Cover Image

Characterization of acid- and pepsin-soluble collagen extracted from the skin of purple-spotted bigeye snapper

Fish processing waste is a prospective source of collagen and a cost-effective environmental pollutant. The skin of the purple-spotted bigeye snapper (Priacanthus tayenus) was extracted utilising various acid soluble collagens (ASC) including acetic acid (AAC), lactic acid (LAC), citric acid (CAC) a...

Full description

Saved in:
Bibliographic Details
Main Authors: Siti Nur Hazwani Oslan, Rossita Shapawi, Ruzaidi Azli Mohd Mokhtar, Wan Norhana Md. Noordin, Nurul Huda
Format: Article
Language:English
Published: Multidisciplinary Digital Publishing Institute (MDPI) 2022
Subjects:
QL614-639.8 Fishes
TX341-641 Nutrition. Foods and food supply
Online Access:https://eprints.ums.edu.my/id/eprint/42298/2/FULL%20TEXT.pdf
https://eprints.ums.edu.my/id/eprint/42298/
https://doi.org/10.3390/gels8100665
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.ums.eprints.42298
record_format eprints
spelling my.ums.eprints.422982024-12-16T04:00:04Z https://eprints.ums.edu.my/id/eprint/42298/ Characterization of acid- and pepsin-soluble collagen extracted from the skin of purple-spotted bigeye snapper Siti Nur Hazwani Oslan Rossita Shapawi Ruzaidi Azli Mohd Mokhtar Wan Norhana Md. Noordin Nurul Huda QL614-639.8 Fishes TX341-641 Nutrition. Foods and food supply Fish processing waste is a prospective source of collagen and a cost-effective environmental pollutant. The skin of the purple-spotted bigeye snapper (Priacanthus tayenus) was extracted utilising various acid soluble collagens (ASC) including acetic acid (AAC), lactic acid (LAC), citric acid (CAC) and pepsin soluble collagens (PSC). In this study, PSC (6.65%) had the highest collagen yield, followed by AAC (5.79%), CAC (4.15%), and LAC (3.19%). The maximum temperatures (Tmax) denaturation of AAC, LAC, CAC, and PSC were 31.4, 31.7, 31.5, and 33.2 °C, respectively. UV-VIS absorption spectra showed all extracted collagens had a range of absorbance at 230 nm, due to the presence of glycine, proline, hydroxyproline, and triple-helical collagen. Additionally, they exhibited amide A, B, amide I, II, and III peaks. SDS–PAGE identified all extracted collagens as type I. The PSC had a significantly higher (p < 0.05) hydroxyproline content than acidic extraction 66.3 ± 1.03 (mg/g sample). Furthermore, all samples were extremely soluble in acetic conditions at pH 5, and all collagen was soluble in NaCl up to 3% (w/v). Therefore, PSC was the best treatment since it did not impact collagen triple helical and acetic acid yielded the most collagen in ASC extraction. Overall, the analysis revealed that fish skin waste might be used as an alternate source of collagen in diverse applications, particularly in food applications. Multidisciplinary Digital Publishing Institute (MDPI) 2022 Article NonPeerReviewed text en https://eprints.ums.edu.my/id/eprint/42298/2/FULL%20TEXT.pdf Siti Nur Hazwani Oslan and Rossita Shapawi and Ruzaidi Azli Mohd Mokhtar and Wan Norhana Md. Noordin and Nurul Huda (2022) Characterization of acid- and pepsin-soluble collagen extracted from the skin of purple-spotted bigeye snapper. Gels, 8. pp. 1-15. https://doi.org/10.3390/gels8100665
institution Universiti Malaysia Sabah
building UMS Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sabah
content_source UMS Institutional Repository
url_provider http://eprints.ums.edu.my/
language English
topic QL614-639.8 Fishes
TX341-641 Nutrition. Foods and food supply
spellingShingle QL614-639.8 Fishes
TX341-641 Nutrition. Foods and food supply
Siti Nur Hazwani Oslan
Rossita Shapawi
Ruzaidi Azli Mohd Mokhtar
Wan Norhana Md. Noordin
Nurul Huda
Characterization of acid- and pepsin-soluble collagen extracted from the skin of purple-spotted bigeye snapper
description Fish processing waste is a prospective source of collagen and a cost-effective environmental pollutant. The skin of the purple-spotted bigeye snapper (Priacanthus tayenus) was extracted utilising various acid soluble collagens (ASC) including acetic acid (AAC), lactic acid (LAC), citric acid (CAC) and pepsin soluble collagens (PSC). In this study, PSC (6.65%) had the highest collagen yield, followed by AAC (5.79%), CAC (4.15%), and LAC (3.19%). The maximum temperatures (Tmax) denaturation of AAC, LAC, CAC, and PSC were 31.4, 31.7, 31.5, and 33.2 °C, respectively. UV-VIS absorption spectra showed all extracted collagens had a range of absorbance at 230 nm, due to the presence of glycine, proline, hydroxyproline, and triple-helical collagen. Additionally, they exhibited amide A, B, amide I, II, and III peaks. SDS–PAGE identified all extracted collagens as type I. The PSC had a significantly higher (p < 0.05) hydroxyproline content than acidic extraction 66.3 ± 1.03 (mg/g sample). Furthermore, all samples were extremely soluble in acetic conditions at pH 5, and all collagen was soluble in NaCl up to 3% (w/v). Therefore, PSC was the best treatment since it did not impact collagen triple helical and acetic acid yielded the most collagen in ASC extraction. Overall, the analysis revealed that fish skin waste might be used as an alternate source of collagen in diverse applications, particularly in food applications.
format Article
author Siti Nur Hazwani Oslan
Rossita Shapawi
Ruzaidi Azli Mohd Mokhtar
Wan Norhana Md. Noordin
Nurul Huda
author_facet Siti Nur Hazwani Oslan
Rossita Shapawi
Ruzaidi Azli Mohd Mokhtar
Wan Norhana Md. Noordin
Nurul Huda
author_sort Siti Nur Hazwani Oslan
title Characterization of acid- and pepsin-soluble collagen extracted from the skin of purple-spotted bigeye snapper
title_short Characterization of acid- and pepsin-soluble collagen extracted from the skin of purple-spotted bigeye snapper
title_full Characterization of acid- and pepsin-soluble collagen extracted from the skin of purple-spotted bigeye snapper
title_fullStr Characterization of acid- and pepsin-soluble collagen extracted from the skin of purple-spotted bigeye snapper
title_full_unstemmed Characterization of acid- and pepsin-soluble collagen extracted from the skin of purple-spotted bigeye snapper
title_sort characterization of acid- and pepsin-soluble collagen extracted from the skin of purple-spotted bigeye snapper
publisher Multidisciplinary Digital Publishing Institute (MDPI)
publishDate 2022
url https://eprints.ums.edu.my/id/eprint/42298/2/FULL%20TEXT.pdf
https://eprints.ums.edu.my/id/eprint/42298/
https://doi.org/10.3390/gels8100665
_version_ 1818835203024486400
score 13.223943

Similar Items