Staff View: Shoot development through modified transverse Thin Cell Layer (tTCL) culture of phalaenopsis hybrid protocorms

Shoot development through modified transverse Thin Cell Layer (tTCL) culture of phalaenopsis hybrid protocorms

This first-attempt study used microtome-based methods to generate a thin cell layer culture for the micropropagation of Phal. Hwafeng Redjewel × Phal. New Cinderella. Protocorms were embedded in various agarose concentrations (8–12%, w/v) and dried from 1 to 8 h before sectioning with a microtome. O...

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Main Authors:	Lo, Kuo-Chin, Jualang Azlan Gansau, Shih, Chia-Hung, Kao, Chien-Yuan
Format:	Article
Language:	English
Published:	MDPI 2022
Subjects:	QK710-899 Plant physiology SB403-450.87 Flowers and flower culture. Ornamental plants
Online Access:	https://eprints.ums.edu.my/id/eprint/42389/1/FULL%20TEXT.pdf https://eprints.ums.edu.my/id/eprint/42389/ https://doi.org/10.3390/horticulturae8030206
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id	my.ums.eprints.42389
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spelling	my.ums.eprints.423892024-12-26T03:14:54Z https://eprints.ums.edu.my/id/eprint/42389/ Shoot development through modified transverse Thin Cell Layer (tTCL) culture of phalaenopsis hybrid protocorms Lo, Kuo-Chin Jualang Azlan Gansau Shih, Chia-Hung Kao, Chien-Yuan QK710-899 Plant physiology SB403-450.87 Flowers and flower culture. Ornamental plants This first-attempt study used microtome-based methods to generate a thin cell layer culture for the micropropagation of Phal. Hwafeng Redjewel × Phal. New Cinderella. Protocorms were embedded in various agarose concentrations (8–12%, w/v) and dried from 1 to 8 h before sectioning with a microtome. Optimal conditions for slicing sections of 100 to 300 µm were achieved when the protocorms were embedded at 10% (w/v) agarose and dried for 4 h under laminar flow, and the hardness of the agarose block under these conditions reached 641.8 ± 9.5 g·cm−2 . The sectioned protocorms that were cultured on an MS medium supplemented with 1.2 mg·L −1 6-benzylaminopurine and 0.1 mg·L −1 α-naphthaleneacetic acid were capable of growth and differentiated through the neoformation of protocorm-like bodies (PLBs) and/or callus before subsequent regeneration into plantlets and development into healthy plants in a nursery environment. The results of this study demonstrate that microtome-based tTCL is a reliable and promising approach for mass propagation and possible virus-free propagation objectives for Phalaenopsis. MDPI 2022 Article NonPeerReviewed text en https://eprints.ums.edu.my/id/eprint/42389/1/FULL%20TEXT.pdf Lo, Kuo-Chin and Jualang Azlan Gansau and Shih, Chia-Hung and Kao, Chien-Yuan (2022) Shoot development through modified transverse Thin Cell Layer (tTCL) culture of phalaenopsis hybrid protocorms. Horticulturae, 8. pp. 1-10. https://doi.org/10.3390/horticulturae8030206
institution	Universiti Malaysia Sabah
building	UMS Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Malaysia Sabah
content_source	UMS Institutional Repository
url_provider	http://eprints.ums.edu.my/
language	English
topic	QK710-899 Plant physiology SB403-450.87 Flowers and flower culture. Ornamental plants
spellingShingle	QK710-899 Plant physiology SB403-450.87 Flowers and flower culture. Ornamental plants Lo, Kuo-Chin Jualang Azlan Gansau Shih, Chia-Hung Kao, Chien-Yuan Shoot development through modified transverse Thin Cell Layer (tTCL) culture of phalaenopsis hybrid protocorms
description	This first-attempt study used microtome-based methods to generate a thin cell layer culture for the micropropagation of Phal. Hwafeng Redjewel × Phal. New Cinderella. Protocorms were embedded in various agarose concentrations (8–12%, w/v) and dried from 1 to 8 h before sectioning with a microtome. Optimal conditions for slicing sections of 100 to 300 µm were achieved when the protocorms were embedded at 10% (w/v) agarose and dried for 4 h under laminar flow, and the hardness of the agarose block under these conditions reached 641.8 ± 9.5 g·cm−2 . The sectioned protocorms that were cultured on an MS medium supplemented with 1.2 mg·L −1 6-benzylaminopurine and 0.1 mg·L −1 α-naphthaleneacetic acid were capable of growth and differentiated through the neoformation of protocorm-like bodies (PLBs) and/or callus before subsequent regeneration into plantlets and development into healthy plants in a nursery environment. The results of this study demonstrate that microtome-based tTCL is a reliable and promising approach for mass propagation and possible virus-free propagation objectives for Phalaenopsis.
format	Article
author	Lo, Kuo-Chin Jualang Azlan Gansau Shih, Chia-Hung Kao, Chien-Yuan
author_facet	Lo, Kuo-Chin Jualang Azlan Gansau Shih, Chia-Hung Kao, Chien-Yuan
author_sort	Lo, Kuo-Chin
title	Shoot development through modified transverse Thin Cell Layer (tTCL) culture of phalaenopsis hybrid protocorms
title_short	Shoot development through modified transverse Thin Cell Layer (tTCL) culture of phalaenopsis hybrid protocorms
title_full	Shoot development through modified transverse Thin Cell Layer (tTCL) culture of phalaenopsis hybrid protocorms
title_fullStr	Shoot development through modified transverse Thin Cell Layer (tTCL) culture of phalaenopsis hybrid protocorms
title_full_unstemmed	Shoot development through modified transverse Thin Cell Layer (tTCL) culture of phalaenopsis hybrid protocorms
title_sort	shoot development through modified transverse thin cell layer (ttcl) culture of phalaenopsis hybrid protocorms
publisher	MDPI
publishDate	2022
url	https://eprints.ums.edu.my/id/eprint/42389/1/FULL%20TEXT.pdf https://eprints.ums.edu.my/id/eprint/42389/ https://doi.org/10.3390/horticulturae8030206
_version_	1819911293241393152
score	13.226497

Shoot development through modified transverse Thin Cell Layer (tTCL) culture of phalaenopsis hybrid protocorms

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