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Binding characteristics study for dengue virus non-structural protein 1 of antigen and its antibody by using circular dichroism technique

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Main Authors: N. A., Yusoff, N., Soin, N., Abd Rahman, R. N. Z., Raja Abd Rahman, M. K. B., Abd Kahar
Other Authors: aizas_05@yahoo.com
Format: Working Paper
Language:English
Published: Institute of Electrical and Electronic Engineers (IEEE) 2011
Subjects:
Antibodies
Chiral structures
Circular dichroism (CD)
Dengue virus
Non-structural proteins 1 (NS1)
Online Access:http://dspace.unimap.edu.my/xmlui/handle/123456789/12178
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spelling my.unimap-121782011-06-05T02:54:44Z Binding characteristics study for dengue virus non-structural protein 1 of antigen and its antibody by using circular dichroism technique N. A., Yusoff N., Soin N., Abd Rahman R. N. Z., Raja Abd Rahman M. K. B., Abd Kahar aizas_05@yahoo.com Antibodies Chiral structures Circular dichroism (CD) Dengue virus Non-structural proteins 1 (NS1) Link to publisher's homepage at http://ieeexplore.ieee.org/ This paper presents the binding characteristics study of dengue non-structural protein 1 (NS1) antigen and its antibody using circular dichroism technique in far UV region. Circular dichroism (CD) is a spectroscopic technique which measures differences in the absorption of left-handed and right handed circularly polarized light. The CD spectrum can determine conformation of the NS1 antigen and its antibody, conformational changes of the antigen-antibody interaction and estimates the secondary structure of these proteins in far UV region. Firstly, CD spectrum of individual solutions of the antigen and the antibody were measured. Then, the solutions were mixed to produce a solution of complex dengue NS1 antigen and its antibody for measurement. The findings show that the antibody has the highest positive band of CD intensity follow by the complex antigen-antibody and antigen. The antibody is a chiral structure, has high helical conformation and more ordered epitope structure. Meanwhile, the NS1 antigen shows the negative and the lowest CD spectrum. The antigen is low chirality and has more random-like conformation. The complex (binding of the antigen and antibody) has the CD spectrum's shape similar to the antibody but in lower intensity. So, it has helical and beta conformations lower than the antibody. The binding characteristics of the complex solutions were also studied with increased in incubation time and with varied rotation applied. It is found that the immunoreactions between the antigen and its antibody are rapid processes which do not require too long incubation time. Besides, the applied rotation can increased the immunoreaction process. 2011-06-05T02:54:43Z 2011-06-05T02:54:43Z 2009-12-14 Working Paper p.1-6 978-1-4244-5223-1 http://ieeexplore.ieee.org/xpls/abs_all.jsp?arnumber=5412060 http://hdl.handle.net/123456789/12178 en Proceedings of the International Conference for Technical Postgraduates (TECHPOS) 2009 Institute of Electrical and Electronic Engineers (IEEE)
institution Universiti Malaysia Perlis
building UniMAP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Perlis
content_source UniMAP Library Digital Repository
url_provider http://dspace.unimap.edu.my/
language English
topic Antibodies
Chiral structures
Circular dichroism (CD)
Dengue virus
Non-structural proteins 1 (NS1)
spellingShingle Antibodies
Chiral structures
Circular dichroism (CD)
Dengue virus
Non-structural proteins 1 (NS1)
N. A., Yusoff
N., Soin
N., Abd Rahman
R. N. Z., Raja Abd Rahman
M. K. B., Abd Kahar
Binding characteristics study for dengue virus non-structural protein 1 of antigen and its antibody by using circular dichroism technique
description Link to publisher's homepage at http://ieeexplore.ieee.org/
author2 aizas_05@yahoo.com
author_facet aizas_05@yahoo.com
N. A., Yusoff
N., Soin
N., Abd Rahman
R. N. Z., Raja Abd Rahman
M. K. B., Abd Kahar
format Working Paper
author N. A., Yusoff
N., Soin
N., Abd Rahman
R. N. Z., Raja Abd Rahman
M. K. B., Abd Kahar
author_sort N. A., Yusoff
title Binding characteristics study for dengue virus non-structural protein 1 of antigen and its antibody by using circular dichroism technique
title_short Binding characteristics study for dengue virus non-structural protein 1 of antigen and its antibody by using circular dichroism technique
title_full Binding characteristics study for dengue virus non-structural protein 1 of antigen and its antibody by using circular dichroism technique
title_fullStr Binding characteristics study for dengue virus non-structural protein 1 of antigen and its antibody by using circular dichroism technique
title_full_unstemmed Binding characteristics study for dengue virus non-structural protein 1 of antigen and its antibody by using circular dichroism technique
title_sort binding characteristics study for dengue virus non-structural protein 1 of antigen and its antibody by using circular dichroism technique
publisher Institute of Electrical and Electronic Engineers (IEEE)
publishDate 2011
url http://dspace.unimap.edu.my/xmlui/handle/123456789/12178
_version_ 1643789184634191872
score 13.222552

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