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Photodynamic damage in liver carcinoma HepG2 cells

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Main Authors: Muhammad, Fakhar-e-Alam, Dr., Syed Muhammad, Usman Ali, S., Ali, S., Firdous, Muhammad, Atif, Dr., Zafar Hussain, Ibupoto, Willander, M., Prof., Mohammad, Kashif, Uda, Hashim, Prof. Dr.
Other Authors: Kashif_bme@yahoo.com
Format: Working Paper
Language:English
Published: Institute of Electrical and Electronics Engineers (IEEE) 2012
Subjects:
Photodynamic Therapy (PDT)
ZnOnanomaterials
HepG2 cells ( liver carcinoma)
He-Ne laser
Photodynaic damage
Online Access:http://dspace.unimap.edu.my/xmlui/handle/123456789/21324
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spelling my.unimap-213242012-10-11T04:20:46Z Photodynamic damage in liver carcinoma HepG2 cells Muhammad, Fakhar-e-Alam, Dr. Syed Muhammad, Usman Ali S., Ali S., Firdous Muhammad, Atif, Dr. Zafar Hussain, Ibupoto Willander, M., Prof. Mohammad, Kashif Uda, Hashim, Prof. Dr. Kashif_bme@yahoo.com Photodynamic Therapy (PDT) ZnOnanomaterials HepG2 cells ( liver carcinoma) He-Ne laser Photodynaic damage Link to publisher's homepage at http://ieeexplore.ieee.org/ Cirrhosis is the consequence of chronic liver disease, which is common in people of Asian countries. In this experimental approach, HepG2 cells are treated as experimental model (appropriate biological sample), extracted from the hepatocellular site of tissue. The author tried to demonstrate the comparison of biological damaging effects of zinc oxide nonmaterial’s (ZnO NMs) and carbon tetrachloride CCl4 labeled with HepG2 cells and normal liver tissue as experimental model. Laser scanning microscopy (confocal microscopy) as well as neutral red assay (NRA) has been applied for the assessment of cell toxicity which acts as milestone in the field of photodynamic therapy (PDT). In addition, photodynamic damage in HepG2 cell line was examined using a continuous wave (CW) He-Ne laser. Malignant cell lines are a good source for the optimization of different PDT parameters. We used HepG2 (liver carcinoma) as biological sample in first and last step of our conducted experiment. At the end, two different experiments were performed to analyze the photodynamic damage. For the first one, 1 ml of ALA (300 μg /ml) was added to cell suspension and incubated for 0-24 hours then irradiated with He-Ne lasers at wavelength 630 nm at different light doses 5, 10, 15, 20 mW (0-15 minutes suggested as optimal time of irradiation for each cell line) and obtained photodynamic damage of cell line was recorded by cell count. In the second experiment, 1 ml of ALA with different concentration 0-1000 μg/ml was added to cell suspension and incubated for 48 hours then irradiated with He-Ne Laser 630 nm at light dose 20 mw (15 minutes act as time of optimization for each cellular model) and complied photodynamic damage of relevant cell line was assessed by cell count. Loss in cell viability in labeled (HepG2 cells) is significantly higher for 630 nm He-Ne (CW). 2012-10-11T04:20:46Z 2012-10-11T04:20:46Z 2012-02-27 Working Paper p. 237-241 978-145771989-9 http://ieeexplore.ieee.org/xpl/articleDetails.jsp?tp=&arnumber=6179012 http://hdl.handle.net/123456789/21324 en Proceedings of the International Conference on Biomedical Engineering (ICoBE 2012) Institute of Electrical and Electronics Engineers (IEEE)
institution Universiti Malaysia Perlis
building UniMAP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Perlis
content_source UniMAP Library Digital Repository
url_provider http://dspace.unimap.edu.my/
language English
topic Photodynamic Therapy (PDT)
ZnOnanomaterials
HepG2 cells ( liver carcinoma)
He-Ne laser
Photodynaic damage
spellingShingle Photodynamic Therapy (PDT)
ZnOnanomaterials
HepG2 cells ( liver carcinoma)
He-Ne laser
Photodynaic damage
Muhammad, Fakhar-e-Alam, Dr.
Syed Muhammad, Usman Ali
S., Ali
S., Firdous
Muhammad, Atif, Dr.
Zafar Hussain, Ibupoto
Willander, M., Prof.
Mohammad, Kashif
Uda, Hashim, Prof. Dr.
Photodynamic damage in liver carcinoma HepG2 cells
description Link to publisher's homepage at http://ieeexplore.ieee.org/
author2 Kashif_bme@yahoo.com
author_facet Kashif_bme@yahoo.com
Muhammad, Fakhar-e-Alam, Dr.
Syed Muhammad, Usman Ali
S., Ali
S., Firdous
Muhammad, Atif, Dr.
Zafar Hussain, Ibupoto
Willander, M., Prof.
Mohammad, Kashif
Uda, Hashim, Prof. Dr.
format Working Paper
author Muhammad, Fakhar-e-Alam, Dr.
Syed Muhammad, Usman Ali
S., Ali
S., Firdous
Muhammad, Atif, Dr.
Zafar Hussain, Ibupoto
Willander, M., Prof.
Mohammad, Kashif
Uda, Hashim, Prof. Dr.
author_sort Muhammad, Fakhar-e-Alam, Dr.
title Photodynamic damage in liver carcinoma HepG2 cells
title_short Photodynamic damage in liver carcinoma HepG2 cells
title_full Photodynamic damage in liver carcinoma HepG2 cells
title_fullStr Photodynamic damage in liver carcinoma HepG2 cells
title_full_unstemmed Photodynamic damage in liver carcinoma HepG2 cells
title_sort photodynamic damage in liver carcinoma hepg2 cells
publisher Institute of Electrical and Electronics Engineers (IEEE)
publishDate 2012
url http://dspace.unimap.edu.my/xmlui/handle/123456789/21324
_version_ 1643793344884637696
score 13.222552

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