Effect of mass transfer in a recirculation batch reactor system for immobilized penicillin amidase
The effect of external mass transfer resistance on the overall reaction rate of the immobilized whole cell penicillin amidase of E. coli in a recirculation batch reactor was investigated. The internal diffusional resistance was found negligible as indicated by the value of effectiveness factor, 0. 9...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
John Wiley & Sons, Inc.
2010
|
| Subjects: | |
| Online Access: | http://dspace.unimap.edu.my/xmlui/handle/123456789/9351 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| id |
my.unimap-9351 |
|---|---|
| record_format |
dspace |
| spelling |
my.unimap-93512010-09-02T08:18:04Z Effect of mass transfer in a recirculation batch reactor system for immobilized penicillin amidase Jong, Moon Park Cheol, Yong Choi Baik, Lin Seong Miyoung, Hyeop Han chemical equipment drug product escherichia coli atc 9637 immobilized penicillin amidase enzymes enzymes technology aminopenicillanic acid penicillin g chemical reaction drug synthesis escherichia coli nonhuman The effect of external mass transfer resistance on the overall reaction rate of the immobilized whole cell penicillin amidase of E. coli in a recirculation batch reactor was investigated. The internal diffusional resistance was found negligible as indicated by the value of effectiveness factor, 0. 95. The local environmental change in a column due to the pH drop was successfully overcome by employing a buffer solution. The reaction rate was measured by the pH-stat method and was found to follow the simple Michaelis-Menten law at the initial stage of the reaction. The values of the net reaction rate experimentally determined were used to calculate the substrate concentration at the external surface of the catalyst pellet and then to calculate the mass transfer coefficient, k//L, at various flow rates and substrate concentrations. 2010-09-02T08:18:04Z 2010-09-02T08:18:04Z 1982-10-10 Article Biotechnology and Bioengineering, vol. 24, no.10, 1982, pages 2215-2226 0006-3592 0006-3592 http://hdl.handle.net/123456789/9351 en John Wiley & Sons, Inc. |
| institution |
Universiti Malaysia Perlis |
| building |
UniMAP Library |
| collection |
Institutional Repository |
| continent |
Asia |
| country |
Malaysia |
| content_provider |
Universiti Malaysia Perlis |
| content_source |
UniMAP Library Digital Repository |
| url_provider |
http://dspace.unimap.edu.my/ |
| language |
English |
| topic |
chemical equipment drug product escherichia coli atc 9637 immobilized penicillin amidase enzymes enzymes technology aminopenicillanic acid penicillin g chemical reaction drug synthesis escherichia coli nonhuman |
| spellingShingle |
chemical equipment drug product escherichia coli atc 9637 immobilized penicillin amidase enzymes enzymes technology aminopenicillanic acid penicillin g chemical reaction drug synthesis escherichia coli nonhuman Jong, Moon Park Cheol, Yong Choi Baik, Lin Seong Miyoung, Hyeop Han Effect of mass transfer in a recirculation batch reactor system for immobilized penicillin amidase |
| description |
The effect of external mass transfer resistance on the overall reaction rate of the immobilized whole cell penicillin amidase of E. coli in a recirculation batch reactor was investigated. The internal diffusional resistance was found negligible as indicated by the value of effectiveness factor, 0. 95. The local environmental change in a column due to the pH drop was successfully overcome by employing a buffer solution. The reaction rate was measured by the pH-stat method and was found to follow the simple Michaelis-Menten law at the initial stage of the reaction. The values of the net reaction rate experimentally determined were used to calculate the substrate concentration at the external surface of the catalyst pellet and then to calculate the mass transfer coefficient, k//L, at various flow rates and substrate concentrations. |
| format |
Article |
| author |
Jong, Moon Park Cheol, Yong Choi Baik, Lin Seong Miyoung, Hyeop Han |
| author_facet |
Jong, Moon Park Cheol, Yong Choi Baik, Lin Seong Miyoung, Hyeop Han |
| author_sort |
Jong, Moon Park |
| title |
Effect of mass transfer in a recirculation batch reactor system for immobilized penicillin amidase |
| title_short |
Effect of mass transfer in a recirculation batch reactor system for immobilized penicillin amidase |
| title_full |
Effect of mass transfer in a recirculation batch reactor system for immobilized penicillin amidase |
| title_fullStr |
Effect of mass transfer in a recirculation batch reactor system for immobilized penicillin amidase |
| title_full_unstemmed |
Effect of mass transfer in a recirculation batch reactor system for immobilized penicillin amidase |
| title_sort |
effect of mass transfer in a recirculation batch reactor system for immobilized penicillin amidase |
| publisher |
John Wiley & Sons, Inc. |
| publishDate |
2010 |
| url |
http://dspace.unimap.edu.my/xmlui/handle/123456789/9351 |
| _version_ |
1643787800263262208 |
| score |
13.222552 |