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DNA hybridization detection using less than 10-nm gap silicon nanogap structure

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Main Authors: Md Eaqub, Ali, Dr., Dhahi, Th Sabar, Rasel, Das, Uda, Hashim, Prof. Dr.
Other Authors: eaqubali@um.edu.my
Format: Article
Language:English
Published: Elsevier B.V. 2014
Subjects:
Discriminating detection
Electrical detection
Enzymatic treatment
Metalization chemistry
SNP genotyping
Online Access:http://dspace.unimap.edu.my:80/dspace/handle/123456789/32626
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spelling my.unimap-326262014-03-12T08:13:08Z DNA hybridization detection using less than 10-nm gap silicon nanogap structure Md Eaqub, Ali, Dr. Dhahi, Th Sabar Rasel, Das Uda, Hashim, Prof. Dr. eaqubali@um.edu.my sthikra@yahoo.com uda@unimap.edu.my Discriminating detection Electrical detection Enzymatic treatment Metalization chemistry SNP genotyping Link to publisher's homepage at http://www.elsevier.com/ This report described less than 10-nm gap silicon-based electrode sensor for the discriminating detection of complementary, non-complementary and single nucleotide mismatched DNA targets. Discriminating detection of nucleic acid sequences is a key step in biodiagnostics, drug discovery, gene expression profiling, environmental monitoring and forensic studies. The method is based on the measurement of capacitance, conductance and permittivity profiles of the nanogap electrodes upon target oligonucleotide hybridization using a simple dielectric analyzer. The nanogap surface was at first modified with a self-assembled layer of amino functionalities followed by non-covalent adsorption of gold nanoparticles to create an attachment surface for the thiol-modified DNA probe. The capacitance, conductance and permittivity profiles of the biosensor clearly differentiated complementary, non-complementary and single mismatch target hybridization without the need of any labeling steps, enzymatic treatment or metalization chemistry. The detection limit and range of the silicon nanogap biosensor were 3 nmol/L and 3-15 nmol/L of target DNA. 2014-03-12T08:13:08Z 2014-03-12T08:13:08Z 2013-09 Article Sensors and Actuators, A: Physical, vol. 199, 2013, pages 304-309 0924-4247 http://www.sciencedirect.com/science/article/pii/S0924424713002938 http://dspace.unimap.edu.my:80/dspace/handle/123456789/32626 en Elsevier B.V.
institution Universiti Malaysia Perlis
building UniMAP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Perlis
content_source UniMAP Library Digital Repository
url_provider http://dspace.unimap.edu.my/
language English
topic Discriminating detection
Electrical detection
Enzymatic treatment
Metalization chemistry
SNP genotyping
spellingShingle Discriminating detection
Electrical detection
Enzymatic treatment
Metalization chemistry
SNP genotyping
Md Eaqub, Ali, Dr.
Dhahi, Th Sabar
Rasel, Das
Uda, Hashim, Prof. Dr.
DNA hybridization detection using less than 10-nm gap silicon nanogap structure
description Link to publisher's homepage at http://www.elsevier.com/
author2 eaqubali@um.edu.my
author_facet eaqubali@um.edu.my
Md Eaqub, Ali, Dr.
Dhahi, Th Sabar
Rasel, Das
Uda, Hashim, Prof. Dr.
format Article
author Md Eaqub, Ali, Dr.
Dhahi, Th Sabar
Rasel, Das
Uda, Hashim, Prof. Dr.
author_sort Md Eaqub, Ali, Dr.
title DNA hybridization detection using less than 10-nm gap silicon nanogap structure
title_short DNA hybridization detection using less than 10-nm gap silicon nanogap structure
title_full DNA hybridization detection using less than 10-nm gap silicon nanogap structure
title_fullStr DNA hybridization detection using less than 10-nm gap silicon nanogap structure
title_full_unstemmed DNA hybridization detection using less than 10-nm gap silicon nanogap structure
title_sort dna hybridization detection using less than 10-nm gap silicon nanogap structure
publisher Elsevier B.V.
publishDate 2014
url http://dspace.unimap.edu.my:80/dspace/handle/123456789/32626
_version_ 1643796936903360512
score 13.211869

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