Staff View: Cell-mediated and humoral immune responses of cyprinids induced by a live attenuated vaccine against cyprinid herpesvirus 2 infection in comparison to the virus non-permissive high temperature water treatment

Cell-mediated and humoral immune responses of cyprinids induced by a live attenuated vaccine against cyprinid herpesvirus 2 infection in comparison to the virus non-permissive high temperature water treatment

The live attenuated vaccine (P7-P8 strain) against cyprinid herpesvirus 2 (CyHV-2) infection of goldfish shows high protective efficacy. However, the underlying immune mechanism induced by P7-P8 vaccination remains unknown. It is known that the fish survived in the primary infection with CyHV-2 by t...

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Main Authors:	Hiroaki, Saito, Lau, Lik Ming, Shungo, Minami, Manami, Yuguchi, Megumi, Matsumoto, Teruyuki, Nakanishi, Hidehiro, Kondo, Goshi, Kato, Motohiko, Sano
Format:	Article
Language:	English
Published:	Elsevier Ltd. 2024
Subjects:	SH Aquaculture. Fisheries. Angling
Online Access:	http://ir.unimas.my/id/eprint/46533/4/Cell-mediated%20and%20humoral%20immune%20-%20Copy.pdf http://ir.unimas.my/id/eprint/46533/ https://www.sciencedirect.com/science/article/abs/pii/S1050464824006363 https://doi.org/10.1016/j.fsi.2024.109991
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spelling	my.unimas.ir-465332024-11-04T01:47:52Z http://ir.unimas.my/id/eprint/46533/ Cell-mediated and humoral immune responses of cyprinids induced by a live attenuated vaccine against cyprinid herpesvirus 2 infection in comparison to the virus non-permissive high temperature water treatment Hiroaki, Saito Lau, Lik Ming Shungo, Minami Manami, Yuguchi Megumi, Matsumoto Teruyuki, Nakanishi Hidehiro, Kondo Goshi, Kato Motohiko, Sano SH Aquaculture. Fisheries. Angling The live attenuated vaccine (P7-P8 strain) against cyprinid herpesvirus 2 (CyHV-2) infection of goldfish shows high protective efficacy. However, the underlying immune mechanism induced by P7-P8 vaccination remains unknown. It is known that the fish survived in the primary infection with CyHV-2 by the virus non-permissive high temperature (HT) water treatment elicit immunity against secondary virus challenge. In this study, the immunity induced by the P7-P8 vaccine was compared with that by HT treatment. To further explore the immunological responses of cyprinids, in addition to goldfish Carassius auratus, susceptible isogenic ginbuna C. auratus langsdorfii was included in this study. In the primary immune response, cyprinids were vaccinated with P7-P8, or treated with HT. In the secondary immune response, cyprinids were challenged with the virulent CyHV-2. The percentage dynamics of CD4-1 and CD8α positive lymphocytes were determined during the primary and secondary immune responses of the two cyprinids. Blood plasma was sampled to assess the anti-CyHV-2 IgM antibody titers. The vaccination with P7-P8 and HT-treatment induced high protection immunity in the cyprinids with relative percentage survival of over 88 % against virulent virus challenge. Our finding shows that the CD8α positive lymphocytes rather than CD4-1 positive lymphocytes play an important role in the secondary immune responses of cyprinids vaccinated with P7-P8. The CD4-1 positive lymphocytes rather than CD8α positive lymphocytes play an important role in the secondary immune responses of cyprinids treated with HT. The antibody titer of vaccinated cyprinids did not increase greatly even after virulent virus challenge. The results suggest the vaccine activates the CD8α cells and the secondary cell-mediated immunity. The differences in the induced immunity mechanisms in fish by the two measures might be based on the virus either being an avirulent virus form that cannot evade host responses or a virulent virus form that cannot propagate at non-permissive temperature. Elsevier Ltd. 2024 Article PeerReviewed text en http://ir.unimas.my/id/eprint/46533/4/Cell-mediated%20and%20humoral%20immune%20-%20Copy.pdf Hiroaki, Saito and Lau, Lik Ming and Shungo, Minami and Manami, Yuguchi and Megumi, Matsumoto and Teruyuki, Nakanishi and Hidehiro, Kondo and Goshi, Kato and Motohiko, Sano (2024) Cell-mediated and humoral immune responses of cyprinids induced by a live attenuated vaccine against cyprinid herpesvirus 2 infection in comparison to the virus non-permissive high temperature water treatment. Fish & Shellfish Immunology, 154. p. 109991. ISSN 1050-4648 https://www.sciencedirect.com/science/article/abs/pii/S1050464824006363 https://doi.org/10.1016/j.fsi.2024.109991
institution	Universiti Malaysia Sarawak
building	Centre for Academic Information Services (CAIS)
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Malaysia Sarawak
content_source	UNIMAS Institutional Repository
url_provider	http://ir.unimas.my/
language	English
topic	SH Aquaculture. Fisheries. Angling
spellingShingle	SH Aquaculture. Fisheries. Angling Hiroaki, Saito Lau, Lik Ming Shungo, Minami Manami, Yuguchi Megumi, Matsumoto Teruyuki, Nakanishi Hidehiro, Kondo Goshi, Kato Motohiko, Sano Cell-mediated and humoral immune responses of cyprinids induced by a live attenuated vaccine against cyprinid herpesvirus 2 infection in comparison to the virus non-permissive high temperature water treatment
description	The live attenuated vaccine (P7-P8 strain) against cyprinid herpesvirus 2 (CyHV-2) infection of goldfish shows high protective efficacy. However, the underlying immune mechanism induced by P7-P8 vaccination remains unknown. It is known that the fish survived in the primary infection with CyHV-2 by the virus non-permissive high temperature (HT) water treatment elicit immunity against secondary virus challenge. In this study, the immunity induced by the P7-P8 vaccine was compared with that by HT treatment. To further explore the immunological responses of cyprinids, in addition to goldfish Carassius auratus, susceptible isogenic ginbuna C. auratus langsdorfii was included in this study. In the primary immune response, cyprinids were vaccinated with P7-P8, or treated with HT. In the secondary immune response, cyprinids were challenged with the virulent CyHV-2. The percentage dynamics of CD4-1 and CD8α positive lymphocytes were determined during the primary and secondary immune responses of the two cyprinids. Blood plasma was sampled to assess the anti-CyHV-2 IgM antibody titers. The vaccination with P7-P8 and HT-treatment induced high protection immunity in the cyprinids with relative percentage survival of over 88 % against virulent virus challenge. Our finding shows that the CD8α positive lymphocytes rather than CD4-1 positive lymphocytes play an important role in the secondary immune responses of cyprinids vaccinated with P7-P8. The CD4-1 positive lymphocytes rather than CD8α positive lymphocytes play an important role in the secondary immune responses of cyprinids treated with HT. The antibody titer of vaccinated cyprinids did not increase greatly even after virulent virus challenge. The results suggest the vaccine activates the CD8α cells and the secondary cell-mediated immunity. The differences in the induced immunity mechanisms in fish by the two measures might be based on the virus either being an avirulent virus form that cannot evade host responses or a virulent virus form that cannot propagate at non-permissive temperature.
format	Article
author	Hiroaki, Saito Lau, Lik Ming Shungo, Minami Manami, Yuguchi Megumi, Matsumoto Teruyuki, Nakanishi Hidehiro, Kondo Goshi, Kato Motohiko, Sano
author_facet	Hiroaki, Saito Lau, Lik Ming Shungo, Minami Manami, Yuguchi Megumi, Matsumoto Teruyuki, Nakanishi Hidehiro, Kondo Goshi, Kato Motohiko, Sano
author_sort	Hiroaki, Saito
title	Cell-mediated and humoral immune responses of cyprinids induced by a live attenuated vaccine against cyprinid herpesvirus 2 infection in comparison to the virus non-permissive high temperature water treatment
title_short	Cell-mediated and humoral immune responses of cyprinids induced by a live attenuated vaccine against cyprinid herpesvirus 2 infection in comparison to the virus non-permissive high temperature water treatment
title_full	Cell-mediated and humoral immune responses of cyprinids induced by a live attenuated vaccine against cyprinid herpesvirus 2 infection in comparison to the virus non-permissive high temperature water treatment
title_fullStr	Cell-mediated and humoral immune responses of cyprinids induced by a live attenuated vaccine against cyprinid herpesvirus 2 infection in comparison to the virus non-permissive high temperature water treatment
title_full_unstemmed	Cell-mediated and humoral immune responses of cyprinids induced by a live attenuated vaccine against cyprinid herpesvirus 2 infection in comparison to the virus non-permissive high temperature water treatment
title_sort	cell-mediated and humoral immune responses of cyprinids induced by a live attenuated vaccine against cyprinid herpesvirus 2 infection in comparison to the virus non-permissive high temperature water treatment
publisher	Elsevier Ltd.
publishDate	2024
url	http://ir.unimas.my/id/eprint/46533/4/Cell-mediated%20and%20humoral%20immune%20-%20Copy.pdf http://ir.unimas.my/id/eprint/46533/ https://www.sciencedirect.com/science/article/abs/pii/S1050464824006363 https://doi.org/10.1016/j.fsi.2024.109991
_version_	1814942189334036480
score	13.244413

Cell-mediated and humoral immune responses of cyprinids induced by a live attenuated vaccine against cyprinid herpesvirus 2 infection in comparison to the virus non-permissive high temperature water treatment

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