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OPTIMIZATION OF PROTEASE FROM APERGILLUS NIGER IN SOLID STATE FERMENTATION FOR ENZYMATIC TREATMENT OF AQUACULTURE SLUDGE

Proteases or proteolytic enzymes, has high potential to be applied in wastewater treatment and improve sludge dewatering. Hence, the ultimate aim of this study is to produce and characterize protease from Aspergillus niger under solid-state fermentation (SSF) by utilizing shrimp shell powder as soli...

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Main Author: OOI CHEE KUAN
Format: Thesis
Language:English
Published: UNIVERSITI MALAYSIA TERENGGANU 2022
Online Access:http://umt-ir.umt.edu.my:8080/handle/123456789/16121
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id my.umt.ir-16121
record_format eprints
spelling my.umt.ir-161212022-01-30T07:52:45Z OPTIMIZATION OF PROTEASE FROM APERGILLUS NIGER IN SOLID STATE FERMENTATION FOR ENZYMATIC TREATMENT OF AQUACULTURE SLUDGE OOI CHEE KUAN Proteases or proteolytic enzymes, has high potential to be applied in wastewater treatment and improve sludge dewatering. Hence, the ultimate aim of this study is to produce and characterize protease from Aspergillus niger under solid-state fermentation (SSF) by utilizing shrimp shell powder as solid substrate. The produced protease was applied for enzymatic treatment to reduce organic matter and micropollutants in aquaculture sludge based on selected parameters. It was found that the produced protease from SSF was slightly alkaline. The correlation between factors operating parameters (incubation temperature, inoculum size, moisture content) for the production of enzyme are analysed using statistical software, Minitab 16. A 23 full factorial experimental design was employed and the enzyme produced was optimized by the method of desirability function. The optimal conditions for protease production of 3.7 U/mg were 35 °C of incubation temperature, 60% of initial moisture content and 1.0 of inoculum size. 2022-01-30T07:52:27Z 2022-01-30T07:52:27Z 2021-06 Thesis http://umt-ir.umt.edu.my:8080/handle/123456789/16121 en application/pdf application/pdf UNIVERSITI MALAYSIA TERENGGANU
institution Universiti Malaysia Terengganu
building Perpustakaan Sultanah Nur Zahirah
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Terengganu
content_source UMT-IR
url_provider http://umt-ir.umt.edu.my:8080/
language English
description Proteases or proteolytic enzymes, has high potential to be applied in wastewater treatment and improve sludge dewatering. Hence, the ultimate aim of this study is to produce and characterize protease from Aspergillus niger under solid-state fermentation (SSF) by utilizing shrimp shell powder as solid substrate. The produced protease was applied for enzymatic treatment to reduce organic matter and micropollutants in aquaculture sludge based on selected parameters. It was found that the produced protease from SSF was slightly alkaline. The correlation between factors operating parameters (incubation temperature, inoculum size, moisture content) for the production of enzyme are analysed using statistical software, Minitab 16. A 23 full factorial experimental design was employed and the enzyme produced was optimized by the method of desirability function. The optimal conditions for protease production of 3.7 U/mg were 35 °C of incubation temperature, 60% of initial moisture content and 1.0 of inoculum size.
format Thesis
author OOI CHEE KUAN
spellingShingle OOI CHEE KUAN
OPTIMIZATION OF PROTEASE FROM APERGILLUS NIGER IN SOLID STATE FERMENTATION FOR ENZYMATIC TREATMENT OF AQUACULTURE SLUDGE
author_facet OOI CHEE KUAN
author_sort OOI CHEE KUAN
title OPTIMIZATION OF PROTEASE FROM APERGILLUS NIGER IN SOLID STATE FERMENTATION FOR ENZYMATIC TREATMENT OF AQUACULTURE SLUDGE
title_short OPTIMIZATION OF PROTEASE FROM APERGILLUS NIGER IN SOLID STATE FERMENTATION FOR ENZYMATIC TREATMENT OF AQUACULTURE SLUDGE
title_full OPTIMIZATION OF PROTEASE FROM APERGILLUS NIGER IN SOLID STATE FERMENTATION FOR ENZYMATIC TREATMENT OF AQUACULTURE SLUDGE
title_fullStr OPTIMIZATION OF PROTEASE FROM APERGILLUS NIGER IN SOLID STATE FERMENTATION FOR ENZYMATIC TREATMENT OF AQUACULTURE SLUDGE
title_full_unstemmed OPTIMIZATION OF PROTEASE FROM APERGILLUS NIGER IN SOLID STATE FERMENTATION FOR ENZYMATIC TREATMENT OF AQUACULTURE SLUDGE
title_sort optimization of protease from apergillus niger in solid state fermentation for enzymatic treatment of aquaculture sludge
publisher UNIVERSITI MALAYSIA TERENGGANU
publishDate 2022
url http://umt-ir.umt.edu.my:8080/handle/123456789/16121
_version_ 1738395408598564864
score 13.235796

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