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Optimization of antioxidant application in tissue culture of Harum Manis (Mangifera indica L.) for browning

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Main Author: Muhd Amirul Rasyid, Zahari
Other Authors: Dr. Zarina Zakaria
Format: Learning Object
Language:English
Published: Universiti Malaysia Perlis (UniMAP) 2013
Subjects:
Harum Manis mango
Mangifera Indica L
Plant tissue culture
Browning
Online Access:http://dspace.unimap.edu.my/xmlui/handle/123456789/27650
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spelling my.unimap-276502013-08-26T01:59:27Z Optimization of antioxidant application in tissue culture of Harum Manis (Mangifera indica L.) for browning Muhd Amirul Rasyid, Zahari Dr. Zarina Zakaria Harum Manis mango Mangifera Indica L Plant tissue culture Browning Access is limited to UniMAP community. Harum Manis mango, a variety of the mango fruit Mangifera Indica L., is said to be one of the best varities in the world. In mango, successful micropropagation through plant tissue culture has been achieved through somatic embryogenesis from nucellar tissues of immature ovules. The in vitro propagation of plantain Harum Manis mango countered with many problems and one of them is browning or blackening of tissues prior to culture due to oxidation of phenolic compound by polyphenolic oxidase enzyme present in the tissue when excised. An investigation purposely to catter this problem by using pamelo peel extract was conducted. In this study, volume of pamelo peel extracts, time of subcultures and condition of dehandling were factors identified caneffectively reduced the browning occurrence in mango tissue culture. The results for this study, 200 mL of pamelo peel extract had delayed the browning occurrence which is at 180 minutes. When the volume was high the time of browning to happen was longer. Condition dehandling method excised explants under water give the best results shows the longest time to explants turn to browning it take more 30 minutes than cutting in the air. Time of browning to occur will also take longer if there are subcultures. If not done subculture time of browning will taken shorter. The result shows the time of subculture before or after it will not give effect to the time of browning of the explants. 2013-08-26T01:59:27Z 2013-08-26T01:59:27Z 2012-06 Learning Object http://hdl.handle.net/123456789/27650 en Universiti Malaysia Perlis (UniMAP) School of Bioprocess Engineering
institution Universiti Malaysia Perlis
building UniMAP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Perlis
content_source UniMAP Library Digital Repository
url_provider http://dspace.unimap.edu.my/
language English
topic Harum Manis mango
Mangifera Indica L
Plant tissue culture
Browning
spellingShingle Harum Manis mango
Mangifera Indica L
Plant tissue culture
Browning
Muhd Amirul Rasyid, Zahari
Optimization of antioxidant application in tissue culture of Harum Manis (Mangifera indica L.) for browning
description Access is limited to UniMAP community.
author2 Dr. Zarina Zakaria
author_facet Dr. Zarina Zakaria
Muhd Amirul Rasyid, Zahari
format Learning Object
author Muhd Amirul Rasyid, Zahari
author_sort Muhd Amirul Rasyid, Zahari
title Optimization of antioxidant application in tissue culture of Harum Manis (Mangifera indica L.) for browning
title_short Optimization of antioxidant application in tissue culture of Harum Manis (Mangifera indica L.) for browning
title_full Optimization of antioxidant application in tissue culture of Harum Manis (Mangifera indica L.) for browning
title_fullStr Optimization of antioxidant application in tissue culture of Harum Manis (Mangifera indica L.) for browning
title_full_unstemmed Optimization of antioxidant application in tissue culture of Harum Manis (Mangifera indica L.) for browning
title_sort optimization of antioxidant application in tissue culture of harum manis (mangifera indica l.) for browning
publisher Universiti Malaysia Perlis (UniMAP)
publishDate 2013
url http://dspace.unimap.edu.my/xmlui/handle/123456789/27650
_version_ 1643795383611031552
score 13.222552

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