Staff View: The initial kinetics of NH3/NH4+ efflux from L3 Teladorsagia circumcincta

The initial kinetics of NH3/NH4+ efflux from L3 Teladorsagia circumcincta

Abstract The initial rate of NH3/NH4+ accumulation in a medium containing L3Teladorsagia circumcincta was 0.18–0.6 pmol h− 1 larva− 1, which increased linearly with larval density. However it appeared that the larva-generated external concentration of NH3/NH4+ did not exceed about 130 μM. The rat...

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Main Authors:	Noorzaid Muhamad, Lisa R. Walker, Kevin C. Pedley, David C. Simcock, Simon Brown
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Published:	Elsevier 2013
Subjects:	Teladorsagia circumcincta Parasite Ammonia Diffusion Kinetics
Online Access:	http://www.sciencedirect.com/science/article/pii/S1383576912000499 http://ir.unikl.edu.my/jspui/handle/123456789/2283
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spelling	my.unikl.ir-22832013-05-30T09:19:22Z The initial kinetics of NH3/NH4+ efflux from L3 Teladorsagia circumcincta Noorzaid Muhamad Lisa R. Walker Kevin C. Pedley David C. Simcock Simon Brown Teladorsagia circumcincta Parasite Ammonia Diffusion Kinetics Abstract The initial rate of NH3/NH4+ accumulation in a medium containing L3Teladorsagia circumcincta was 0.18–0.6 pmol h− 1 larva− 1, which increased linearly with larval density. However it appeared that the larva-generated external concentration of NH3/NH4+ did not exceed about 130 μM. The rate of NH3/NH4+ accumulation increased with temperature between 4 °C and 37 °C, declined with increasing pH or increasing external NH3/NH4+ concentration and was not significantly affected by the concentration of the phosphate buffer or by exsheathing the larvae. We infer from these data that the efflux of NH3/NH4+ is a diffusive process and that the secreted or excreted NH3/NH4+ is generated enzymatically rather than dissociating from the surface of the nematode. The enzymatic source of the NH3/NH4+ is yet to be identified. Since the concentration of NH3/NH4+ in the rumen and abomasum is higher than 130 μM, it is unlikely that T. circumcincta contributes to it, but NH3/NH4+ may be accumulated from the rumen fluid by the nematode. 2013-05-30T09:19:22Z 2013-05-30T09:19:22Z 2012 Parasitology International 61 (2012) 487–492 http://www.sciencedirect.com/science/article/pii/S1383576912000499 http://ir.unikl.edu.my/jspui/handle/123456789/2283 Parasitology International; Elsevier
institution	Universiti Kuala Lumpur
building	UniKL Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Kuala Lumpur
content_source	UniKL Institutional Repository
url_provider	http://ir.unikl.edu.my/
topic	Teladorsagia circumcincta Parasite Ammonia Diffusion Kinetics
spellingShingle	Teladorsagia circumcincta Parasite Ammonia Diffusion Kinetics Noorzaid Muhamad Lisa R. Walker Kevin C. Pedley David C. Simcock Simon Brown The initial kinetics of NH3/NH4+ efflux from L3 Teladorsagia circumcincta
description	Abstract The initial rate of NH3/NH4+ accumulation in a medium containing L3Teladorsagia circumcincta was 0.18–0.6 pmol h− 1 larva− 1, which increased linearly with larval density. However it appeared that the larva-generated external concentration of NH3/NH4+ did not exceed about 130 μM. The rate of NH3/NH4+ accumulation increased with temperature between 4 °C and 37 °C, declined with increasing pH or increasing external NH3/NH4+ concentration and was not significantly affected by the concentration of the phosphate buffer or by exsheathing the larvae. We infer from these data that the efflux of NH3/NH4+ is a diffusive process and that the secreted or excreted NH3/NH4+ is generated enzymatically rather than dissociating from the surface of the nematode. The enzymatic source of the NH3/NH4+ is yet to be identified. Since the concentration of NH3/NH4+ in the rumen and abomasum is higher than 130 μM, it is unlikely that T. circumcincta contributes to it, but NH3/NH4+ may be accumulated from the rumen fluid by the nematode.
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author	Noorzaid Muhamad Lisa R. Walker Kevin C. Pedley David C. Simcock Simon Brown
author_facet	Noorzaid Muhamad Lisa R. Walker Kevin C. Pedley David C. Simcock Simon Brown
author_sort	Noorzaid Muhamad
title	The initial kinetics of NH3/NH4+ efflux from L3 Teladorsagia circumcincta
title_short	The initial kinetics of NH3/NH4+ efflux from L3 Teladorsagia circumcincta
title_full	The initial kinetics of NH3/NH4+ efflux from L3 Teladorsagia circumcincta
title_fullStr	The initial kinetics of NH3/NH4+ efflux from L3 Teladorsagia circumcincta
title_full_unstemmed	The initial kinetics of NH3/NH4+ efflux from L3 Teladorsagia circumcincta
title_sort	initial kinetics of nh3/nh4+ efflux from l3 teladorsagia circumcincta
publisher	Elsevier
publishDate	2013
url	http://www.sciencedirect.com/science/article/pii/S1383576912000499 http://ir.unikl.edu.my/jspui/handle/123456789/2283
_version_	1644484647620444160
score	13.226497

The initial kinetics of NH3/NH4+ efflux from L3 Teladorsagia circumcincta

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