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A potentiometric indirect uric acid sensor based on ZnO nanoflakes and immobilized uricase

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Bibliographic Details
Main Authors: Syed Muhammad, Usman Ali, Dr., Zafar Hussain, Ibupoto, Muhammad, Kashif, Uda, Hashim, Prof. Dr., Willander, Magnus
Other Authors: syeal@itn.liu.se
Format: Article
Language:English
Published: MDPI 2013
Subjects:
ZnO nanoflakes (ZnO-NFs)
Potentiometric nanosensor
Uricase
Nafion® membrane
Online Access:http://dspace.unimap.edu.my/xmlui/handle/123456789/23982
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spelling my.unimap-239822013-06-24T04:47:16Z A potentiometric indirect uric acid sensor based on ZnO nanoflakes and immobilized uricase Syed Muhammad, Usman Ali, Dr. Zafar Hussain, Ibupoto Muhammad, Kashif Uda, Hashim, Prof. Dr. Willander, Magnus syeal@itn.liu.se zafar.hussain.ibupoto@liu.se kashif_bme@yahoo.com uda@unimap.edu.my magwi@itn.liu.se ZnO nanoflakes (ZnO-NFs) Potentiometric nanosensor Uricase Nafion® membrane Link to publisher's homepage at http://www.mdpi.com/ In the present work zinc oxide nanoflakes (ZnO-NF) structures with a wall thickness around 50 to 100 nm were synthesized on a gold coated glass substrate using a low temperature hydrothermal method. The enzyme uricase was electrostatically immobilized in conjunction with Nafion membrane on the surface of well oriented ZnO-NFs, resulting in a sensitive, selective, stable and reproducible uric acid sensor. The electrochemical response of the ZnO-NF-based sensor vs. a Ag/AgCl reference electrode was found to be linear over a relatively wide logarithmic concentration range (500 nM to 1.5 mM). In addition, the ZnO-NF structures demonstrate vast surface area that allow high enzyme loading which results provided a higher sensitivity. The proposed ZnO-NF array-based sensor exhibited a high sensitivity of ~66 mV/ decade in test electrolyte solutions of uric acid, with fast response time. The sensor response was unaffected by normal concentrations of common interferents such as ascorbic acid, glucose, and urea. 2013-03-07T07:51:44Z 2013-03-07T07:51:44Z 2012-03 Article Sensors, vol. 12(3), 2012, pages 2787-2797 1424-8220 http://www.mdpi.com/1424-8220/12/3/2787 http://hdl.handle.net/123456789/23982 en MDPI
institution Universiti Malaysia Perlis
building UniMAP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Perlis
content_source UniMAP Library Digital Repository
url_provider http://dspace.unimap.edu.my/
language English
topic ZnO nanoflakes (ZnO-NFs)
Potentiometric nanosensor
Uricase
Nafion® membrane
spellingShingle ZnO nanoflakes (ZnO-NFs)
Potentiometric nanosensor
Uricase
Nafion® membrane
Syed Muhammad, Usman Ali, Dr.
Zafar Hussain, Ibupoto
Muhammad, Kashif
Uda, Hashim, Prof. Dr.
Willander, Magnus
A potentiometric indirect uric acid sensor based on ZnO nanoflakes and immobilized uricase
description Link to publisher's homepage at http://www.mdpi.com/
author2 syeal@itn.liu.se
author_facet syeal@itn.liu.se
Syed Muhammad, Usman Ali, Dr.
Zafar Hussain, Ibupoto
Muhammad, Kashif
Uda, Hashim, Prof. Dr.
Willander, Magnus
format Article
author Syed Muhammad, Usman Ali, Dr.
Zafar Hussain, Ibupoto
Muhammad, Kashif
Uda, Hashim, Prof. Dr.
Willander, Magnus
author_sort Syed Muhammad, Usman Ali, Dr.
title A potentiometric indirect uric acid sensor based on ZnO nanoflakes and immobilized uricase
title_short A potentiometric indirect uric acid sensor based on ZnO nanoflakes and immobilized uricase
title_full A potentiometric indirect uric acid sensor based on ZnO nanoflakes and immobilized uricase
title_fullStr A potentiometric indirect uric acid sensor based on ZnO nanoflakes and immobilized uricase
title_full_unstemmed A potentiometric indirect uric acid sensor based on ZnO nanoflakes and immobilized uricase
title_sort potentiometric indirect uric acid sensor based on zno nanoflakes and immobilized uricase
publisher MDPI
publishDate 2013
url http://dspace.unimap.edu.my/xmlui/handle/123456789/23982
_version_ 1643794202447839232
score 13.211869

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