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Multiplex PCR Assay for the Identification of Four Species of the Anopheles Leucosphyrus Sub-Group in Malaysia. Insects 2022, 13, 195

The Leucosphyrus Group of mosquitoes are the major simian malaria vectors in Malaysia. Accurate species identification is required to help in curbing the spread of simian malaria. The aim of the study is to provide an accurate molecular method for identifying the four important Anopheles vector s...

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Main Authors: Sandthya, Pramasivan, Jonathan Wee Kent, Liew, Nantha Kuma, Jeyaprakasam, Van, Lun Low, Romano, Ngui, Indra, Vythilingam
Format: Article
Language:English
Published: MDPI 2022
Subjects:
QR Microbiology
R Medicine (General)
Online Access:http://ir.unimas.my/id/eprint/42047/1/Correction.pdf
http://ir.unimas.my/id/eprint/42047/
https://www.mdpi.com/2075-4450/13/5/466
https://doi.org/10.3390/insects13050466
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spelling my.unimas.ir-420472025-01-21T02:49:27Z http://ir.unimas.my/id/eprint/42047/ Multiplex PCR Assay for the Identification of Four Species of the Anopheles Leucosphyrus Sub-Group in Malaysia. Insects 2022, 13, 195 Sandthya, Pramasivan Jonathan Wee Kent, Liew Nantha Kuma, Jeyaprakasam Van, Lun Low Romano, Ngui Indra, Vythilingam QR Microbiology R Medicine (General) The Leucosphyrus Group of mosquitoes are the major simian malaria vectors in Malaysia. Accurate species identification is required to help in curbing the spread of simian malaria. The aim of the study is to provide an accurate molecular method for identifying the four important Anopheles vector species found in Malaysia. Mosquito specimens were collected from various localities in Ma- laysia, where simian malaria cases were reported. DNA from 122 mosquito specimens was tested to develop a multiplex polymerase chain reaction (PCR) assay. The specificity of this assay was tested against other mosquito species. Molecular identification of the species was further confirmed by analysing the internal transcribed spacer 2 (ITS2) DNA region of the specimens. Anopheles bala- bacensis and An. latens showed two distinct clades in the phylogenetic tree. The multiplex PCR assay was developed based on the ITS2 region for the identification of Anopheles introlatus (298–299 bp), Anopheles latens (197–198 bp), Anopheles cracens (421–426 bp), and Anopheles balabacensis (224–228 bp). This method will be useful to accurately identify the major Anopheles Leucosphyrus Group species in Malaysia, which are difficult to identify morphologically, to determine the correct vector as well as its geographical distribution MDPI 2022 Article PeerReviewed text en http://ir.unimas.my/id/eprint/42047/1/Correction.pdf Sandthya, Pramasivan and Jonathan Wee Kent, Liew and Nantha Kuma, Jeyaprakasam and Van, Lun Low and Romano, Ngui and Indra, Vythilingam (2022) Multiplex PCR Assay for the Identification of Four Species of the Anopheles Leucosphyrus Sub-Group in Malaysia. Insects 2022, 13, 195. Insects, 13 (5). pp. 1-12. https://www.mdpi.com/2075-4450/13/5/466 https://doi.org/10.3390/insects13050466
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
topic QR Microbiology
R Medicine (General)
spellingShingle QR Microbiology
R Medicine (General)
Sandthya, Pramasivan
Jonathan Wee Kent, Liew
Nantha Kuma, Jeyaprakasam
Van, Lun Low
Romano, Ngui
Indra, Vythilingam
Multiplex PCR Assay for the Identification of Four Species of the Anopheles Leucosphyrus Sub-Group in Malaysia. Insects 2022, 13, 195
description The Leucosphyrus Group of mosquitoes are the major simian malaria vectors in Malaysia. Accurate species identification is required to help in curbing the spread of simian malaria. The aim of the study is to provide an accurate molecular method for identifying the four important Anopheles vector species found in Malaysia. Mosquito specimens were collected from various localities in Ma- laysia, where simian malaria cases were reported. DNA from 122 mosquito specimens was tested to develop a multiplex polymerase chain reaction (PCR) assay. The specificity of this assay was tested against other mosquito species. Molecular identification of the species was further confirmed by analysing the internal transcribed spacer 2 (ITS2) DNA region of the specimens. Anopheles bala- bacensis and An. latens showed two distinct clades in the phylogenetic tree. The multiplex PCR assay was developed based on the ITS2 region for the identification of Anopheles introlatus (298–299 bp), Anopheles latens (197–198 bp), Anopheles cracens (421–426 bp), and Anopheles balabacensis (224–228 bp). This method will be useful to accurately identify the major Anopheles Leucosphyrus Group species in Malaysia, which are difficult to identify morphologically, to determine the correct vector as well as its geographical distribution
format Article
author Sandthya, Pramasivan
Jonathan Wee Kent, Liew
Nantha Kuma, Jeyaprakasam
Van, Lun Low
Romano, Ngui
Indra, Vythilingam
author_facet Sandthya, Pramasivan
Jonathan Wee Kent, Liew
Nantha Kuma, Jeyaprakasam
Van, Lun Low
Romano, Ngui
Indra, Vythilingam
author_sort Sandthya, Pramasivan
title Multiplex PCR Assay for the Identification of Four Species of the Anopheles Leucosphyrus Sub-Group in Malaysia. Insects 2022, 13, 195
title_short Multiplex PCR Assay for the Identification of Four Species of the Anopheles Leucosphyrus Sub-Group in Malaysia. Insects 2022, 13, 195
title_full Multiplex PCR Assay for the Identification of Four Species of the Anopheles Leucosphyrus Sub-Group in Malaysia. Insects 2022, 13, 195
title_fullStr Multiplex PCR Assay for the Identification of Four Species of the Anopheles Leucosphyrus Sub-Group in Malaysia. Insects 2022, 13, 195
title_full_unstemmed Multiplex PCR Assay for the Identification of Four Species of the Anopheles Leucosphyrus Sub-Group in Malaysia. Insects 2022, 13, 195
title_sort multiplex pcr assay for the identification of four species of the anopheles leucosphyrus sub-group in malaysia. insects 2022, 13, 195
publisher MDPI
publishDate 2022
url http://ir.unimas.my/id/eprint/42047/1/Correction.pdf
http://ir.unimas.my/id/eprint/42047/
https://www.mdpi.com/2075-4450/13/5/466
https://doi.org/10.3390/insects13050466
_version_ 1822896171829428224
score 13.244413

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