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Antimicrobial and antioxidant activities from leaves and roots of (Elephantopus Scaber L.) extracted using different solvents

Master of Science in Bioprocess Engineering

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Bibliographic Details
Main Author: Mohammed Riyadh, Mohammed Al-Ani
Other Authors: Mohd Irfan, Mohamad Dzahir, Dr.
Format: Thesis
Language:English
Published: Universiti Malaysia Perlis (UniMAP) 2017
Subjects:
Bioactive compounds
Anti-infective agents
Antioxidants
Ethnobotany
Medicinal plants
Solvents
Online Access:http://dspace.unimap.edu.my:80/xmlui/handle/123456789/78034
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spelling my.unimap-780342023-04-04T02:02:13Z Antimicrobial and antioxidant activities from leaves and roots of (Elephantopus Scaber L.) extracted using different solvents Mohammed Riyadh, Mohammed Al-Ani Mohd Irfan, Mohamad Dzahir, Dr. Bioactive compounds Anti-infective agents Antioxidants Ethnobotany Medicinal plants Solvents Master of Science in Bioprocess Engineering Medicinal plants are used traditionally in treatment of various kinds of diseases since time immemorial. The present study was carried out to evaluate the antimicrobial and antioxidant activities of roots and leaves of Elephantopus scaber by using different solvent (ethyl alcohol ,acetone and water). The ethyl alcohol, acetone and water extracts of the roots and leaves were subjected to phytochemical analyses, which revealed the presence of alkaloids, phenols, flavonoids, saponins, steroids, tannins, coumarins, quinones and glycosides in most of the selected of roots and leaves useing different solvents. Ethyl alcohol, acetone and water extracts of roots and leaves were investigated for in.vitro.antimicrobial activity against Escherichia coli, Salmonella typhis, Klebsiella pneumonia, Streptococcus pyogenes, Staphylococcus aureus, and Candida Albican by well diffusion method using different concentrations (20, 40, 60, 80, 100)mg/ml. Bioassay data obtained from the active extracts and oils were subjected to statistical analysis of variance (ANOVA). Treatment means showing significant difference (p ≤ 0.05) were separated using Student-Newman-Keuls test (SNK). Ethyl alcohol and acetone extract of roots showed promising antimicrobial activity against all test organisms. Ethyl alcohol extract of root gave a significant effect activity against all test organisms compared with the other sample. Ethyl alcohol extract of leaves was also higher compared with the acetone extract of roots for a concentrations of 100 and 20mg/ml. The water extract of leaves has a weak activity against all test organisms and did not inhibit the growth of Klebsiella pneumonia for a concentrations of 100mg/ml but at a concentrations of 20mg/ml did not inhibit the growth of all test organisms. The Minimum inhibitory concentration (MIC) of the roots and leaves extracts by using different solvent were also determined against different test organisms. The MIC value of roots and leaves extract ranged from 100 mg/ml to 500 mg/ml. The ethyl alcohol, acetone and water extract of roots and leaves were selected for possible antimicrobial activity. The antimicrobial activity was studied against six microorganisms namely Escherichia coli, Salmonella typhis, Klebsiella pneumonia, Streptococcus pyogenes, Staphylococcus aureus, and Candida Albican. It was found that ethyl alcohol extract of roots showed height influence against Escherichia coli, Salmonella typhis, Streptococcus pyogenes, Staphylococcus aureus, and Candida Albican but less effective against Klebsiella pneumonia. The water extract showed a weak effect against all tests organisms and did not has any effect against Klebsiella pneumonia. The roots and leaves extract from Elephantopus scaber using different solvents to extract were evaluated for antioxidant activity by using DPPH method using a different concentrations (1, 10, 20, 30, 40, 60, 80, 100) μg/ml. The results from the analysis were compared with ascorbic acid as standard. 2017 2023-03-07T01:49:04Z 2023-03-07T01:49:04Z Thesis http://dspace.unimap.edu.my:80/xmlui/handle/123456789/78034 en Universiti Malaysia Perlis (UniMAP) Universiti Malaysia Perlis (UniMAP) School of Bioprocess Engineering
institution Universiti Malaysia Perlis
building UniMAP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Perlis
content_source UniMAP Library Digital Repository
url_provider http://dspace.unimap.edu.my/
language English
topic Bioactive compounds
Anti-infective agents
Antioxidants
Ethnobotany
Medicinal plants
Solvents
spellingShingle Bioactive compounds
Anti-infective agents
Antioxidants
Ethnobotany
Medicinal plants
Solvents
Mohammed Riyadh, Mohammed Al-Ani
Antimicrobial and antioxidant activities from leaves and roots of (Elephantopus Scaber L.) extracted using different solvents
description Master of Science in Bioprocess Engineering
author2 Mohd Irfan, Mohamad Dzahir, Dr.
author_facet Mohd Irfan, Mohamad Dzahir, Dr.
Mohammed Riyadh, Mohammed Al-Ani
format Thesis
author Mohammed Riyadh, Mohammed Al-Ani
author_sort Mohammed Riyadh, Mohammed Al-Ani
title Antimicrobial and antioxidant activities from leaves and roots of (Elephantopus Scaber L.) extracted using different solvents
title_short Antimicrobial and antioxidant activities from leaves and roots of (Elephantopus Scaber L.) extracted using different solvents
title_full Antimicrobial and antioxidant activities from leaves and roots of (Elephantopus Scaber L.) extracted using different solvents
title_fullStr Antimicrobial and antioxidant activities from leaves and roots of (Elephantopus Scaber L.) extracted using different solvents
title_full_unstemmed Antimicrobial and antioxidant activities from leaves and roots of (Elephantopus Scaber L.) extracted using different solvents
title_sort antimicrobial and antioxidant activities from leaves and roots of (elephantopus scaber l.) extracted using different solvents
publisher Universiti Malaysia Perlis (UniMAP)
publishDate 2017
url http://dspace.unimap.edu.my:80/xmlui/handle/123456789/78034
_version_ 1772813091899179008
score 13.222552

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