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Genetic dissection of sympatric populations of brown planthopper, Nilaparvata lugens (Stl), using DALP-PCR molecular markers

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Main Authors: Abdul Latif, M., Mohd Rafii, Yusop, Assoc. Prof. Dr., M. S., Mazid, Md Eaqub, Ali, Ahmed, Faheem, M. Y., Omar, Tan, Soon Guan, Prof. Dr.
Other Authors: alatif1965@yahoo.com
Format: Article
Language:English
Published: Hindawi Publishing Corporation 2013
Subjects:
Genetic
Brown planthopper
Sympatric populations
Nilaparvata lugens
DALP-PCR molecular markers
Online Access:http://dspace.unimap.edu.my/xmlui/handle/123456789/23376
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spelling my.unimap-233762013-02-06T04:21:47Z Genetic dissection of sympatric populations of brown planthopper, Nilaparvata lugens (Stl), using DALP-PCR molecular markers Abdul Latif, M. Mohd Rafii, Yusop, Assoc. Prof. Dr. M. S., Mazid Md Eaqub, Ali Ahmed, Faheem M. Y., Omar Tan, Soon Guan, Prof. Dr. alatif1965@yahoo.com eaqubali@gmail.com Genetic Brown planthopper Sympatric populations Nilaparvata lugens DALP-PCR molecular markers Link to publisher's homepage at http://www.hindawi.com/ Direct amplified length polymorphism (DALP) combines the advantages of a high-resolution fingerprint method and also characterizing the genetic polymorphisms. This molecular method was also found to be useful in brown planthopper, Nilaparvata lugens species complex for the analysis of genetic polymorphisms. A total of 11 populations of Nilaparvata spp. were collected from 6 locations from Malaysia. Two sympatric populations of brown planthopper, N. lugens, one from rice and the other from a weed grass (Leersia hexandra), were collected from each of five locations. N. bakeri was used as an out group. Three oligonucleotide primer pairs, DALP231/DALPR′5, DALP234/DALPR′5, and DALP235/DALPR′5 were applied in this study. The unweighted pair group method with arithmetic mean (UPGMA) dendrogram based on genetic distances for the 11 populations of Nilaparvata spp. revealed that populations belonging to the same species and the same host type clustered together irrespective of their geographical localities of capture. The populations of N. lugens formed into two distinct clusters, one was insects with high esterase activities usually captured from rice and the other was with low esterase activities usually captured from L. hexandra. N. bakeri, an out group, was the most isolated group. Analyses of principal components, molecular variance, and robustness also supported greatly to the findings of cluster analysis 2013-02-06T04:21:47Z 2013-02-06T04:21:47Z 2012 Article The Scientific World Journal, vol. 2012, 2012 1537-744X http://www.hindawi.com/journals/tswj/2012/586831/ http://hdl.handle.net/123456789/23376 en Hindawi Publishing Corporation
institution Universiti Malaysia Perlis
building UniMAP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Perlis
content_source UniMAP Library Digital Repository
url_provider http://dspace.unimap.edu.my/
language English
topic Genetic
Brown planthopper
Sympatric populations
Nilaparvata lugens
DALP-PCR molecular markers
spellingShingle Genetic
Brown planthopper
Sympatric populations
Nilaparvata lugens
DALP-PCR molecular markers
Abdul Latif, M.
Mohd Rafii, Yusop, Assoc. Prof. Dr.
M. S., Mazid
Md Eaqub, Ali
Ahmed, Faheem
M. Y., Omar
Tan, Soon Guan, Prof. Dr.
Genetic dissection of sympatric populations of brown planthopper, Nilaparvata lugens (Stl), using DALP-PCR molecular markers
description Link to publisher's homepage at http://www.hindawi.com/
author2 alatif1965@yahoo.com
author_facet alatif1965@yahoo.com
Abdul Latif, M.
Mohd Rafii, Yusop, Assoc. Prof. Dr.
M. S., Mazid
Md Eaqub, Ali
Ahmed, Faheem
M. Y., Omar
Tan, Soon Guan, Prof. Dr.
format Article
author Abdul Latif, M.
Mohd Rafii, Yusop, Assoc. Prof. Dr.
M. S., Mazid
Md Eaqub, Ali
Ahmed, Faheem
M. Y., Omar
Tan, Soon Guan, Prof. Dr.
author_sort Abdul Latif, M.
title Genetic dissection of sympatric populations of brown planthopper, Nilaparvata lugens (Stl), using DALP-PCR molecular markers
title_short Genetic dissection of sympatric populations of brown planthopper, Nilaparvata lugens (Stl), using DALP-PCR molecular markers
title_full Genetic dissection of sympatric populations of brown planthopper, Nilaparvata lugens (Stl), using DALP-PCR molecular markers
title_fullStr Genetic dissection of sympatric populations of brown planthopper, Nilaparvata lugens (Stl), using DALP-PCR molecular markers
title_full_unstemmed Genetic dissection of sympatric populations of brown planthopper, Nilaparvata lugens (Stl), using DALP-PCR molecular markers
title_sort genetic dissection of sympatric populations of brown planthopper, nilaparvata lugens (stl), using dalp-pcr molecular markers
publisher Hindawi Publishing Corporation
publishDate 2013
url http://dspace.unimap.edu.my/xmlui/handle/123456789/23376
_version_ 1643793976394776576
score 13.222552

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