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Growth kinetics of a diesel-degrading bacterial strain from petroleum-contaminated soil

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Main Authors: Farrah Aini, Dahalan, Ibrahim, Yunus, Wan Lutfi, Wan Johari, Mohd Yunus, Shukor, Mohd Izuan Effendi, Halmi, Nor Aripin, Shamaan, Mohd Arif, Syed
Other Authors: farrahaini@unimap.edu.my
Format: Article
Language:English
Published: Triveni Enterprises 2016
Subjects:
Acinetobacter sp
Characterization
Diesel-degrading
Haldane
Isolation
Online Access:http://dspace.unimap.edu.my:80/xmlui/handle/123456789/41168
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spelling my.unimap-411682016-03-21T08:57:53Z Growth kinetics of a diesel-degrading bacterial strain from petroleum-contaminated soil Farrah Aini, Dahalan Ibrahim, Yunus Wan Lutfi, Wan Johari Mohd Yunus, Shukor Mohd Izuan Effendi, Halmi Nor Aripin, Shamaan Mohd Arif, Syed farrahaini@unimap.edu.my yunus@biotech.upm.edu.my Acinetobacter sp Characterization Diesel-degrading Haldane Isolation Link to publisher's homepage at http://www.jeb.co.in A diesel-degrading bacterium was isolated from a diesel-contaminated site in Selangor, Malaysia. The isolate was tentatively identified as Acinetobacter sp. strain DRY12 based on partial 16S rDNA molecular phylogeny and Biolog® GN microplate panels and Microlog® database. Optimum growth occurred from 3 to 5% diesel and the strain was able to tolerate as high as 8% diesel. The optimal pH that supported growth of the bacterium was between pH 7.5 to 8.0. The isolate exhibited optimal growth in between 30 and 35° C. The best nitrogen source was potassium nitrate (between 0.6 and 0.9% (w/v)) followed by ammonium chloride, sodium nitrite and ammonium sulphate in descending order. An almost complete removal of diesel components was seen from the reduction in hydrocarbon peaks observed using Solid Phase Microextraction Gas Chromatography analysis after 10 days of incubation. The best growth kinetic model to fit experimental data was the Haldane model of substrate inhibiting growth with a correlation coefficient value of 0.97. The maximum growth rate- μmax was 0.039 hr -1 while the saturation constant or half velocity constant Ks and inhibition constant Ki, were 0.387% and 4.46%, respectively. MATH assays showed that 75% of the bacterium was found in the hexadecane phase indicating that the bacterium was hydrophobic. The characteristics of this bacterium make it useful for bioremediation works in the Tropics. 2016-03-21T08:56:36Z 2016-03-21T08:56:36Z 2014-03 Article Journal of Environmental Biology, vol.35 (2), 2014, pages 399-406 0254-8704 http://www.jeb.co.in/journal_issues/201403_mar14/paper_16.pdf http://dspace.unimap.edu.my:80/xmlui/handle/123456789/41168 en Triveni Enterprises
institution Universiti Malaysia Perlis
building UniMAP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Perlis
content_source UniMAP Library Digital Repository
url_provider http://dspace.unimap.edu.my/
language English
topic Acinetobacter sp
Characterization
Diesel-degrading
Haldane
Isolation
spellingShingle Acinetobacter sp
Characterization
Diesel-degrading
Haldane
Isolation
Farrah Aini, Dahalan
Ibrahim, Yunus
Wan Lutfi, Wan Johari
Mohd Yunus, Shukor
Mohd Izuan Effendi, Halmi
Nor Aripin, Shamaan
Mohd Arif, Syed
Growth kinetics of a diesel-degrading bacterial strain from petroleum-contaminated soil
description Link to publisher's homepage at http://www.jeb.co.in
author2 farrahaini@unimap.edu.my
author_facet farrahaini@unimap.edu.my
Farrah Aini, Dahalan
Ibrahim, Yunus
Wan Lutfi, Wan Johari
Mohd Yunus, Shukor
Mohd Izuan Effendi, Halmi
Nor Aripin, Shamaan
Mohd Arif, Syed
format Article
author Farrah Aini, Dahalan
Ibrahim, Yunus
Wan Lutfi, Wan Johari
Mohd Yunus, Shukor
Mohd Izuan Effendi, Halmi
Nor Aripin, Shamaan
Mohd Arif, Syed
author_sort Farrah Aini, Dahalan
title Growth kinetics of a diesel-degrading bacterial strain from petroleum-contaminated soil
title_short Growth kinetics of a diesel-degrading bacterial strain from petroleum-contaminated soil
title_full Growth kinetics of a diesel-degrading bacterial strain from petroleum-contaminated soil
title_fullStr Growth kinetics of a diesel-degrading bacterial strain from petroleum-contaminated soil
title_full_unstemmed Growth kinetics of a diesel-degrading bacterial strain from petroleum-contaminated soil
title_sort growth kinetics of a diesel-degrading bacterial strain from petroleum-contaminated soil
publisher Triveni Enterprises
publishDate 2016
url http://dspace.unimap.edu.my:80/xmlui/handle/123456789/41168
_version_ 1643799598133673984
score 13.211869

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