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Protein–membrane interactions in forced-flow electrophoresis of protein solutions: effect of initial pH and initial ionic strength

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Main Authors: Ahmad, A.L, Hairul Nazirah, Abdul Halim
Format: Article
Language:English
Published: Elsevier B.V. 2009
Subjects:
Forced-flow electrophoresis
Protein–membrane interactions
Protein
Bovine serum albumin
Bovine γ-globulin
Membrane proteins
Serum albumin
Online Access:http://dspace.unimap.edu.my/xmlui/handle/123456789/6669
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spelling my.unimap-66692009-08-03T08:53:24Z Protein–membrane interactions in forced-flow electrophoresis of protein solutions: effect of initial pH and initial ionic strength Ahmad, A.L Hairul Nazirah, Abdul Halim Forced-flow electrophoresis Protein–membrane interactions Protein Bovine serum albumin Bovine γ-globulin Membrane proteins Serum albumin Link to publisher's homepage at http://www.elsevier.com The purpose of this paper is to determine the effect of initial pH buffer and initial ionic strength of phosphate buffer in forced-flow electrophoresis (FFE) of protein solutions. Two types of proteins, bovine serum albumin (BSA) and bovine γ-globulin (BGG) were used in these experiments. The flux profiles of FFE process were strongly influenced by electrokinetic effects in FFE process. The electrokinetic effects were electrophoresis, electro-osmosis and electrolysis. It was observed that the migration of electrolysis products (H+) changed the pH of retentate which consequently influenced the permeate flux during the FFE process. The lowest permeate flux occurred at the isoelectric point (IEP) of both BSA (pH 4.9) and BGG (pH 5.86–6.70). It was found that the protein–membrane interactions played important role on the permeate flux profile due to electrostatic interaction/repulsion in the FFE process. 2009-08-03T08:53:00Z 2009-08-03T08:53:00Z 2009-04-20 Article Separation and Purification Technology, vol.66 (2), 2009, pages 273-278. 1383-5866 http://www.sciencedirect.com/science/journal/13835866 http://hdl.handle.net/123456789/6669 en Elsevier B.V.
institution Universiti Malaysia Perlis
building UniMAP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Perlis
content_source UniMAP Library Digital Repository
url_provider http://dspace.unimap.edu.my/
language English
topic Forced-flow electrophoresis
Protein–membrane interactions
Protein
Bovine serum albumin
Bovine γ-globulin
Membrane proteins
Serum albumin
spellingShingle Forced-flow electrophoresis
Protein–membrane interactions
Protein
Bovine serum albumin
Bovine γ-globulin
Membrane proteins
Serum albumin
Ahmad, A.L
Hairul Nazirah, Abdul Halim
Protein–membrane interactions in forced-flow electrophoresis of protein solutions: effect of initial pH and initial ionic strength
description Link to publisher's homepage at http://www.elsevier.com
format Article
author Ahmad, A.L
Hairul Nazirah, Abdul Halim
author_facet Ahmad, A.L
Hairul Nazirah, Abdul Halim
author_sort Ahmad, A.L
title Protein–membrane interactions in forced-flow electrophoresis of protein solutions: effect of initial pH and initial ionic strength
title_short Protein–membrane interactions in forced-flow electrophoresis of protein solutions: effect of initial pH and initial ionic strength
title_full Protein–membrane interactions in forced-flow electrophoresis of protein solutions: effect of initial pH and initial ionic strength
title_fullStr Protein–membrane interactions in forced-flow electrophoresis of protein solutions: effect of initial pH and initial ionic strength
title_full_unstemmed Protein–membrane interactions in forced-flow electrophoresis of protein solutions: effect of initial pH and initial ionic strength
title_sort protein–membrane interactions in forced-flow electrophoresis of protein solutions: effect of initial ph and initial ionic strength
publisher Elsevier B.V.
publishDate 2009
url http://dspace.unimap.edu.my/xmlui/handle/123456789/6669
_version_ 1643788536239882240
score 13.211869

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