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Study on extraction methods for protease production from Sterblus asper

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Main Author: Wan Ahmad Izzuddin, Wan Mahmood
Other Authors: Rozaini Abdullah
Format: Learning Object
Language:English
Published: Universiti Malaysia Perlis (UniMAP) 2013
Subjects:
Sterblus asper
Protease enzymes
Protein assay
Online Access:http://dspace.unimap.edu.my/xmlui/handle/123456789/27708
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spelling my.unimap-277082013-08-27T03:12:22Z Study on extraction methods for protease production from Sterblus asper Wan Ahmad Izzuddin, Wan Mahmood Rozaini Abdullah Sterblus asper Protease enzymes Protein assay Access is limited to UniMAP community. Streblus asper leaves were collected and used as a plant source for extracting protease enzymes. The enzyme was extracted by three methods and the activity of each extract was determined. Streblus asper leaves were extracted by using three methods which is extraction by phosphate buffer, extraction by distilled water and extraction with ammonium sulphate precipitation. Protein assay was carried out to determine the activity of enzyme for each type of the extraction method. The results showed that maximum enzyme activity (p<0.001) was obtained from Streblus asper leaves extracted with phosphate buffer solution. The enzyme activity significantly decreased (p<0.001) from 1.279 U/mL to 0.406 U/mL when Streblus asper was extracted with ammonium sulphate precipitation and decrease to 0.298 U/mL when Streblus asper was extracted with distilled water. This method was selected for optimization and three parameters have been chosen to investigate enzymatic activity by response surface methodology. An empirical quadratic model was applied to experimental data pertaining to the average enzymatic activity and equation describing the optimal conditions was obtained. As a conclusion, extraction by phosphate buffer produces most protease activity where the maximum enzyme (2.244 U/mL) was obtained in pH 7, 37.5ºC incubation temperature and 4 minutes homogenized time. 2013-08-27T03:12:22Z 2013-08-27T03:12:22Z 2012-06 Learning Object http://hdl.handle.net/123456789/27708 en Universiti Malaysia Perlis (UniMAP) School of Bioprocess Engineering
institution Universiti Malaysia Perlis
building UniMAP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Perlis
content_source UniMAP Library Digital Repository
url_provider http://dspace.unimap.edu.my/
language English
topic Sterblus asper
Protease enzymes
Protein assay
spellingShingle Sterblus asper
Protease enzymes
Protein assay
Wan Ahmad Izzuddin, Wan Mahmood
Study on extraction methods for protease production from Sterblus asper
description Access is limited to UniMAP community.
author2 Rozaini Abdullah
author_facet Rozaini Abdullah
Wan Ahmad Izzuddin, Wan Mahmood
format Learning Object
author Wan Ahmad Izzuddin, Wan Mahmood
author_sort Wan Ahmad Izzuddin, Wan Mahmood
title Study on extraction methods for protease production from Sterblus asper
title_short Study on extraction methods for protease production from Sterblus asper
title_full Study on extraction methods for protease production from Sterblus asper
title_fullStr Study on extraction methods for protease production from Sterblus asper
title_full_unstemmed Study on extraction methods for protease production from Sterblus asper
title_sort study on extraction methods for protease production from sterblus asper
publisher Universiti Malaysia Perlis (UniMAP)
publishDate 2013
url http://dspace.unimap.edu.my/xmlui/handle/123456789/27708
_version_ 1643795458425880576
score 13.222552

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