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Effect of different extenders and cryoprotectants on sperm viability of mud crab, scylla tranquebarica for short term storage

The aim of the present study was to determine the extenders such as calcium-free saline, Ringer’s solution and phosphate buffer and to determine the effect of different concentrations of cryoprotectants with different duration of exposure for short-term storage of mud crab, Scylla tranquebarica sper...

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Main Authors: Siti Nor Fatihah, Nur Hazwani Dahon, Cheng-Ann Chen
Format: Article
Language:English
Published: ResearchGate 2024
Subjects:
QL614-639.8 Fishes
SB320-353.5 Vegetables
Online Access:https://eprints.ums.edu.my/id/eprint/42778/1/FULL%20TEXT.pdf
https://eprints.ums.edu.my/id/eprint/42778/
http://dx.doi.org/10.19027/jai.23.2.168-175
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spelling my.ums.eprints.427782025-02-06T05:51:28Z https://eprints.ums.edu.my/id/eprint/42778/ Effect of different extenders and cryoprotectants on sperm viability of mud crab, scylla tranquebarica for short term storage Siti Nor Fatihah Nur Hazwani Dahon Cheng-Ann Chen QL614-639.8 Fishes SB320-353.5 Vegetables The aim of the present study was to determine the extenders such as calcium-free saline, Ringer’s solution and phosphate buffer and to determine the effect of different concentrations of cryoprotectants with different duration of exposure for short-term storage of mud crab, Scylla tranquebarica sperm cells. A total of 30 mud crabs were used in the present study. In the present study, three treatments with three replicates were tested to determine the suitable extender. Three treatments (dimethyl sulfoxide, glycine and glycerol) were tested to determine the suitable cryoprotectants. Eight treatments (25, 20, 16, 4, 2, -4, -20, and -80°C) were assessed to determine the optimal cooling rate. The result showed the phosphate buffer solution produced the highest sperm viability (82.36 ± 5.84%) after 60 min compared the calcium-free saline and Ringer’s solution. Using 5% glycine as the optimal cryoprotectant, sperm viability at -80°C exhibited robust initial viability 95.17 ± 0.92% at 6 hours, 94.11 ± 0.64% at 12 hours, and 93.31 ± 0.51% at 24 hours. However, sperm viability gradually declined to 90.84 ± 2.05% by day 7, 82.82 ± 0.41% by day 14, and continued to decrease by day 21. In a nutshell, the phosphate buffer and 5% glycine proved to be the most effective extender and cryoprotectant in this study ResearchGate 2024 Article NonPeerReviewed text en https://eprints.ums.edu.my/id/eprint/42778/1/FULL%20TEXT.pdf Siti Nor Fatihah and Nur Hazwani Dahon and Cheng-Ann Chen (2024) Effect of different extenders and cryoprotectants on sperm viability of mud crab, scylla tranquebarica for short term storage. Jurnal Akuakultur Indonesia, 23. pp. 1-9. http://dx.doi.org/10.19027/jai.23.2.168-175
institution Universiti Malaysia Sabah
building UMS Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sabah
content_source UMS Institutional Repository
url_provider http://eprints.ums.edu.my/
language English
topic QL614-639.8 Fishes
SB320-353.5 Vegetables
spellingShingle QL614-639.8 Fishes
SB320-353.5 Vegetables
Siti Nor Fatihah
Nur Hazwani Dahon
Cheng-Ann Chen
Effect of different extenders and cryoprotectants on sperm viability of mud crab, scylla tranquebarica for short term storage
description The aim of the present study was to determine the extenders such as calcium-free saline, Ringer’s solution and phosphate buffer and to determine the effect of different concentrations of cryoprotectants with different duration of exposure for short-term storage of mud crab, Scylla tranquebarica sperm cells. A total of 30 mud crabs were used in the present study. In the present study, three treatments with three replicates were tested to determine the suitable extender. Three treatments (dimethyl sulfoxide, glycine and glycerol) were tested to determine the suitable cryoprotectants. Eight treatments (25, 20, 16, 4, 2, -4, -20, and -80°C) were assessed to determine the optimal cooling rate. The result showed the phosphate buffer solution produced the highest sperm viability (82.36 ± 5.84%) after 60 min compared the calcium-free saline and Ringer’s solution. Using 5% glycine as the optimal cryoprotectant, sperm viability at -80°C exhibited robust initial viability 95.17 ± 0.92% at 6 hours, 94.11 ± 0.64% at 12 hours, and 93.31 ± 0.51% at 24 hours. However, sperm viability gradually declined to 90.84 ± 2.05% by day 7, 82.82 ± 0.41% by day 14, and continued to decrease by day 21. In a nutshell, the phosphate buffer and 5% glycine proved to be the most effective extender and cryoprotectant in this study
format Article
author Siti Nor Fatihah
Nur Hazwani Dahon
Cheng-Ann Chen
author_facet Siti Nor Fatihah
Nur Hazwani Dahon
Cheng-Ann Chen
author_sort Siti Nor Fatihah
title Effect of different extenders and cryoprotectants on sperm viability of mud crab, scylla tranquebarica for short term storage
title_short Effect of different extenders and cryoprotectants on sperm viability of mud crab, scylla tranquebarica for short term storage
title_full Effect of different extenders and cryoprotectants on sperm viability of mud crab, scylla tranquebarica for short term storage
title_fullStr Effect of different extenders and cryoprotectants on sperm viability of mud crab, scylla tranquebarica for short term storage
title_full_unstemmed Effect of different extenders and cryoprotectants on sperm viability of mud crab, scylla tranquebarica for short term storage
title_sort effect of different extenders and cryoprotectants on sperm viability of mud crab, scylla tranquebarica for short term storage
publisher ResearchGate
publishDate 2024
url https://eprints.ums.edu.my/id/eprint/42778/1/FULL%20TEXT.pdf
https://eprints.ums.edu.my/id/eprint/42778/
http://dx.doi.org/10.19027/jai.23.2.168-175
_version_ 1823533991651704832
score 13.239859

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