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Highly sensitive detection of platelet-derived growth factor on a functionalized diamond surface using aptamer sandwich design

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Main Authors: Ruslinda, Abdul Rahim, Dr., Varun, Penmatsa, Yoko, Ishii, Shinya, Tajima, Hiroshi, Kawarada, Prof. Dr.
Other Authors: ruslinda@toki.waseda.jp
Format: Article
Language:English
Published: The Royal Society of Chemistry 2014
Subjects:
Aptamer
Platelet derived growth factor (PDGF)
Diamond
Online Access:http://dspace.unimap.edu.my:80/dspace/handle/123456789/31501
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spelling my.unimap-315012014-01-27T07:05:41Z Highly sensitive detection of platelet-derived growth factor on a functionalized diamond surface using aptamer sandwich design Ruslinda, Abdul Rahim, Dr. Varun, Penmatsa Yoko, Ishii Shinya, Tajima Hiroshi, Kawarada, Prof. Dr. ruslinda@toki.waseda.jp ruslinda@unimap.edu.my kawarada@waseda.jp Aptamer Platelet derived growth factor (PDGF) Diamond Link to publisher's homepage at http://www.rsc.org Aptamer-based fluorescence detection of platelet-derived growth factor (PDGF) on a functionalized diamond surface was demonstrated. In this work, a sandwich design based on the ability of PDGF to bind with aptamers at its two available binding sites was employed. It was found that this sandwich design approach significantly increases the fluorescence signal intensity, and thereby a very low detection limit of 4 pM was achieved. The effect of the ionic strength of MgCl 2 buffer solution was also investigated, and the most favourable binding for PDGF-BB occurred at a Mg 2+ concentration of 5.5 mM. Since the aptamers bind to the target PDGF with high affinity, fluorescence detection exhibited high selectivity towards different biomolecules. The high reproducibility of detection was confirmed by performing three cycles of measurements over a period of three days. 2014-01-27T04:58:33Z 2014-01-27T04:58:33Z 2012-04 Article Analyst, vol. 137(7), 2012, pages 1692-1697 0003-2654 http://pubs.rsc.org/en/Content/ArticleLanding/2012/AN/c2an15933c#!divAbstract http://dspace.unimap.edu.my:80/dspace/handle/123456789/31501 en The Royal Society of Chemistry
institution Universiti Malaysia Perlis
building UniMAP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Perlis
content_source UniMAP Library Digital Repository
url_provider http://dspace.unimap.edu.my/
language English
topic Aptamer
Platelet derived growth factor (PDGF)
Diamond
spellingShingle Aptamer
Platelet derived growth factor (PDGF)
Diamond
Ruslinda, Abdul Rahim, Dr.
Varun, Penmatsa
Yoko, Ishii
Shinya, Tajima
Hiroshi, Kawarada, Prof. Dr.
Highly sensitive detection of platelet-derived growth factor on a functionalized diamond surface using aptamer sandwich design
description Link to publisher's homepage at http://www.rsc.org
author2 ruslinda@toki.waseda.jp
author_facet ruslinda@toki.waseda.jp
Ruslinda, Abdul Rahim, Dr.
Varun, Penmatsa
Yoko, Ishii
Shinya, Tajima
Hiroshi, Kawarada, Prof. Dr.
format Article
author Ruslinda, Abdul Rahim, Dr.
Varun, Penmatsa
Yoko, Ishii
Shinya, Tajima
Hiroshi, Kawarada, Prof. Dr.
author_sort Ruslinda, Abdul Rahim, Dr.
title Highly sensitive detection of platelet-derived growth factor on a functionalized diamond surface using aptamer sandwich design
title_short Highly sensitive detection of platelet-derived growth factor on a functionalized diamond surface using aptamer sandwich design
title_full Highly sensitive detection of platelet-derived growth factor on a functionalized diamond surface using aptamer sandwich design
title_fullStr Highly sensitive detection of platelet-derived growth factor on a functionalized diamond surface using aptamer sandwich design
title_full_unstemmed Highly sensitive detection of platelet-derived growth factor on a functionalized diamond surface using aptamer sandwich design
title_sort highly sensitive detection of platelet-derived growth factor on a functionalized diamond surface using aptamer sandwich design
publisher The Royal Society of Chemistry
publishDate 2014
url http://dspace.unimap.edu.my:80/dspace/handle/123456789/31501
_version_ 1643796577790197760
score 13.222552

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