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OPTIMIZATION OF PROTEASE FROM ASPERGILLUS NIGER IN SOLID STATE FERMENTATION FOR ENZYMATIC TREATMENT OF AQUACULTURE SLUDGE

Proteases or proteolytic enzymes, has high potential to be applied in wastewater treatment and improve sludge dewatering. Hence, the ultimate aim of this study is to produce and characterize protease from Aspergillus niger under solid-state fermentation (SSF) by utilizing shrimp shell powder as s...

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Main Author: OOI CHEE KUAN
Format: Thesis
Language:English
Published: UNIVERSITI MALAYSIA TERENGGANU 2022
Online Access:http://umt-ir.umt.edu.my:8080/handle/123456789/16179
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spelling my.umt.ir-161792022-02-07T03:39:20Z OPTIMIZATION OF PROTEASE FROM ASPERGILLUS NIGER IN SOLID STATE FERMENTATION FOR ENZYMATIC TREATMENT OF AQUACULTURE SLUDGE OOI CHEE KUAN Proteases or proteolytic enzymes, has high potential to be applied in wastewater treatment and improve sludge dewatering. Hence, the ultimate aim of this study is to produce and characterize protease from Aspergillus niger under solid-state fermentation (SSF) by utilizing shrimp shell powder as solid substrate. The produced protease was applied for enzymatic treatment to reduce organic matter and micropollutants in aquaculture sludge based on selected parameters. It was found that the produced protease from SSF was slightly alkaline. The correlation between factors operating parameters (incubation temperature, inoculum size, moisture content) for the production of enzyme are analysed using statistical software, Minitab 16. A 23 full factorial experimental design was employed and the enzyme produced was optimized by the method of desirability function. The optimal conditions for protease production of 3.7 U/mg were 35 °C of incubation temperature, 60% of initial moisture content and 1.0 of inoculum size. It was found that aquaculture sludge collected contained high total suspended solids (TSS) and chemical oxygen demand (COD), which were 8280 mg/L and 5866.67 mg/L respectively. SSF protease and commercial protease was applied for enzymatic treatment of this aquaculture sludge by batch test for seven days. 2022-02-07T03:38:31Z 2022-02-07T03:38:31Z 2021-02 Thesis http://umt-ir.umt.edu.my:8080/handle/123456789/16179 en application/pdf application/pdf UNIVERSITI MALAYSIA TERENGGANU
institution Universiti Malaysia Terengganu
building Perpustakaan Sultanah Nur Zahirah
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Terengganu
content_source UMT-IR
url_provider http://umt-ir.umt.edu.my:8080/
language English
description Proteases or proteolytic enzymes, has high potential to be applied in wastewater treatment and improve sludge dewatering. Hence, the ultimate aim of this study is to produce and characterize protease from Aspergillus niger under solid-state fermentation (SSF) by utilizing shrimp shell powder as solid substrate. The produced protease was applied for enzymatic treatment to reduce organic matter and micropollutants in aquaculture sludge based on selected parameters. It was found that the produced protease from SSF was slightly alkaline. The correlation between factors operating parameters (incubation temperature, inoculum size, moisture content) for the production of enzyme are analysed using statistical software, Minitab 16. A 23 full factorial experimental design was employed and the enzyme produced was optimized by the method of desirability function. The optimal conditions for protease production of 3.7 U/mg were 35 °C of incubation temperature, 60% of initial moisture content and 1.0 of inoculum size. It was found that aquaculture sludge collected contained high total suspended solids (TSS) and chemical oxygen demand (COD), which were 8280 mg/L and 5866.67 mg/L respectively. SSF protease and commercial protease was applied for enzymatic treatment of this aquaculture sludge by batch test for seven days.
format Thesis
author OOI CHEE KUAN
spellingShingle OOI CHEE KUAN
OPTIMIZATION OF PROTEASE FROM ASPERGILLUS NIGER IN SOLID STATE FERMENTATION FOR ENZYMATIC TREATMENT OF AQUACULTURE SLUDGE
author_facet OOI CHEE KUAN
author_sort OOI CHEE KUAN
title OPTIMIZATION OF PROTEASE FROM ASPERGILLUS NIGER IN SOLID STATE FERMENTATION FOR ENZYMATIC TREATMENT OF AQUACULTURE SLUDGE
title_short OPTIMIZATION OF PROTEASE FROM ASPERGILLUS NIGER IN SOLID STATE FERMENTATION FOR ENZYMATIC TREATMENT OF AQUACULTURE SLUDGE
title_full OPTIMIZATION OF PROTEASE FROM ASPERGILLUS NIGER IN SOLID STATE FERMENTATION FOR ENZYMATIC TREATMENT OF AQUACULTURE SLUDGE
title_fullStr OPTIMIZATION OF PROTEASE FROM ASPERGILLUS NIGER IN SOLID STATE FERMENTATION FOR ENZYMATIC TREATMENT OF AQUACULTURE SLUDGE
title_full_unstemmed OPTIMIZATION OF PROTEASE FROM ASPERGILLUS NIGER IN SOLID STATE FERMENTATION FOR ENZYMATIC TREATMENT OF AQUACULTURE SLUDGE
title_sort optimization of protease from aspergillus niger in solid state fermentation for enzymatic treatment of aquaculture sludge
publisher UNIVERSITI MALAYSIA TERENGGANU
publishDate 2022
url http://umt-ir.umt.edu.my:8080/handle/123456789/16179
_version_ 1738395569448026112
score 13.235796

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