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Applicability of protease and urea as a molluscicide for Pomacea canaliculata (Siput Gondang Emas)

Pomacea canaliculata is regarded as one of the most destructive molluscs as it causes severe damage to paddy fields. This mollusc is commonly combated by using chemical molluscicide like metaldehyde, which is known as toxic chemicals, therefore causes negative impact to the farmers and the environm...

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Main Author: Noor Hasyierah, Mohd Salleh
Other Authors: Assoc. Prof Dr Dachyar Arbain
Format: Thesis
Language:English
Published: Universiti Malaysia Perlis (UniMAP) 2019
Subjects:
Pomacea canaliculata
Molluscicide
Paddy fields
Environmental-friendly
Pomacea
Online Access:http://dspace.unimap.edu.my:80/xmlui/handle/123456789/61513
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spelling my.unimap-615132023-04-04T02:12:52Z Applicability of protease and urea as a molluscicide for Pomacea canaliculata (Siput Gondang Emas) Noor Hasyierah, Mohd Salleh Assoc. Prof Dr Dachyar Arbain Pomacea canaliculata Molluscicide Paddy fields Environmental-friendly Pomacea Pomacea canaliculata is regarded as one of the most destructive molluscs as it causes severe damage to paddy fields. This mollusc is commonly combated by using chemical molluscicide like metaldehyde, which is known as toxic chemicals, therefore causes negative impact to the farmers and the environment. Hence, there is a great demand for a user and environmental-friendly molluscicide to reduce P. canaliculata population. The present study investigates the applicability of protease and urea as an alternative molluscicide for combating both flesh and eggs of P. canaliculata. Studies on the effect of each agent on snail eggs cover chemical and physical analyses; namely unhatchability, cuticle protein profile, water loss, conductivity, gas exchange and morphological changes. For snail flesh, it covers biological, chemical and physical studies, namely, mortality, respiration, food intake, responsiveness, movement, protein, ammonia and peroxidase level of the flesh. The studies were extended by producing cell free extract protease (CFE protease) using locally available raw material like cassava, and subsequently applied as a biomolluscicide. Likewise, studies regarding urea were also extended by using coated urea with rice husk ash and starch before subsequently applied to the paddy demonstration plot. For the flesh of P. canaliculata studies, it was found that the mortality were 31% and 90% after exposure to 3.9U/mL CFE protease and 0.02M urea respectively for 20hrs. It was also observed that the respiration rate increased proportionally with the increase of urea concentration. Additionally, the exposure to CFE protease and urea also reduced the snail appetite, responsiveness and movement as compared to the control snail. Extension of the work using coated urea for paddy plot studies saved 93% of paddy from snail attack. As for the P. canaliculata eggs studies, 71%, 52% and 35% hatchability have been suppressed after treating to 0.75U/mL commercial protease, 0.19U/mL CFE protease and 0.02M urea respectively. Besides that, the rate of conductivity, water loss and respiration increased proportionally with the increase of either commercial protease activity or urea concentration. The findings from these studies show the applicability of protease and urea as a molluscicide for both the flesh and eggs of P. canaliculata. 2019-08-21T04:22:38Z 2019-08-21T04:22:38Z 2015 Thesis http://dspace.unimap.edu.my:80/xmlui/handle/123456789/61513 en Universiti Malaysia Perlis (UniMAP) School of Bioprocess Engineering
institution Universiti Malaysia Perlis
building UniMAP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Perlis
content_source UniMAP Library Digital Repository
url_provider http://dspace.unimap.edu.my/
language English
topic Pomacea canaliculata
Molluscicide
Paddy fields
Environmental-friendly
Pomacea
spellingShingle Pomacea canaliculata
Molluscicide
Paddy fields
Environmental-friendly
Pomacea
Noor Hasyierah, Mohd Salleh
Applicability of protease and urea as a molluscicide for Pomacea canaliculata (Siput Gondang Emas)
description Pomacea canaliculata is regarded as one of the most destructive molluscs as it causes severe damage to paddy fields. This mollusc is commonly combated by using chemical molluscicide like metaldehyde, which is known as toxic chemicals, therefore causes negative impact to the farmers and the environment. Hence, there is a great demand for a user and environmental-friendly molluscicide to reduce P. canaliculata population. The present study investigates the applicability of protease and urea as an alternative molluscicide for combating both flesh and eggs of P. canaliculata. Studies on the effect of each agent on snail eggs cover chemical and physical analyses; namely unhatchability, cuticle protein profile, water loss, conductivity, gas exchange and morphological changes. For snail flesh, it covers biological, chemical and physical studies, namely, mortality, respiration, food intake, responsiveness, movement, protein, ammonia and peroxidase level of the flesh. The studies were extended by producing cell free extract protease (CFE protease) using locally available raw material like cassava, and subsequently applied as a biomolluscicide. Likewise, studies regarding urea were also extended by using coated urea with rice husk ash and starch before subsequently applied to the paddy demonstration plot. For the flesh of P. canaliculata studies, it was found that the mortality were 31% and 90% after exposure to 3.9U/mL CFE protease and 0.02M urea respectively for 20hrs. It was also observed that the respiration rate increased proportionally with the increase of urea concentration. Additionally, the exposure to CFE protease and urea also reduced the snail appetite, responsiveness and movement as compared to the control snail. Extension of the work using coated urea for paddy plot studies saved 93% of paddy from snail attack. As for the P. canaliculata eggs studies, 71%, 52% and 35% hatchability have been suppressed after treating to 0.75U/mL commercial protease, 0.19U/mL CFE protease and 0.02M urea respectively. Besides that, the rate of conductivity, water loss and respiration increased proportionally with the increase of either commercial protease activity or urea concentration. The findings from these studies show the applicability of protease and urea as a molluscicide for both the flesh and eggs of P. canaliculata.
author2 Assoc. Prof Dr Dachyar Arbain
author_facet Assoc. Prof Dr Dachyar Arbain
Noor Hasyierah, Mohd Salleh
format Thesis
author Noor Hasyierah, Mohd Salleh
author_sort Noor Hasyierah, Mohd Salleh
title Applicability of protease and urea as a molluscicide for Pomacea canaliculata (Siput Gondang Emas)
title_short Applicability of protease and urea as a molluscicide for Pomacea canaliculata (Siput Gondang Emas)
title_full Applicability of protease and urea as a molluscicide for Pomacea canaliculata (Siput Gondang Emas)
title_fullStr Applicability of protease and urea as a molluscicide for Pomacea canaliculata (Siput Gondang Emas)
title_full_unstemmed Applicability of protease and urea as a molluscicide for Pomacea canaliculata (Siput Gondang Emas)
title_sort applicability of protease and urea as a molluscicide for pomacea canaliculata (siput gondang emas)
publisher Universiti Malaysia Perlis (UniMAP)
publishDate 2019
url http://dspace.unimap.edu.my:80/xmlui/handle/123456789/61513
_version_ 1772813059641835520
score 13.222552

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