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Comparative study on human and bovine AT-SC isolation methods

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Main Authors: Ali Hussain, Reshak, Prof. Dr., Mukhzeer, Mohamad Shahimin, Dr., Fhataheya, Buang
Other Authors: maalidph@yahoo.co.uk
Format: Article
Language:English
Published: Elsevier Ltd. 2014
Subjects:
Abdominal subcutaneous adipose tissue
Bovine
Human
Stem cell isolation
Online Access:http://dspace.unimap.edu.my:80/dspace/handle/123456789/35114
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spelling my.unimap-351142014-06-05T06:47:11Z Comparative study on human and bovine AT-SC isolation methods Ali Hussain, Reshak, Prof. Dr. Mukhzeer, Mohamad Shahimin, Dr. Fhataheya, Buang maalidph@yahoo.co.uk mukhzeer@unimap.edu.my  fhataheya_buang@yahoo.com Abdominal subcutaneous adipose tissue Bovine Human Stem cell isolation Link to publisher's homepage at http://www.elsevier.com/ Mammalian adipose tissue derived stem cells (AT-SC) have a tremendous potential in regenerative medicine for tissue engineering and somatic nuclear transfer (SNT). The isolation methods of human and bovine adipose tissue derived stem cells are compared in this paper to determine the feasibility and optimum method of isolation. The optimum isolation method will reduce the processing time, efforts and money as isolation is the first crucial and important step in stem cells research. Human abdominal subcutaneous adipose tissue and bovine abdominal subcutaneous adipose tissue are digested in three collagenase type 1 concentration 0.075%, 0.3% and 0.6% agitated at 1h and 2h under 37°C in 5% CO2 incubator. The cultures are then morphologically characterised. Human adipose tissue stem cells are found to be best isolated using abdominal subcutaneous depot, using 0.075% collagenase type 1 agitated at 1h under 37°C in CO2 incubator. While bovine adipose tissue derived stem cells are best isolated using abdominal subcutaneous depot, using 0.6% collagenase type 1 agitated at 2h under 37°C in CO2 incubator. 2014-06-05T06:47:11Z 2014-06-05T06:47:11Z 2013-11 Article Progress in Biophysics and Molecular Biology, vol. 113(2), 2013, pages 295-298 0079-6107 http://www.sciencedirect.com/science/article/pii/S007961071300093X http://dspace.unimap.edu.my:80/dspace/handle/123456789/35114 en Elsevier Ltd.
institution Universiti Malaysia Perlis
building UniMAP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Perlis
content_source UniMAP Library Digital Repository
url_provider http://dspace.unimap.edu.my/
language English
topic Abdominal subcutaneous adipose tissue
Bovine
Human
Stem cell isolation
spellingShingle Abdominal subcutaneous adipose tissue
Bovine
Human
Stem cell isolation
Ali Hussain, Reshak, Prof. Dr.
Mukhzeer, Mohamad Shahimin, Dr.
Fhataheya, Buang
Comparative study on human and bovine AT-SC isolation methods
description Link to publisher's homepage at http://www.elsevier.com/
author2 maalidph@yahoo.co.uk
author_facet maalidph@yahoo.co.uk
Ali Hussain, Reshak, Prof. Dr.
Mukhzeer, Mohamad Shahimin, Dr.
Fhataheya, Buang
format Article
author Ali Hussain, Reshak, Prof. Dr.
Mukhzeer, Mohamad Shahimin, Dr.
Fhataheya, Buang
author_sort Ali Hussain, Reshak, Prof. Dr.
title Comparative study on human and bovine AT-SC isolation methods
title_short Comparative study on human and bovine AT-SC isolation methods
title_full Comparative study on human and bovine AT-SC isolation methods
title_fullStr Comparative study on human and bovine AT-SC isolation methods
title_full_unstemmed Comparative study on human and bovine AT-SC isolation methods
title_sort comparative study on human and bovine at-sc isolation methods
publisher Elsevier Ltd.
publishDate 2014
url http://dspace.unimap.edu.my:80/dspace/handle/123456789/35114
_version_ 1643797710482964480
score 13.222552

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