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Improvement of Glucose Oxidase Immobilization using Sol-Gel technique

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Main Authors: Nashrul Fazli, Mohd Nasir, Yang, Y.
Other Authors: nashrul@unimap.edu.my
Format: Article
Language:English
Published: Universiti Malaysia Perlis 2010
Subjects:
Biosensor
Glucose sensor
Immobilization technique
Sol gel technique
Hiocompatibility
Online Access:http://dspace.unimap.edu.my/xmlui/handle/123456789/7450
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spelling my.unimap-74502015-07-14T01:34:31Z Improvement of Glucose Oxidase Immobilization using Sol-Gel technique Nashrul Fazli, Mohd Nasir Yang, Y. nashrul@unimap.edu.my Biosensor Glucose sensor Immobilization technique Sol gel technique Hiocompatibility Link to publisher's homepage at http://www.unimap.edu.my/ A sol gel based needle type biosensor using 3-glycidoxypropyl dimethylethoxy silane, as silane agent was developed to improve the immobilisation efficiency of glucose oxidase (GOD). The glucose sensor was coated with 3C-copolymer as outmost layer to increase sensor's bioconipatibility. The biosensor's performance fabricated by this technique was compared with the conventional sensors which uses glntaraldehyde as the cross link agent for immobilisation of GOD. Reliable results were obtained from various characterisation tests, including sensor response to glucose, the sensitivity and stability tests. In the stability test, both types of the sensors were immersed in 20 mM glucose solution for more than 24 hours and the sol gel based sensor showed a better stability. This indicates that the sol gel immobilisation technique applied is able to retain ensyme for longer time of period compared to the conventional method. Furthermore, the studies revealed when the sensors were tested in a glucose solution containing 1 % bovine serum albumin in a long term stability test, the performance of the sol gel based sensor coated with 3C-copolymer was not affected considerably by protein adsorption, which is a vital character for an in vivo biosensor. 2010-01-01T07:27:42Z 2010-01-01T07:27:42Z 2007 Article Journal of Engineering Research and Education, vol. 4, 2007, pages 62-73. 1823-2981 http://www.unimap.edu.my/ http://hdl.handle.net/123456789/7450 en Universiti Malaysia Perlis
institution Universiti Malaysia Perlis
building UniMAP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Perlis
content_source UniMAP Library Digital Repository
url_provider http://dspace.unimap.edu.my/
language English
topic Biosensor
Glucose sensor
Immobilization technique
Sol gel technique
Hiocompatibility
spellingShingle Biosensor
Glucose sensor
Immobilization technique
Sol gel technique
Hiocompatibility
Nashrul Fazli, Mohd Nasir
Yang, Y.
Improvement of Glucose Oxidase Immobilization using Sol-Gel technique
description Link to publisher's homepage at http://www.unimap.edu.my/
author2 nashrul@unimap.edu.my
author_facet nashrul@unimap.edu.my
Nashrul Fazli, Mohd Nasir
Yang, Y.
format Article
author Nashrul Fazli, Mohd Nasir
Yang, Y.
author_sort Nashrul Fazli, Mohd Nasir
title Improvement of Glucose Oxidase Immobilization using Sol-Gel technique
title_short Improvement of Glucose Oxidase Immobilization using Sol-Gel technique
title_full Improvement of Glucose Oxidase Immobilization using Sol-Gel technique
title_fullStr Improvement of Glucose Oxidase Immobilization using Sol-Gel technique
title_full_unstemmed Improvement of Glucose Oxidase Immobilization using Sol-Gel technique
title_sort improvement of glucose oxidase immobilization using sol-gel technique
publisher Universiti Malaysia Perlis
publishDate 2010
url http://dspace.unimap.edu.my/xmlui/handle/123456789/7450
_version_ 1643788830711480320
score 13.222552

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