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Establishment of an in vitro mass propagation system for dendrocalamus asper

Dendrocalamus asper is a species of bamboo that has high commercial value and is the bamboo of choice for large scale agro-forestry plantations in the tropical regions of the world. Micropropagation using tissue culture is essential to generate uniform clones that are amenable to establishment in in...

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Main Authors: Anis Adilah Mustafa, Thau, Wilson Lym Yong, Julius Kulip, Kenneth Francis Rodrigues
Format: Article
Language:English
Published: Universiti Malaysia Sabah 2023
Subjects:
QK1-989 Botany
SB1-1110 Plant culture
Online Access:https://eprints.ums.edu.my/id/eprint/42731/1/FULL%20TEXT.pdf
https://eprints.ums.edu.my/id/eprint/42731/
https://doi.org/10.51200/jtbc.v20i.4644
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spelling my.ums.eprints.427312025-01-23T03:35:18Z https://eprints.ums.edu.my/id/eprint/42731/ Establishment of an in vitro mass propagation system for dendrocalamus asper Anis Adilah Mustafa Thau, Wilson Lym Yong Julius Kulip Kenneth Francis Rodrigues QK1-989 Botany SB1-1110 Plant culture Dendrocalamus asper is a species of bamboo that has high commercial value and is the bamboo of choice for large scale agro-forestry plantations in the tropical regions of the world. Micropropagation using tissue culture is essential to generate uniform clones that are amenable to establishment in industrial agro-forestry projects for bamboo biomass, habitat restoration or in carbon sequestration. This paper reports on the establishment of D. asper invitro using commercially available seeds. The seeds were surface sterilized using three different chemical agents which were Sodium Hypochlorite (20%), Mercuric Chloride (0.1%) and Ethanol (70%) followed by shoot initiation on Murashige and Skoog (MS) medium supplemented with 6- Benzylaminopurine (BAP) with a concentration ranging from 1.0 - 5.0 mg/L. Propagules were multiplied on MS media supplemented with different concentration of IBA Indole-3-Butyric Acid (IBA) and Naphthalene Acetic Acid (NAA), and finally rooted and hardened in peat moss. The findings of our study indicate that the sterilization protocol eliminated all the plant pathogens, resulting in an axenic culture. Full strength MS medium supplemented with 5 mg/L BAP yielded the highest number of shoots (11.46 per explant) after four weeks of inoculation. The highest multiplication rate (3.95 shoots per explant) was obtained on MS medium supplemented with 3 mg/L BAP. The time required from initiation to hardening was 70 to 90 days, following which the plantlets were ready for field trials. The findings of this study will facilitate the establishment of plant tissue culture programmes dedicated to the production of D. asper locally, thus eliminating the need for imports and the possible entry of plant pathogens that can be detrimental to the local agroforestry industry. Universiti Malaysia Sabah 2023 Article NonPeerReviewed text en https://eprints.ums.edu.my/id/eprint/42731/1/FULL%20TEXT.pdf Anis Adilah Mustafa and Thau, Wilson Lym Yong and Julius Kulip and Kenneth Francis Rodrigues (2023) Establishment of an in vitro mass propagation system for dendrocalamus asper. Journal Of Tropical Biology And Conservation, 20. pp. 193-202. ISSN 1823-3920 https://doi.org/10.51200/jtbc.v20i.4644
institution Universiti Malaysia Sabah
building UMS Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sabah
content_source UMS Institutional Repository
url_provider http://eprints.ums.edu.my/
language English
topic QK1-989 Botany
SB1-1110 Plant culture
spellingShingle QK1-989 Botany
SB1-1110 Plant culture
Anis Adilah Mustafa
Thau, Wilson Lym Yong
Julius Kulip
Kenneth Francis Rodrigues
Establishment of an in vitro mass propagation system for dendrocalamus asper
description Dendrocalamus asper is a species of bamboo that has high commercial value and is the bamboo of choice for large scale agro-forestry plantations in the tropical regions of the world. Micropropagation using tissue culture is essential to generate uniform clones that are amenable to establishment in industrial agro-forestry projects for bamboo biomass, habitat restoration or in carbon sequestration. This paper reports on the establishment of D. asper invitro using commercially available seeds. The seeds were surface sterilized using three different chemical agents which were Sodium Hypochlorite (20%), Mercuric Chloride (0.1%) and Ethanol (70%) followed by shoot initiation on Murashige and Skoog (MS) medium supplemented with 6- Benzylaminopurine (BAP) with a concentration ranging from 1.0 - 5.0 mg/L. Propagules were multiplied on MS media supplemented with different concentration of IBA Indole-3-Butyric Acid (IBA) and Naphthalene Acetic Acid (NAA), and finally rooted and hardened in peat moss. The findings of our study indicate that the sterilization protocol eliminated all the plant pathogens, resulting in an axenic culture. Full strength MS medium supplemented with 5 mg/L BAP yielded the highest number of shoots (11.46 per explant) after four weeks of inoculation. The highest multiplication rate (3.95 shoots per explant) was obtained on MS medium supplemented with 3 mg/L BAP. The time required from initiation to hardening was 70 to 90 days, following which the plantlets were ready for field trials. The findings of this study will facilitate the establishment of plant tissue culture programmes dedicated to the production of D. asper locally, thus eliminating the need for imports and the possible entry of plant pathogens that can be detrimental to the local agroforestry industry.
format Article
author Anis Adilah Mustafa
Thau, Wilson Lym Yong
Julius Kulip
Kenneth Francis Rodrigues
author_facet Anis Adilah Mustafa
Thau, Wilson Lym Yong
Julius Kulip
Kenneth Francis Rodrigues
author_sort Anis Adilah Mustafa
title Establishment of an in vitro mass propagation system for dendrocalamus asper
title_short Establishment of an in vitro mass propagation system for dendrocalamus asper
title_full Establishment of an in vitro mass propagation system for dendrocalamus asper
title_fullStr Establishment of an in vitro mass propagation system for dendrocalamus asper
title_full_unstemmed Establishment of an in vitro mass propagation system for dendrocalamus asper
title_sort establishment of an in vitro mass propagation system for dendrocalamus asper
publisher Universiti Malaysia Sabah
publishDate 2023
url https://eprints.ums.edu.my/id/eprint/42731/1/FULL%20TEXT.pdf
https://eprints.ums.edu.my/id/eprint/42731/
https://doi.org/10.51200/jtbc.v20i.4644
_version_ 1823092351681167360
score 13.244413

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