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Bovine adipose tissue derived stem cells bioengineering: isolation, characterization and differentiation studies

There is a significant potential for stem cells to be exploited for regenerative medicine. Stem cells act as a repair system for the body by differentiating into specialised cells and replenishing cells in regenerative organs such as skin or intestinal tissues. Conventionally in the current cell the...

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Main Author: Fhataheya, Buang
Format: Thesis
Language:English
Published: Universiti Malaysia Perlis (UniMAP) 2014
Subjects:
Stem cells
Regenerative medicine
Bioengineering
Stem cell isolation
Bovine
Human
Stem cells > Research
Online Access:http://dspace.unimap.edu.my:80/dspace/handle/123456789/31295
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spelling my.unimap-312952014-01-19T06:28:22Z Bovine adipose tissue derived stem cells bioengineering: isolation, characterization and differentiation studies Fhataheya, Buang Stem cells Regenerative medicine Bioengineering Stem cell isolation Bovine Human Stem cells --Research There is a significant potential for stem cells to be exploited for regenerative medicine. Stem cells act as a repair system for the body by differentiating into specialised cells and replenishing cells in regenerative organs such as skin or intestinal tissues. Conventionally in the current cell therapy, the renewed, differentiated cells were mostly sourced form bone marrow mesenchymal stem cell (MSCs) yielding low number of cells and involved a painful procedure under general anaesthesia. In tissue engineering, bioprocessing stem cells are crucial thus, stem cells from adipose tissue were revealed as a new promising source of MSCs. Abundant source of stromal vascular fraction (SVF) needs a good bioprocessing tools for an optimize culture. Isolation of bovine adipose tissue derived stem cells (ASCs) are found to better perform in 0.075% collagenase Type 1 and agitated for 2 hours from the experiment carried out. The static cultures were best inoculated at 1.0 x 104 cells/ml in 6 well plate. The suspension cultures were cultured best in 200rpm, 5ml DMEM: F12 / MesenPRO RS™ at 1.0 x 104 cells/ml in 50ml tube. Suspension (3D) culture of sphere aggregates yield cells density of 1.07 x 106 ASCs upon culture compared to static (2D) culture of 5 x 105 ASCs on average. However the viability of static culture are found to be more superior to suspension culture at high volume, agitation and inoculation sizes suggesting that the parameter are an unfavourable environment to culture in 50ml tubes. Successful differentiation of osteoblast cells were evaluated via Fourier Transform Infra-Red (FTIR) and conventional Van Kossa staining. Simulation of suspension (3D) culture via Computational Fluid Dynamic (CFD) using Volume of Fluid (VOF) model assist in developing an optimised ASCs culture protocol vis-à-vis conventional static (2D) culturing in flask. 2014-01-19T06:28:22Z 2014-01-19T06:28:22Z 2013 Thesis http://dspace.unimap.edu.my:80/dspace/handle/123456789/31295 en Universiti Malaysia Perlis (UniMAP) School of Bioprocess Engineering
institution Universiti Malaysia Perlis
building UniMAP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Perlis
content_source UniMAP Library Digital Repository
url_provider http://dspace.unimap.edu.my/
language English
topic Stem cells
Regenerative medicine
Bioengineering
Stem cell isolation
Bovine
Human
Stem cells --Research
spellingShingle Stem cells
Regenerative medicine
Bioengineering
Stem cell isolation
Bovine
Human
Stem cells --Research
Fhataheya, Buang
Bovine adipose tissue derived stem cells bioengineering: isolation, characterization and differentiation studies
description There is a significant potential for stem cells to be exploited for regenerative medicine. Stem cells act as a repair system for the body by differentiating into specialised cells and replenishing cells in regenerative organs such as skin or intestinal tissues. Conventionally in the current cell therapy, the renewed, differentiated cells were mostly sourced form bone marrow mesenchymal stem cell (MSCs) yielding low number of cells and involved a painful procedure under general anaesthesia. In tissue engineering, bioprocessing stem cells are crucial thus, stem cells from adipose tissue were revealed as a new promising source of MSCs. Abundant source of stromal vascular fraction (SVF) needs a good bioprocessing tools for an optimize culture. Isolation of bovine adipose tissue derived stem cells (ASCs) are found to better perform in 0.075% collagenase Type 1 and agitated for 2 hours from the experiment carried out. The static cultures were best inoculated at 1.0 x 104 cells/ml in 6 well plate. The suspension cultures were cultured best in 200rpm, 5ml DMEM: F12 / MesenPRO RS™ at 1.0 x 104 cells/ml in 50ml tube. Suspension (3D) culture of sphere aggregates yield cells density of 1.07 x 106 ASCs upon culture compared to static (2D) culture of 5 x 105 ASCs on average. However the viability of static culture are found to be more superior to suspension culture at high volume, agitation and inoculation sizes suggesting that the parameter are an unfavourable environment to culture in 50ml tubes. Successful differentiation of osteoblast cells were evaluated via Fourier Transform Infra-Red (FTIR) and conventional Van Kossa staining. Simulation of suspension (3D) culture via Computational Fluid Dynamic (CFD) using Volume of Fluid (VOF) model assist in developing an optimised ASCs culture protocol vis-à-vis conventional static (2D) culturing in flask.
format Thesis
author Fhataheya, Buang
author_facet Fhataheya, Buang
author_sort Fhataheya, Buang
title Bovine adipose tissue derived stem cells bioengineering: isolation, characterization and differentiation studies
title_short Bovine adipose tissue derived stem cells bioengineering: isolation, characterization and differentiation studies
title_full Bovine adipose tissue derived stem cells bioengineering: isolation, characterization and differentiation studies
title_fullStr Bovine adipose tissue derived stem cells bioengineering: isolation, characterization and differentiation studies
title_full_unstemmed Bovine adipose tissue derived stem cells bioengineering: isolation, characterization and differentiation studies
title_sort bovine adipose tissue derived stem cells bioengineering: isolation, characterization and differentiation studies
publisher Universiti Malaysia Perlis (UniMAP)
publishDate 2014
url http://dspace.unimap.edu.my:80/dspace/handle/123456789/31295
_version_ 1643796438725951488
score 13.222552

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