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Purification and kinetic study of protease enzyme from kesinai (streblus asper) leave

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Bibliographic Details
Main Author: Nurul Izzati Izni, Mat Yusoff
Other Authors: Rozaini Abdullah
Format: Learning Object
Language:English
Published: Universiti Malaysia Perlis (UniMAP) 2014
Subjects:
Kesinai (Streblus asper)
Kinetic study
Protease enzyme
Extraction
Online Access:http://dspace.unimap.edu.my:80/xmlui/handle/123456789/37730
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spelling my.unimap-377302014-11-07T10:06:34Z Purification and kinetic study of protease enzyme from kesinai (streblus asper) leave Nurul Izzati Izni, Mat Yusoff Rozaini Abdullah Kesinai (Streblus asper) Kinetic study Protease enzyme Extraction Access is limited to UniMAP community. A study was carried out to purify protease enzyme from kesinai leaves extract. The protease enzyme was extracted by using phosphate buffer at pH 7.5. The crude enzyme extract was further with the purification technique by using heat-treatment aqueous two phase system with ratio (w/w) of 2:4.5:1.6:1.5:0.4 crude enzymes, distilled water, PEG, MgSO4 and NaCl accordingly. The separation achieved after centrifugation and was proceed to freeze dry. The observation on enzyme extracted was done and found to have change in color as it was purified. The absorbance of the enzyme was measured by using SHIMADZU spectrophotometer to calculate the enzyme activity and protein concentration. The enzyme activity was increase gradually after being purified and freeze dry with 6.17 and 6.96 U/mL accordingly. The protein concentration for the enzyme decrease dramatically after being purify and freeze dry with 526 and 411 mg/mL. The recovery of protease enzyme was enhanced after freeze dry with purification fold 19.03 and percent yield of 31.1 %. The study was further by investigating kinetic study of sample before and after freeze dry. The VMax and KM value for sample before freeze dry were 0.0324 s-1 and 0.1243 M while for sample after freeze dry were 0.0309 s-1 and 0.0371 M accordingly. The results show that the protease enzyme after freeze drying had a higher affinity for the substrate and higher proteolytic activity than the protease before freeze dry. 2014-11-07T10:06:34Z 2014-11-07T10:06:34Z 2013-06 Learning Object http://dspace.unimap.edu.my:80/xmlui/handle/123456789/37730 en Universiti Malaysia Perlis (UniMAP) School of Bioprocess Engineering
institution Universiti Malaysia Perlis
building UniMAP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Perlis
content_source UniMAP Library Digital Repository
url_provider http://dspace.unimap.edu.my/
language English
topic Kesinai (Streblus asper)
Kinetic study
Protease enzyme
Extraction
spellingShingle Kesinai (Streblus asper)
Kinetic study
Protease enzyme
Extraction
Nurul Izzati Izni, Mat Yusoff
Purification and kinetic study of protease enzyme from kesinai (streblus asper) leave
description Access is limited to UniMAP community.
author2 Rozaini Abdullah
author_facet Rozaini Abdullah
Nurul Izzati Izni, Mat Yusoff
format Learning Object
author Nurul Izzati Izni, Mat Yusoff
author_sort Nurul Izzati Izni, Mat Yusoff
title Purification and kinetic study of protease enzyme from kesinai (streblus asper) leave
title_short Purification and kinetic study of protease enzyme from kesinai (streblus asper) leave
title_full Purification and kinetic study of protease enzyme from kesinai (streblus asper) leave
title_fullStr Purification and kinetic study of protease enzyme from kesinai (streblus asper) leave
title_full_unstemmed Purification and kinetic study of protease enzyme from kesinai (streblus asper) leave
title_sort purification and kinetic study of protease enzyme from kesinai (streblus asper) leave
publisher Universiti Malaysia Perlis (UniMAP)
publishDate 2014
url http://dspace.unimap.edu.my:80/xmlui/handle/123456789/37730
_version_ 1643798535351566336
score 13.211869

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