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Cloning of urease operon from helicobacter pylori J99

The 2nd International Malaysia-Ireland Joint Symposium on Engineering, Science and Business 2012 (IMiEJS2012) jointly organized by Universiti Malaysia Perlis and Athlone Institute of Technology in collaboration with The Ministry of Higher Education (MOHE) Malaysia, Education Malaysia and Malaysia Po...

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Main Authors: Che Wan Sharifah Robiah, Mohamad, Uyub, Abd Manaf, Yahya, Mat Arip, Dr.
Other Authors: ymarip@usm.my
Format: Working Paper
Language:English
Published: Universiti Malaysia Perlis (UniMAP) 2013
Subjects:
Helicobacter pylori
Urease operon
pET 32 Ek/LIC
Online Access:http://dspace.unimap.edu.my/xmlui/handle/123456789/28988
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spelling my.unimap-289882013-10-21T03:21:16Z Cloning of urease operon from helicobacter pylori J99 Che Wan Sharifah Robiah, Mohamad Uyub, Abd Manaf Yahya, Mat Arip, Dr. ymarip@usm.my Helicobacter pylori Urease operon pET 32 Ek/LIC The 2nd International Malaysia-Ireland Joint Symposium on Engineering, Science and Business 2012 (IMiEJS2012) jointly organized by Universiti Malaysia Perlis and Athlone Institute of Technology in collaboration with The Ministry of Higher Education (MOHE) Malaysia, Education Malaysia and Malaysia Postgraduates Student Association Ireland (MyPSI), 18th - 19th June 2012 at Putra World Trade Center (PWTC), Kuala Lumpur, Malaysia. Helicobacter pylori is a gram-negative bacterium that colonizes the gastric mucosal in human stomach. It causes gastric ulcers and cancer by mechanisms that are not well understood. Helicobacter pylori produces enzyme that damage gastric epithelial cells, such as urease (constitute up to 10% of total bacterial protein) that neutralize the acidic medium permitting its survival in the human’s stomach. Urease (urea amidohydrolase; EC 3.5.1.5) is a nickel-dependent enzyme that catalyzes the hydrolysis of urea to form ammonia and carbon dioxide. The genes for urease enzymes are organized in a gene cluster or operon. In this preliminary work, the urease operon was amplified using polymerase chain reaction (PCR) and cloned into pET 32Ek/LIC (Invitrogen, USA) system. The aim of this study is to obtain a functional replicon of urease operon that capable of expressing the urease enzymes and introduce the replicon in animal model for pathogenesis, as well as, pathophysiological studies of Helicobacter pylori. 2013-10-21T03:21:16Z 2013-10-21T03:21:16Z 2012-06-18 Working Paper p. 907 - 910 978-967-5760-11-2 http://hdl.handle.net/123456789/28988 en Proceedings of the The 2nd International Malaysia-Ireland Joint Symposium on Engineering, Science and Business 2012 (IMiEJS2012); Universiti Malaysia Perlis (UniMAP)
institution Universiti Malaysia Perlis
building UniMAP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Perlis
content_source UniMAP Library Digital Repository
url_provider http://dspace.unimap.edu.my/
language English
topic Helicobacter pylori
Urease operon
pET 32 Ek/LIC
spellingShingle Helicobacter pylori
Urease operon
pET 32 Ek/LIC
Che Wan Sharifah Robiah, Mohamad
Uyub, Abd Manaf
Yahya, Mat Arip, Dr.
Cloning of urease operon from helicobacter pylori J99
description The 2nd International Malaysia-Ireland Joint Symposium on Engineering, Science and Business 2012 (IMiEJS2012) jointly organized by Universiti Malaysia Perlis and Athlone Institute of Technology in collaboration with The Ministry of Higher Education (MOHE) Malaysia, Education Malaysia and Malaysia Postgraduates Student Association Ireland (MyPSI), 18th - 19th June 2012 at Putra World Trade Center (PWTC), Kuala Lumpur, Malaysia.
author2 ymarip@usm.my
author_facet ymarip@usm.my
Che Wan Sharifah Robiah, Mohamad
Uyub, Abd Manaf
Yahya, Mat Arip, Dr.
format Working Paper
author Che Wan Sharifah Robiah, Mohamad
Uyub, Abd Manaf
Yahya, Mat Arip, Dr.
author_sort Che Wan Sharifah Robiah, Mohamad
title Cloning of urease operon from helicobacter pylori J99
title_short Cloning of urease operon from helicobacter pylori J99
title_full Cloning of urease operon from helicobacter pylori J99
title_fullStr Cloning of urease operon from helicobacter pylori J99
title_full_unstemmed Cloning of urease operon from helicobacter pylori J99
title_sort cloning of urease operon from helicobacter pylori j99
publisher Universiti Malaysia Perlis (UniMAP)
publishDate 2013
url http://dspace.unimap.edu.my/xmlui/handle/123456789/28988
_version_ 1643795644322676736
score 13.222552

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