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Effects of process parameters on the antioxidant activity of Phanerochaete chrysosporium bioactive phenolics

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Bibliographic Details
Main Author: Muhammad Fakhri, Zainuddin
Other Authors: Zulkarnain Mohd Idris
Format: Learning Object
Language:English
Published: Universiti Malaysia Perlis (UniMAP) 2013
Subjects:
Antioxidants
hanerochaete chrysosporium
Bioactive phenolics
Basidiomycetes
Online Access:http://dspace.unimap.edu.my/xmlui/handle/123456789/27727
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spelling my.unimap-277272013-08-27T07:48:32Z Effects of process parameters on the antioxidant activity of Phanerochaete chrysosporium bioactive phenolics Muhammad Fakhri, Zainuddin Zulkarnain Mohd Idris Antioxidants hanerochaete chrysosporium Bioactive phenolics Basidiomycetes Access is limited to UniMAP community. Oxidative damage to cells and macromolecules is considered to be the cause of several diseases such as coronary heart disease, arthritis, cataractogenesis, various neurodegenerative diseases including alzheimer’s disease, cancer and aging. Several studies have shown that the action of the natural antioxidants at the cellular and molecular level involves scavenging of free radicals. Thus, the search for newer bioactive compounds from the nations biodiversity for better health is a new thrust area in biotechnology. In this study, by using white-rot fungi, Phanerochaete chrysosporium, a submerged fermentation was carried out by using 2 % (w/v) of molasses as the carbon source. The media was suplemented with 2 % (w/v) of potassium dihydrogen phosphate (KH2PO4), 2 % (w/v) manganese sulphate (MnSO4) and 1% (w/v) of ammonium nitrate (NH4NO3) to enhance the strain growth. Six parameters including incubation days, inoculum size, inoculum age ,initial pH, speed agitation and temperature were studied. Incubation time, inoculums size, inoculums age and pH were identified as the most significant variables in antioxidant activity based on DPPH scavenging assay. The highest antioxidant activity, 75.3 % at concentration of 500 mg/L biomass extract was found at 3 days of incubation, 4 % (v/v) of inoculum size, 7 days of inoculum age, at initial pH 6 and 100 rpm and 25 ºC. This result is comparable to the commercial synthetic phenolic antioxidant, BHT ( 68% of inhibition at concentration of at 500 mg/L). 2013-08-27T07:48:32Z 2013-08-27T07:48:32Z 2012-06 Learning Object http://hdl.handle.net/123456789/27727 en Universiti Malaysia Perlis (UniMAP) School of Bioprocess Engineering
institution Universiti Malaysia Perlis
building UniMAP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Perlis
content_source UniMAP Library Digital Repository
url_provider http://dspace.unimap.edu.my/
language English
topic Antioxidants
hanerochaete chrysosporium
Bioactive phenolics
Basidiomycetes
spellingShingle Antioxidants
hanerochaete chrysosporium
Bioactive phenolics
Basidiomycetes
Muhammad Fakhri, Zainuddin
Effects of process parameters on the antioxidant activity of Phanerochaete chrysosporium bioactive phenolics
description Access is limited to UniMAP community.
author2 Zulkarnain Mohd Idris
author_facet Zulkarnain Mohd Idris
Muhammad Fakhri, Zainuddin
format Learning Object
author Muhammad Fakhri, Zainuddin
author_sort Muhammad Fakhri, Zainuddin
title Effects of process parameters on the antioxidant activity of Phanerochaete chrysosporium bioactive phenolics
title_short Effects of process parameters on the antioxidant activity of Phanerochaete chrysosporium bioactive phenolics
title_full Effects of process parameters on the antioxidant activity of Phanerochaete chrysosporium bioactive phenolics
title_fullStr Effects of process parameters on the antioxidant activity of Phanerochaete chrysosporium bioactive phenolics
title_full_unstemmed Effects of process parameters on the antioxidant activity of Phanerochaete chrysosporium bioactive phenolics
title_sort effects of process parameters on the antioxidant activity of phanerochaete chrysosporium bioactive phenolics
publisher Universiti Malaysia Perlis (UniMAP)
publishDate 2013
url http://dspace.unimap.edu.my/xmlui/handle/123456789/27727
_version_ 1643795388558213120
score 13.222552

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