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Antioxidative properties of Diplazium esculentum extract by using pressurized hot water extractor: Optimization study

Pressurized hot water extraction (PHWE) was implemented in attempt to reduce the use of toxic organic solvent in extracting bioactive compounds from Diplazium esculentum. Comparison extraction methods were done and the results shows that the PHWE had higher 2,2-diphenyl-1-picrylhydrazyl (DPPH) free...

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Main Author: Nur Azirah, Jamial
Format: Thesis
Language:English
Published: Universiti Malaysia Perlis (UniMAP) 2012
Subjects:
Pressurized hot water extraction (PHWE)
Diplazium esculentum
Herbal medicines
Antioxidant activity
Online Access:http://dspace.unimap.edu.my/xmlui/handle/123456789/20814
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spelling my.unimap-208142012-09-04T03:23:01Z Antioxidative properties of Diplazium esculentum extract by using pressurized hot water extractor: Optimization study Nur Azirah, Jamial Pressurized hot water extraction (PHWE) Diplazium esculentum Herbal medicines Antioxidant activity Pressurized hot water extraction (PHWE) was implemented in attempt to reduce the use of toxic organic solvent in extracting bioactive compounds from Diplazium esculentum. Comparison extraction methods were done and the results shows that the PHWE had higher 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity (70%) and total phenolic contents (118.83f.lg catechin equivalentlmg dry samples) c,.ompared to autoclaving, boiling, soaking, sonicating and Soxhlet extraction. Soaking extraction had the highest total flavonoids contents (28.03f.lg rutin equivalentlmg dry extract). By applying Box-Behnken design of response surface methodology, the optimized condition for the best antioxidant activity of PH WE was at 17SoC, 21 minutes extraction time and SOmL water volume added to 2g of dried ground D. esculentum. The DPPH antiradical efficiency was analyzed where the D. esculentum extracts had ECso value of 1241.14f.lg/mL and time to reach steady state (TEC50) was 79.83 minutes. Thus, the extract was categorized as slow in scavenging the DPPH free radicals. Through HPLC, the identified flavonoids in the crude extracts were quercetin and myricetin. 2012-09-04T03:23:01Z 2012-09-04T03:23:01Z 2010 Thesis http://hdl.handle.net/123456789/20814 en Universiti Malaysia Perlis (UniMAP) School of Bioprocess Engineering
institution Universiti Malaysia Perlis
building UniMAP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Perlis
content_source UniMAP Library Digital Repository
url_provider http://dspace.unimap.edu.my/
language English
topic Pressurized hot water extraction (PHWE)
Diplazium esculentum
Herbal medicines
Antioxidant activity
spellingShingle Pressurized hot water extraction (PHWE)
Diplazium esculentum
Herbal medicines
Antioxidant activity
Nur Azirah, Jamial
Antioxidative properties of Diplazium esculentum extract by using pressurized hot water extractor: Optimization study
description Pressurized hot water extraction (PHWE) was implemented in attempt to reduce the use of toxic organic solvent in extracting bioactive compounds from Diplazium esculentum. Comparison extraction methods were done and the results shows that the PHWE had higher 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity (70%) and total phenolic contents (118.83f.lg catechin equivalentlmg dry samples) c,.ompared to autoclaving, boiling, soaking, sonicating and Soxhlet extraction. Soaking extraction had the highest total flavonoids contents (28.03f.lg rutin equivalentlmg dry extract). By applying Box-Behnken design of response surface methodology, the optimized condition for the best antioxidant activity of PH WE was at 17SoC, 21 minutes extraction time and SOmL water volume added to 2g of dried ground D. esculentum. The DPPH antiradical efficiency was analyzed where the D. esculentum extracts had ECso value of 1241.14f.lg/mL and time to reach steady state (TEC50) was 79.83 minutes. Thus, the extract was categorized as slow in scavenging the DPPH free radicals. Through HPLC, the identified flavonoids in the crude extracts were quercetin and myricetin.
format Thesis
author Nur Azirah, Jamial
author_facet Nur Azirah, Jamial
author_sort Nur Azirah, Jamial
title Antioxidative properties of Diplazium esculentum extract by using pressurized hot water extractor: Optimization study
title_short Antioxidative properties of Diplazium esculentum extract by using pressurized hot water extractor: Optimization study
title_full Antioxidative properties of Diplazium esculentum extract by using pressurized hot water extractor: Optimization study
title_fullStr Antioxidative properties of Diplazium esculentum extract by using pressurized hot water extractor: Optimization study
title_full_unstemmed Antioxidative properties of Diplazium esculentum extract by using pressurized hot water extractor: Optimization study
title_sort antioxidative properties of diplazium esculentum extract by using pressurized hot water extractor: optimization study
publisher Universiti Malaysia Perlis (UniMAP)
publishDate 2012
url http://dspace.unimap.edu.my/xmlui/handle/123456789/20814
_version_ 1643793192380792832
score 13.222552

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