Optimization of thaumatin extraction by aqueous two-phase system (ATPS) using response surface methodology (RSM)

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Main Authors: A.L., Ahmad, C.J.C., Derek, Mohamed Zulkali, Mohamed Daud
Format: Article
Language:English
Published: Elsevier B.V. 2009
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Online Access:http://dspace.unimap.edu.my/xmlui/handle/123456789/6673
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spelling my.unimap-66732009-08-04T07:43:53Z Optimization of thaumatin extraction by aqueous two-phase system (ATPS) using response surface methodology (RSM) A.L., Ahmad C.J.C., Derek Mohamed Zulkali, Mohamed Daud Thaumatin Aqueous two-phase system Polyethylene glycol/sodium sulfate system Two-level fractional factorial design Response surface methodology Polyethylene glycol Polyethylene glycol -- Biotechnology Separation (Technology) Link to publisher's homepage at www.elsevier.com The work attempts to study and optimize aqueous two-phase system (ATPS) made of polyethylene glycol (PEG)/sodium sulfate and PEG/potassium phosphate. Five factors (PEG's molecular weight, PEG's concentration, pH, concentration of sodium chloride and phase-forming salt) affecting the thaumatin partitioning were studied. A two-level fractional factorial was initially carried out. The statistical analysis showed that for both the systems, the phase-forming salt concentration and the concentration of sodium chloride significantly affects the K value for thaumatin partitioning. However, the performance of PEG/sodium sulfate system was generally better than the PEG/potassium phosphate system. Hence a detail study was carried out on the PEG/sodium sulfate system using central composite rotatable design (CCRD) in response surface methodology (RSM). It was observed that high salt concentration increased the K value by sevenfold and the PEG molecular weight was not a significant factor. The optimal condition gave a K value for thaumatin partitioning of 24.15 with a yield of 96.02%. 2009-08-04T07:43:29Z 2009-08-04T07:43:29Z 2008-09-22 Article Separation and Purification Technology, vol.62 (3), 2008, pages 702-708. 13835866 http://www.sciencedirect.com/science/journal/13835866 http://hdl.handle.net/123456789/6673 en Elsevier B.V.
institution Universiti Malaysia Perlis
building UniMAP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Perlis
content_source UniMAP Library Digital Repository
url_provider http://dspace.unimap.edu.my/
language English
topic Thaumatin
Aqueous two-phase system
Polyethylene glycol/sodium sulfate system
Two-level fractional factorial design
Response surface methodology
Polyethylene glycol
Polyethylene glycol -- Biotechnology
Separation (Technology)
spellingShingle Thaumatin
Aqueous two-phase system
Polyethylene glycol/sodium sulfate system
Two-level fractional factorial design
Response surface methodology
Polyethylene glycol
Polyethylene glycol -- Biotechnology
Separation (Technology)
A.L., Ahmad
C.J.C., Derek
Mohamed Zulkali, Mohamed Daud
Optimization of thaumatin extraction by aqueous two-phase system (ATPS) using response surface methodology (RSM)
description Link to publisher's homepage at www.elsevier.com
format Article
author A.L., Ahmad
C.J.C., Derek
Mohamed Zulkali, Mohamed Daud
author_facet A.L., Ahmad
C.J.C., Derek
Mohamed Zulkali, Mohamed Daud
author_sort A.L., Ahmad
title Optimization of thaumatin extraction by aqueous two-phase system (ATPS) using response surface methodology (RSM)
title_short Optimization of thaumatin extraction by aqueous two-phase system (ATPS) using response surface methodology (RSM)
title_full Optimization of thaumatin extraction by aqueous two-phase system (ATPS) using response surface methodology (RSM)
title_fullStr Optimization of thaumatin extraction by aqueous two-phase system (ATPS) using response surface methodology (RSM)
title_full_unstemmed Optimization of thaumatin extraction by aqueous two-phase system (ATPS) using response surface methodology (RSM)
title_sort optimization of thaumatin extraction by aqueous two-phase system (atps) using response surface methodology (rsm)
publisher Elsevier B.V.
publishDate 2009
url http://dspace.unimap.edu.my/xmlui/handle/123456789/6673
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score 13.222552