong). These razor clams are well characterized by the different outlines, the arrangement of the adductor scars and pallial muscle scars. Thus, preliminary assessment on morphological characters such as weight, shell length and width were measured using the method proposed by (Pang, 1994). Moreover the arrangement of the adductor and pallial muscle scar were assessed and recorded. In comparison to other types of infaunal bivalves families such as Cardiidae and Psammobidae, the Solenacea are not species rich, but they are commercially important because there is a high demand in the seafood industries for domestic consumption. Currently, Solen spp taxonomy is solely based on morphological description. This study is an attempt to validate it using molecular data. There are problems of misidentification that therefore limit razor clams studies. Genomic DNA has been successfully extracted from the three Solen species found in Kuching Bay area using a modified version C-TAB protocol. Amplification of COI and partial cyt b mtDNA gene was unsuccessful and need further research. Modifications were done on homogenisation method and centrifugation time.
format Final Year Project Report
author Hung, Tze Mau
author_facet Hung, Tze Mau
author_sort Hung, Tze Mau
title Preliminary Work on Morphological Assessment & Total Genomic DNA Isolation of Sarawak Razor Clam, (Solen spp. ) in Kuching, Bay
title_short Preliminary Work on Morphological Assessment & Total Genomic DNA Isolation of Sarawak Razor Clam, (Solen spp. ) in Kuching, Bay
title_full Preliminary Work on Morphological Assessment & Total Genomic DNA Isolation of Sarawak Razor Clam, (Solen spp. ) in Kuching, Bay
title_fullStr Preliminary Work on Morphological Assessment & Total Genomic DNA Isolation of Sarawak Razor Clam, (Solen spp. ) in Kuching, Bay
title_full_unstemmed Preliminary Work on Morphological Assessment & Total Genomic DNA Isolation of Sarawak Razor Clam, (Solen spp. ) in Kuching, Bay
title_sort preliminary work on morphological assessment & total genomic dna isolation of sarawak razor clam, (solen spp. ) in kuching, bay
publisher Universiti Malaysia Sarawak, (UNIMAS)
publishDate 2006
url http://ir.unimas.my/id/eprint/46753/1/Hung%20Tze%20Mau%20ft.pdf
http://ir.unimas.my/id/eprint/46753/
_version_ 1817848776428093440
score 13.244413

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