Cover Image

Screening and isolation cellulase producing fungi

Access is limited to UniMAP community.

Saved in:
Bibliographic Details
Main Author: Siti Hajar, Adnan
Other Authors: Saleha Shamsudin
Format: Learning Object
Language:English
Published: Universiti Malaysia Perlis (UniMAP) 2013
Subjects:
Lignocellulose
Cellulase enzyme
Fungi
Aspergillus spp
Online Access:http://dspace.unimap.edu.my/xmlui/handle/123456789/27694
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.unimap-27694
record_format dspace
spelling my.unimap-276942013-08-27T01:41:10Z Screening and isolation cellulase producing fungi Siti Hajar, Adnan Saleha Shamsudin Lignocellulose Cellulase enzyme Fungi Aspergillus spp Access is limited to UniMAP community. Three strains fungal were isolated from soil sample Aspergillus spp was the predominant genera in these soil samples.The most potent cellulase producer were selected for stdying their cellulase productivity on rice husk as cheap renewable agriculture waste by solid state fermentation. Aspergillus spp. was grown on selected media for the screening process to see the clearance zone of colony. Result showed that only the fungi type (a) is the best cellulase producer. These fungi were potential cellulose degrader and useful for enzyme production. Fermentation was carried out in flasks containing the different condition of moisture content, ph and inoculums size. These three parameters were studied to find the optimum condition for cellulase production. Optimization of three parameters, initial pH (4.5-5.5), moisture content (65% (w/v)-85% (w/v) and inoculum sizes (2% (w/v)-6% (w/v) were investigated and optimized by Response Surface Methodology (RSM) for optimal cellulase production by fungal type (a). A second order polynomial equation was fitted and the optimum condition was established. The result showed that the optimum condition for cellulase production was predicted at 75% (w/v) initial moisture (5.5) pH and 4% (w/v) inoculum size. The predicted optimal parameter was tested in the laboratory and the cellulase activity 1.55 mg/mL was recorded to be closed to the predicted value is 1.3872 mg/mL. 2013-08-27T01:41:10Z 2013-08-27T01:41:10Z 2012-05 Learning Object http://hdl.handle.net/123456789/27694 en Universiti Malaysia Perlis (UniMAP) School of Bioprocess Engineering
institution Universiti Malaysia Perlis
building UniMAP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Perlis
content_source UniMAP Library Digital Repository
url_provider http://dspace.unimap.edu.my/
language English
topic Lignocellulose
Cellulase enzyme
Fungi
Aspergillus spp
spellingShingle Lignocellulose
Cellulase enzyme
Fungi
Aspergillus spp
Siti Hajar, Adnan
Screening and isolation cellulase producing fungi
description Access is limited to UniMAP community.
author2 Saleha Shamsudin
author_facet Saleha Shamsudin
Siti Hajar, Adnan
format Learning Object
author Siti Hajar, Adnan
author_sort Siti Hajar, Adnan
title Screening and isolation cellulase producing fungi
title_short Screening and isolation cellulase producing fungi
title_full Screening and isolation cellulase producing fungi
title_fullStr Screening and isolation cellulase producing fungi
title_full_unstemmed Screening and isolation cellulase producing fungi
title_sort screening and isolation cellulase producing fungi
publisher Universiti Malaysia Perlis (UniMAP)
publishDate 2013
url http://dspace.unimap.edu.my/xmlui/handle/123456789/27694
_version_ 1643795456935854080
score 13.222552

Similar Items