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Optimization of media compositions for lipase production from coconut dregs by solid state fermentation

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Bibliographic Details
Main Author: Nur Izzatul Akmar, Muhammad Zaini
Other Authors: Mohamad Fahrurrazi Tompang
Format: Learning Object
Language:English
Published: Universiti Malaysia Perlis (UniMAP) 2013
Subjects:
Lipase
Coconut Dregs
Aspergillus niger
Solid state fermentation (SSF)
Online Access:http://dspace.unimap.edu.my/xmlui/handle/123456789/27704
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spelling my.unimap-277042013-08-27T03:04:02Z Optimization of media compositions for lipase production from coconut dregs by solid state fermentation Nur Izzatul Akmar, Muhammad Zaini Mohamad Fahrurrazi Tompang Lipase Coconut Dregs Aspergillus niger Solid state fermentation (SSF) Access is limited to UniMAP community. Lipases are enzymes which catalyze the hydrolysis of triglycerides. In this study, the lipase was produced by solid state fermentation (SSF) using Aspergillus niger. In this work, the waste from extraction of coconut milk which is coconut dregs was used as substrate and supplemented with nutrients and olive oil during fermentation. The aim of this research is to optimize media compositions for lipase production by Response Surface Methodology (RSM), using Central Composite Design (CCD). The design was carried out to study the effects of parameters involved to obtain high production of lipase. In this study, concentration of carbon source (sucrose), nitrogen source (peptone) and inducer (olive oil) have being optimized. The maximum lipase production of 0.34912 U/mL was obtained after 96 hours by solid state fermentation at 30 °C in a media containing 1 % (w/v) sucrose, 0.2 % peptone (w/v) and 1 % (v/v) olive oil. The validation experiment was carried out to validate the model develop and the maximum lipase production was 0.35038 U/mL in optimum medium containing 1.08 % (w/v) sucrose, 0.20 % (w/v) peptone and 1.15 % (v/v) olive oil. 2013-08-27T03:04:02Z 2013-08-27T03:04:02Z 2012-06 Learning Object http://hdl.handle.net/123456789/27704 en Universiti Malaysia Perlis (UniMAP) School of Bioprocess Engineering
institution Universiti Malaysia Perlis
building UniMAP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Perlis
content_source UniMAP Library Digital Repository
url_provider http://dspace.unimap.edu.my/
language English
topic Lipase
Coconut Dregs
Aspergillus niger
Solid state fermentation (SSF)
spellingShingle Lipase
Coconut Dregs
Aspergillus niger
Solid state fermentation (SSF)
Nur Izzatul Akmar, Muhammad Zaini
Optimization of media compositions for lipase production from coconut dregs by solid state fermentation
description Access is limited to UniMAP community.
author2 Mohamad Fahrurrazi Tompang
author_facet Mohamad Fahrurrazi Tompang
Nur Izzatul Akmar, Muhammad Zaini
format Learning Object
author Nur Izzatul Akmar, Muhammad Zaini
author_sort Nur Izzatul Akmar, Muhammad Zaini
title Optimization of media compositions for lipase production from coconut dregs by solid state fermentation
title_short Optimization of media compositions for lipase production from coconut dregs by solid state fermentation
title_full Optimization of media compositions for lipase production from coconut dregs by solid state fermentation
title_fullStr Optimization of media compositions for lipase production from coconut dregs by solid state fermentation
title_full_unstemmed Optimization of media compositions for lipase production from coconut dregs by solid state fermentation
title_sort optimization of media compositions for lipase production from coconut dregs by solid state fermentation
publisher Universiti Malaysia Perlis (UniMAP)
publishDate 2013
url http://dspace.unimap.edu.my/xmlui/handle/123456789/27704
_version_ 1643795458130182144
score 13.222552

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