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Extraction, separation and optimization of Gadis Lompat (Chloranthus Erectus) herb

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Main Author: Ooi, Hui Ling
Other Authors: Dr. Muhammad Syarhabil Ahmad
Format: Learning Object
Language:English
Published: Universiti Malaysia Perlis (UniMAP) 2013
Subjects:
Gadis Lompat herb
Chloranthus erectus
Antioxidant
Soxhlet extractions
Online Access:http://dspace.unimap.edu.my/xmlui/handle/123456789/27655
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spelling my.unimap-276552013-08-26T04:34:04Z Extraction, separation and optimization of Gadis Lompat (Chloranthus Erectus) herb Ooi, Hui Ling Dr. Muhammad Syarhabil Ahmad Gadis Lompat herb Chloranthus erectus Antioxidant Soxhlet extractions Access is limited to UniMAP community. The Soxhlet extraction method was used to optimize the extraction yield of Chloranthus erectus leaves. Soxhlet extractions were performed at five varies extraction time (60 minutes, 165 minutes, 270 minutes, 375 minutes, and 480 minutes) with two types of solvents (methanol and dichloromethane). The user defined in response surface methodology with two factors (time and types of solvent) was implemented. The analysis of variances (ANOVA) showed the extraction time was no significant while the type of solvent factor was a significant factor. The study showed that methanol extract was almost four times than dichloromethane extract. The highest yield of methanol was 15.317 % compared to the yield of dichloromethane which was just 3.430 %. DPPH free radical-scavenging found that dichloromethane solvent showed higher percentage (60.49 %) compared to the methanol extracted yield (59.51 %). Thin layer chromatography showed the extracted yield of Chloranthus erectus had good separation in the mobile phase of the methanol- acetic acid mixture (containing 99.5% (v/v) methanol and 0.5% acetic acid). The mixture was mixed with water at a ratio of 9:1 (v/v). This mobile phase was able to separate four visualized components for methanol extract and three visualized components for the dichloromethane extract. By using the same mobile phase, the HPLC showed that there were total 34 peaks which indicated 34 unknown compounds contained in the methanol extract of Chloranthus erectus .While HPLC showed that there were total 25 peaks which indicated there are 25 unknown compounds contain in the dichloromethane extract of Chloranthus erectus. 2013-08-26T04:34:04Z 2013-08-26T04:34:04Z 2012-06 Learning Object http://hdl.handle.net/123456789/27655 en Universiti Malaysia Perlis (UniMAP) School of Bioprocess Engineering
institution Universiti Malaysia Perlis
building UniMAP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Perlis
content_source UniMAP Library Digital Repository
url_provider http://dspace.unimap.edu.my/
language English
topic Gadis Lompat herb
Chloranthus erectus
Antioxidant
Soxhlet extractions
spellingShingle Gadis Lompat herb
Chloranthus erectus
Antioxidant
Soxhlet extractions
Ooi, Hui Ling
Extraction, separation and optimization of Gadis Lompat (Chloranthus Erectus) herb
description Access is limited to UniMAP community.
author2 Dr. Muhammad Syarhabil Ahmad
author_facet Dr. Muhammad Syarhabil Ahmad
Ooi, Hui Ling
format Learning Object
author Ooi, Hui Ling
author_sort Ooi, Hui Ling
title Extraction, separation and optimization of Gadis Lompat (Chloranthus Erectus) herb
title_short Extraction, separation and optimization of Gadis Lompat (Chloranthus Erectus) herb
title_full Extraction, separation and optimization of Gadis Lompat (Chloranthus Erectus) herb
title_fullStr Extraction, separation and optimization of Gadis Lompat (Chloranthus Erectus) herb
title_full_unstemmed Extraction, separation and optimization of Gadis Lompat (Chloranthus Erectus) herb
title_sort extraction, separation and optimization of gadis lompat (chloranthus erectus) herb
publisher Universiti Malaysia Perlis (UniMAP)
publishDate 2013
url http://dspace.unimap.edu.my/xmlui/handle/123456789/27655
_version_ 1643795446088335360
score 13.222552

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