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Fractionation of tilapia by-product protein hydrolysate using multilayer configuration of ultrafiltration membrane

Production of small-sized peptides is significant because of their health benefits. Ultrafiltration (UF) membrane provides an effective fractionation of small-sized peptides on a large scale. Thus, the present study was aimed to evaluate the performance of multilayer UF membrane in fractionating til...

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Main Authors: Jumardi Roslan, Siti Mazlina Mustapa Kamal, Khairul Faezah Md. Yunos, Norhafizah Abdullah
Format: Article
Language:English
Published: MDPI AG 2021
Subjects:
QL614-639.8 Fishes
TX341-641 Nutrition. Foods and food supply
Online Access:https://eprints.ums.edu.my/id/eprint/42451/1/FULL%20TEXT.pdf
https://eprints.ums.edu.my/id/eprint/42451/
https://doi.org/10.3390/pr9030446
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spelling my.ums.eprints.424512024-12-31T01:18:17Z https://eprints.ums.edu.my/id/eprint/42451/ Fractionation of tilapia by-product protein hydrolysate using multilayer configuration of ultrafiltration membrane Jumardi Roslan Siti Mazlina Mustapa Kamal Khairul Faezah Md. Yunos Norhafizah Abdullah QL614-639.8 Fishes TX341-641 Nutrition. Foods and food supply Production of small-sized peptides is significant because of their health benefits. Ultrafiltration (UF) membrane provides an effective fractionation of small-sized peptides on a large scale. Thus, the present study was aimed to evaluate the performance of multilayer UF membrane in fractionating tilapia fish by-product (TB) protein hydrolysate by observing the permeate flux, peptide transmission, and peptide distribution under different stirring speed, pH of feed solution, and salt concentration (NaCl). The fractionation process was carried out using a dead-end UF membrane system that consists of a stack of two membrane sheets with different (10/5 kDa) and similar (5/5 kDa) pore sizes in one device. The highest permeate flux (10/5 kDa–39.5 to 47.3 L/m2 .h; 5/5 kDa–15.8 to 20.3 L/m2 .h) and peptide transmission (10/5 kDa–51.8 to 61.0%; 5/5 kDa–18.3 to 23.3%) for both multilayer membrane configurations were obtained at 3.0 bar, 600 rpm, pH 8, and without the addition of salt. It was also found that the permeates were enriched with small-size peptides (<500 Da) with a concentration of 0.58 g/L (10/5 kDa) and 0.65 g/L (5/5 kDa) as compared to large-sized peptides (500–1500 Da) with concentration of 0.56 g/L (10/5 kDa) and 0.36 g/L (5/5 kDa). This might indicate the enrichment of small-size peptides through the multilayer membrane which could potentially enhance the biological activity of the protein hydrolysate fraction. MDPI AG 2021 Article NonPeerReviewed text en https://eprints.ums.edu.my/id/eprint/42451/1/FULL%20TEXT.pdf Jumardi Roslan and Siti Mazlina Mustapa Kamal and Khairul Faezah Md. Yunos and Norhafizah Abdullah (2021) Fractionation of tilapia by-product protein hydrolysate using multilayer configuration of ultrafiltration membrane. Processes, 9. pp. 1-15. https://doi.org/10.3390/pr9030446
institution Universiti Malaysia Sabah
building UMS Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sabah
content_source UMS Institutional Repository
url_provider http://eprints.ums.edu.my/
language English
topic QL614-639.8 Fishes
TX341-641 Nutrition. Foods and food supply
spellingShingle QL614-639.8 Fishes
TX341-641 Nutrition. Foods and food supply
Jumardi Roslan
Siti Mazlina Mustapa Kamal
Khairul Faezah Md. Yunos
Norhafizah Abdullah
Fractionation of tilapia by-product protein hydrolysate using multilayer configuration of ultrafiltration membrane
description Production of small-sized peptides is significant because of their health benefits. Ultrafiltration (UF) membrane provides an effective fractionation of small-sized peptides on a large scale. Thus, the present study was aimed to evaluate the performance of multilayer UF membrane in fractionating tilapia fish by-product (TB) protein hydrolysate by observing the permeate flux, peptide transmission, and peptide distribution under different stirring speed, pH of feed solution, and salt concentration (NaCl). The fractionation process was carried out using a dead-end UF membrane system that consists of a stack of two membrane sheets with different (10/5 kDa) and similar (5/5 kDa) pore sizes in one device. The highest permeate flux (10/5 kDa–39.5 to 47.3 L/m2 .h; 5/5 kDa–15.8 to 20.3 L/m2 .h) and peptide transmission (10/5 kDa–51.8 to 61.0%; 5/5 kDa–18.3 to 23.3%) for both multilayer membrane configurations were obtained at 3.0 bar, 600 rpm, pH 8, and without the addition of salt. It was also found that the permeates were enriched with small-size peptides (<500 Da) with a concentration of 0.58 g/L (10/5 kDa) and 0.65 g/L (5/5 kDa) as compared to large-sized peptides (500–1500 Da) with concentration of 0.56 g/L (10/5 kDa) and 0.36 g/L (5/5 kDa). This might indicate the enrichment of small-size peptides through the multilayer membrane which could potentially enhance the biological activity of the protein hydrolysate fraction.
format Article
author Jumardi Roslan
Siti Mazlina Mustapa Kamal
Khairul Faezah Md. Yunos
Norhafizah Abdullah
author_facet Jumardi Roslan
Siti Mazlina Mustapa Kamal
Khairul Faezah Md. Yunos
Norhafizah Abdullah
author_sort Jumardi Roslan
title Fractionation of tilapia by-product protein hydrolysate using multilayer configuration of ultrafiltration membrane
title_short Fractionation of tilapia by-product protein hydrolysate using multilayer configuration of ultrafiltration membrane
title_full Fractionation of tilapia by-product protein hydrolysate using multilayer configuration of ultrafiltration membrane
title_fullStr Fractionation of tilapia by-product protein hydrolysate using multilayer configuration of ultrafiltration membrane
title_full_unstemmed Fractionation of tilapia by-product protein hydrolysate using multilayer configuration of ultrafiltration membrane
title_sort fractionation of tilapia by-product protein hydrolysate using multilayer configuration of ultrafiltration membrane
publisher MDPI AG
publishDate 2021
url https://eprints.ums.edu.my/id/eprint/42451/1/FULL%20TEXT.pdf
https://eprints.ums.edu.my/id/eprint/42451/
https://doi.org/10.3390/pr9030446
_version_ 1821003219137986560
score 13.244413

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