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Optimization of process conditions of lipase production from coconut dregs by solid state fermentation

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Bibliographic Details
Main Author: Nittiyah, Vasudevan
Other Authors: Mohamad Fahrurrazi Tompang
Format: Learning Object
Language:English
Published: Universiti Malaysia Perlis (UniMAP) 2014
Subjects:
Lipases
Solid state fermentation (SSF)
Coconut dregs
Lipase production
Online Access:http://dspace.unimap.edu.my:80/xmlui/handle/123456789/37625
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spelling my.unimap-376252014-11-05T02:12:00Z Optimization of process conditions of lipase production from coconut dregs by solid state fermentation Nittiyah, Vasudevan Mohamad Fahrurrazi Tompang Lipases Solid state fermentation (SSF) Coconut dregs Lipase production Access is limited to UniMAP community. Lipases comprise a group of hydrolytic enzymes which catalyze the hydrolysis and synthesis of triacylglycerides in the oil water interface. Screening of microorganism for extracellular lipase production from coconut dregs was conducted by using Aspergillus niger and Rhizopus oligosporus. The result shows that Rhizopus oligosporus able to produce higher lipase, 0.1527 U/mL compared to Aspergillus niger and thus it was chosen for further analysis. Process conditions such as pH, temperature and moisture content which influence the growth and production of lipase were studied. Central-Composite Design (CCD) of Response Surface approach was used to analyze the results and the optimum conditions for lipase production was determined. According to CCD, the optimum process conditions for Rhizopus oligosporus to produce lipase were as follow: 34.8 ˚C, pH 5.24 and 62 % of moisture content. Validation process was carried out for 10 days under these conditions and it gives the maximum lipase activity at day 6 (0.2281 U/mL) . The standard error between optimization and validation was 2.23 %. 2014-11-05T02:12:00Z 2014-11-05T02:12:00Z 2013-05 Learning Object http://dspace.unimap.edu.my:80/xmlui/handle/123456789/37625 en Universiti Malaysia Perlis (UniMAP) School of Bioprocess Engineering
institution Universiti Malaysia Perlis
building UniMAP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Perlis
content_source UniMAP Library Digital Repository
url_provider http://dspace.unimap.edu.my/
language English
topic Lipases
Solid state fermentation (SSF)
Coconut dregs
Lipase production
spellingShingle Lipases
Solid state fermentation (SSF)
Coconut dregs
Lipase production
Nittiyah, Vasudevan
Optimization of process conditions of lipase production from coconut dregs by solid state fermentation
description Access is limited to UniMAP community.
author2 Mohamad Fahrurrazi Tompang
author_facet Mohamad Fahrurrazi Tompang
Nittiyah, Vasudevan
format Learning Object
author Nittiyah, Vasudevan
author_sort Nittiyah, Vasudevan
title Optimization of process conditions of lipase production from coconut dregs by solid state fermentation
title_short Optimization of process conditions of lipase production from coconut dregs by solid state fermentation
title_full Optimization of process conditions of lipase production from coconut dregs by solid state fermentation
title_fullStr Optimization of process conditions of lipase production from coconut dregs by solid state fermentation
title_full_unstemmed Optimization of process conditions of lipase production from coconut dregs by solid state fermentation
title_sort optimization of process conditions of lipase production from coconut dregs by solid state fermentation
publisher Universiti Malaysia Perlis (UniMAP)
publishDate 2014
url http://dspace.unimap.edu.my:80/xmlui/handle/123456789/37625
_version_ 1643798525360734208
score 13.222552

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