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Cloning, Expression, and Antitumor Activity of Recombinant Protein of Curcin

Curcin, a protein isolated from the seeds of Jatropha curcas can be used as a cell-killing agent. To elaborate the purification methods and investigate the antitumor activity of the recombinant protein, the fragment encoding the mature protein of curcin was inserted into E. coli strain M15 and the...

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Main Authors: Luo, M. J., Liu, W. X., Yang, X. Y., Xu, Y., Yan, F., Huang, P., Chen, F.
Other Authors: chengfang@scu.edu.cn
Format: Article
Language:English
Published: Russian in Fiziologiya Rastenii 2011
Subjects:
Jatropha curcas
Esherichia coli
Curcin
Expression
Purification
Recombinant protein
Antitumor activity
Online Access:http://dspace.unimap.edu.my/xmlui/handle/123456789/16352
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spelling my.unimap-163522011-11-26T05:33:38Z Cloning, Expression, and Antitumor Activity of Recombinant Protein of Curcin Luo, M. J. Liu, W. X. Yang, X. Y. Xu, Y. Yan, F. Huang, P. Chen, F. chengfang@scu.edu.cn Jatropha curcas Esherichia coli Curcin Expression Purification Recombinant protein Antitumor activity Curcin, a protein isolated from the seeds of Jatropha curcas can be used as a cell-killing agent. To elaborate the purification methods and investigate the antitumor activity of the recombinant protein, the fragment encoding the mature protein of curcin was inserted into E. coli strain M15 and the recombinant strain was induced to express by the optimum inducer (0.5 mM isopropyl-β-D-thiogalactopyranoside). The recombinant protein was expressed in the form of the inclusion body and was purified by Ni-NTA affinity chromatography. The protein of interest was incubated with the tumor cells at various concentrations for different time. It was shown that the target protein could inhibit the growth of NCL-H446, SGC-7901, and S180 at a very low concentration. 2011-11-26T05:33:38Z 2011-11-26T05:33:38Z 2007 Article Vol. 54, No. 2, pp. 202–206. 1021-4437 http://hdl.handle.net/123456789/16352 10.1134/S1021443707020070 en Russian Journal of Plant Physiology Russian in Fiziologiya Rastenii
institution Universiti Malaysia Perlis
building UniMAP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Perlis
content_source UniMAP Library Digital Repository
url_provider http://dspace.unimap.edu.my/
language English
topic Jatropha curcas
Esherichia coli
Curcin
Expression
Purification
Recombinant protein
Antitumor activity
spellingShingle Jatropha curcas
Esherichia coli
Curcin
Expression
Purification
Recombinant protein
Antitumor activity
Luo, M. J.
Liu, W. X.
Yang, X. Y.
Xu, Y.
Yan, F.
Huang, P.
Chen, F.
Cloning, Expression, and Antitumor Activity of Recombinant Protein of Curcin
description Curcin, a protein isolated from the seeds of Jatropha curcas can be used as a cell-killing agent. To elaborate the purification methods and investigate the antitumor activity of the recombinant protein, the fragment encoding the mature protein of curcin was inserted into E. coli strain M15 and the recombinant strain was induced to express by the optimum inducer (0.5 mM isopropyl-β-D-thiogalactopyranoside). The recombinant protein was expressed in the form of the inclusion body and was purified by Ni-NTA affinity chromatography. The protein of interest was incubated with the tumor cells at various concentrations for different time. It was shown that the target protein could inhibit the growth of NCL-H446, SGC-7901, and S180 at a very low concentration.
author2 chengfang@scu.edu.cn
author_facet chengfang@scu.edu.cn
Luo, M. J.
Liu, W. X.
Yang, X. Y.
Xu, Y.
Yan, F.
Huang, P.
Chen, F.
format Article
author Luo, M. J.
Liu, W. X.
Yang, X. Y.
Xu, Y.
Yan, F.
Huang, P.
Chen, F.
author_sort Luo, M. J.
title Cloning, Expression, and Antitumor Activity of Recombinant Protein of Curcin
title_short Cloning, Expression, and Antitumor Activity of Recombinant Protein of Curcin
title_full Cloning, Expression, and Antitumor Activity of Recombinant Protein of Curcin
title_fullStr Cloning, Expression, and Antitumor Activity of Recombinant Protein of Curcin
title_full_unstemmed Cloning, Expression, and Antitumor Activity of Recombinant Protein of Curcin
title_sort cloning, expression, and antitumor activity of recombinant protein of curcin
publisher Russian in Fiziologiya Rastenii
publishDate 2011
url http://dspace.unimap.edu.my/xmlui/handle/123456789/16352
_version_ 1643791773701505024
score 13.211869

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