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Simultaneous detection of Vibrio spp. in post-larvae stocking to harvest process of shrimps production in commercialised farms in Sarawak

Aims: The shrimp farming industry faces persistent challenges stemming from infectious diseases and environmental issues. The aim of this study was to monitor and assess the risk associated with the distribution of Vibrio spp. throughout the entire shrimp production cycle, spanning from the first da...

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Main Authors: Elexson, Nillian, Dalene, Lesenand, Nur Diyana, Zakaria
Format: Article
Language:English
Published: Malaysian Society for Microbiology 2024
Subjects:
QR Microbiology
Online Access:http://ir.unimas.my/id/eprint/46234/1/Simultaneous%20detection%20of%20Vibrio.pdf
http://ir.unimas.my/id/eprint/46234/
https://mjm.usm.my/uploads/issues/1963/Formatted%20MJM2-23-0514%E2%80%93ready%20colour.pdf
http://dx.doi.org/10.21161/mjm.230514
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spelling my.unimas.ir-462342024-10-07T01:00:52Z http://ir.unimas.my/id/eprint/46234/ Simultaneous detection of Vibrio spp. in post-larvae stocking to harvest process of shrimps production in commercialised farms in Sarawak Elexson, Nillian Dalene, Lesenand Nur Diyana, Zakaria QR Microbiology Aims: The shrimp farming industry faces persistent challenges stemming from infectious diseases and environmental issues. The aim of this study was to monitor and assess the risk associated with the distribution of Vibrio spp. throughout the entire shrimp production cycle, spanning from the first day of culture (day 1) to the 98th day of culture (day 98). Methodology and results: The sampling was done by collecting water samples (n=48) and sediment samples (n=48) from three different locations: Telaga Air, Santubong and Tanjung Manis. Utilizing multiplex PCR with three specific primer sets, three Vibrio species were identified. Prevalence rates for V. parahaemolyticus were found to be 81.25% in water and 52.08% in sediment samples, while V. alginolyticus was detected in 16.67% of water samples and 27.08% of sediment samples. Vibrio cholerae was present in 10.42% of water samples and 25.0% of sediment samples. Furthermore, an antimicrobial susceptibility test (AST) was conducted on the Vibrio spp. isolates obtained from the three shrimp farms using 20 common antibiotics. Penicillin displayed the highest resistance rate at 40%, followed by streptomycin at 30%. The study also calculated the MAR index, which exhibited a range from 0.35 to 0.65. Conclusion, significance and impact of study: These research findings provide valuable insights into the distribution of Vibrio spp. in Sarawak aquaculture shrimp farms, which can help in preventing and controlling the possible occurrence of vibriosis. Malaysian Society for Microbiology 2024 Article PeerReviewed text en http://ir.unimas.my/id/eprint/46234/1/Simultaneous%20detection%20of%20Vibrio.pdf Elexson, Nillian and Dalene, Lesenand and Nur Diyana, Zakaria (2024) Simultaneous detection of Vibrio spp. in post-larvae stocking to harvest process of shrimps production in commercialised farms in Sarawak. Malaysian Journal of Microbiology, 20 (5). pp. 636-651. ISSN 2231-7538 https://mjm.usm.my/uploads/issues/1963/Formatted%20MJM2-23-0514%E2%80%93ready%20colour.pdf http://dx.doi.org/10.21161/mjm.230514
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
topic QR Microbiology
spellingShingle QR Microbiology
Elexson, Nillian
Dalene, Lesenand
Nur Diyana, Zakaria
Simultaneous detection of Vibrio spp. in post-larvae stocking to harvest process of shrimps production in commercialised farms in Sarawak
description Aims: The shrimp farming industry faces persistent challenges stemming from infectious diseases and environmental issues. The aim of this study was to monitor and assess the risk associated with the distribution of Vibrio spp. throughout the entire shrimp production cycle, spanning from the first day of culture (day 1) to the 98th day of culture (day 98). Methodology and results: The sampling was done by collecting water samples (n=48) and sediment samples (n=48) from three different locations: Telaga Air, Santubong and Tanjung Manis. Utilizing multiplex PCR with three specific primer sets, three Vibrio species were identified. Prevalence rates for V. parahaemolyticus were found to be 81.25% in water and 52.08% in sediment samples, while V. alginolyticus was detected in 16.67% of water samples and 27.08% of sediment samples. Vibrio cholerae was present in 10.42% of water samples and 25.0% of sediment samples. Furthermore, an antimicrobial susceptibility test (AST) was conducted on the Vibrio spp. isolates obtained from the three shrimp farms using 20 common antibiotics. Penicillin displayed the highest resistance rate at 40%, followed by streptomycin at 30%. The study also calculated the MAR index, which exhibited a range from 0.35 to 0.65. Conclusion, significance and impact of study: These research findings provide valuable insights into the distribution of Vibrio spp. in Sarawak aquaculture shrimp farms, which can help in preventing and controlling the possible occurrence of vibriosis.
format Article
author Elexson, Nillian
Dalene, Lesenand
Nur Diyana, Zakaria
author_facet Elexson, Nillian
Dalene, Lesenand
Nur Diyana, Zakaria
author_sort Elexson, Nillian
title Simultaneous detection of Vibrio spp. in post-larvae stocking to harvest process of shrimps production in commercialised farms in Sarawak
title_short Simultaneous detection of Vibrio spp. in post-larvae stocking to harvest process of shrimps production in commercialised farms in Sarawak
title_full Simultaneous detection of Vibrio spp. in post-larvae stocking to harvest process of shrimps production in commercialised farms in Sarawak
title_fullStr Simultaneous detection of Vibrio spp. in post-larvae stocking to harvest process of shrimps production in commercialised farms in Sarawak
title_full_unstemmed Simultaneous detection of Vibrio spp. in post-larvae stocking to harvest process of shrimps production in commercialised farms in Sarawak
title_sort simultaneous detection of vibrio spp. in post-larvae stocking to harvest process of shrimps production in commercialised farms in sarawak
publisher Malaysian Society for Microbiology
publishDate 2024
url http://ir.unimas.my/id/eprint/46234/1/Simultaneous%20detection%20of%20Vibrio.pdf
http://ir.unimas.my/id/eprint/46234/
https://mjm.usm.my/uploads/issues/1963/Formatted%20MJM2-23-0514%E2%80%93ready%20colour.pdf
http://dx.doi.org/10.21161/mjm.230514
_version_ 1814942137069862912
score 13.223943

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