Staff View: Kelulut honey improves folliculogenesis, steroidogenic, and aromatase enzyme profiles and ovarian histomorphology in letrozole-induced polycystic ovary syndrome rats

Kelulut honey improves folliculogenesis, steroidogenic, and aromatase enzyme profiles and ovarian histomorphology in letrozole-induced polycystic ovary syndrome rats

Polycystic ovary syndrome (PCOS) has been linked to aberrant folliculogenesis and abnormalities in the aromatase enzyme (Cyp19a1) and the steroidogenic enzyme, 17-alpha-hydroxylase (Cyp17a1) expression. It has been demonstrated that Kelulut honey (KH) improves both female and male reproductive syste...

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Main Authors:	Datu Agasi Mohd Kamal, Siti Fatimah Ibrahim, Azizah Ugusman, Siti Sarah Mohamad Zaid, Mohd Helmy Mokhtar
Format:	Article
Language:	English
Published:	MDPI 2022
Subjects:	QL360-599.82 Invertebrates RC31-1245 Internal medicine
Online Access:	https://eprints.ums.edu.my/id/eprint/42296/2/FULL%20TEXT.pdf https://eprints.ums.edu.my/id/eprint/42296/ https://doi.org/10.3390/nu14204364
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spelling	my.ums.eprints.422962024-12-16T03:59:04Z https://eprints.ums.edu.my/id/eprint/42296/ Kelulut honey improves folliculogenesis, steroidogenic, and aromatase enzyme profiles and ovarian histomorphology in letrozole-induced polycystic ovary syndrome rats Datu Agasi Mohd Kamal Siti Fatimah Ibrahim Azizah Ugusman Siti Sarah Mohamad Zaid Mohd Helmy Mokhtar QL360-599.82 Invertebrates RC31-1245 Internal medicine Polycystic ovary syndrome (PCOS) has been linked to aberrant folliculogenesis and abnormalities in the aromatase enzyme (Cyp19a1) and the steroidogenic enzyme, 17-alpha-hydroxylase (Cyp17a1) expression. It has been demonstrated that Kelulut honey (KH) improves both female and male reproductive system anomalies in animal studies. Here, we examined the effects of isolated and combined KH, metformin, and clomiphene in improving folliculogenesis, aromatase, and steroidogenic enzyme profiles and ovarian histomorphology in letrozole-induced PCOS rats. Letrozole (1 mg/kg/day) was administered to female Sprague–Dawley (SD) rats for 21 days to induce PCOS. PCOS rats were subsequently divided into six experimental groups: untreated, treatment with metformin (500 mg/kg/day), clomiphene (2 mg/kg/day), KH (1 g/kg/day), combined KH (1 g/kg/day) and metformin (500 mg/kg/day), and combined KH (1 g/kg/day) and clomiphene (2 mg/kg/day). All treatments were given orally for 35 days. We found that KH was comparable with clomiphene and metformin in improving the expression of Cyp17a1 and Cyp19a1, apart from enhancing folliculogenesis both histologically and through the expression of folliculogenesis-related genes. Besides, the combination of KH with clomiphene was the most effective treatment in improving the ovarian histomorphology of PCOS rats. The effectiveness of KH in restoring altered folliculogenesis, steroidogenic, and aromatase enzyme profiles in PCOS warrants a future clinical trial to validate its therapeutic effect clinically MDPI 2022 Article NonPeerReviewed text en https://eprints.ums.edu.my/id/eprint/42296/2/FULL%20TEXT.pdf Datu Agasi Mohd Kamal and Siti Fatimah Ibrahim and Azizah Ugusman and Siti Sarah Mohamad Zaid and Mohd Helmy Mokhtar (2022) Kelulut honey improves folliculogenesis, steroidogenic, and aromatase enzyme profiles and ovarian histomorphology in letrozole-induced polycystic ovary syndrome rats. Nutrients, 14. pp. 1-17. https://doi.org/10.3390/nu14204364
institution	Universiti Malaysia Sabah
building	UMS Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Malaysia Sabah
content_source	UMS Institutional Repository
url_provider	http://eprints.ums.edu.my/
language	English
topic	QL360-599.82 Invertebrates RC31-1245 Internal medicine
spellingShingle	QL360-599.82 Invertebrates RC31-1245 Internal medicine Datu Agasi Mohd Kamal Siti Fatimah Ibrahim Azizah Ugusman Siti Sarah Mohamad Zaid Mohd Helmy Mokhtar Kelulut honey improves folliculogenesis, steroidogenic, and aromatase enzyme profiles and ovarian histomorphology in letrozole-induced polycystic ovary syndrome rats
description	Polycystic ovary syndrome (PCOS) has been linked to aberrant folliculogenesis and abnormalities in the aromatase enzyme (Cyp19a1) and the steroidogenic enzyme, 17-alpha-hydroxylase (Cyp17a1) expression. It has been demonstrated that Kelulut honey (KH) improves both female and male reproductive system anomalies in animal studies. Here, we examined the effects of isolated and combined KH, metformin, and clomiphene in improving folliculogenesis, aromatase, and steroidogenic enzyme profiles and ovarian histomorphology in letrozole-induced PCOS rats. Letrozole (1 mg/kg/day) was administered to female Sprague–Dawley (SD) rats for 21 days to induce PCOS. PCOS rats were subsequently divided into six experimental groups: untreated, treatment with metformin (500 mg/kg/day), clomiphene (2 mg/kg/day), KH (1 g/kg/day), combined KH (1 g/kg/day) and metformin (500 mg/kg/day), and combined KH (1 g/kg/day) and clomiphene (2 mg/kg/day). All treatments were given orally for 35 days. We found that KH was comparable with clomiphene and metformin in improving the expression of Cyp17a1 and Cyp19a1, apart from enhancing folliculogenesis both histologically and through the expression of folliculogenesis-related genes. Besides, the combination of KH with clomiphene was the most effective treatment in improving the ovarian histomorphology of PCOS rats. The effectiveness of KH in restoring altered folliculogenesis, steroidogenic, and aromatase enzyme profiles in PCOS warrants a future clinical trial to validate its therapeutic effect clinically
format	Article
author	Datu Agasi Mohd Kamal Siti Fatimah Ibrahim Azizah Ugusman Siti Sarah Mohamad Zaid Mohd Helmy Mokhtar
author_facet	Datu Agasi Mohd Kamal Siti Fatimah Ibrahim Azizah Ugusman Siti Sarah Mohamad Zaid Mohd Helmy Mokhtar
author_sort	Datu Agasi Mohd Kamal
title	Kelulut honey improves folliculogenesis, steroidogenic, and aromatase enzyme profiles and ovarian histomorphology in letrozole-induced polycystic ovary syndrome rats
title_short	Kelulut honey improves folliculogenesis, steroidogenic, and aromatase enzyme profiles and ovarian histomorphology in letrozole-induced polycystic ovary syndrome rats
title_full	Kelulut honey improves folliculogenesis, steroidogenic, and aromatase enzyme profiles and ovarian histomorphology in letrozole-induced polycystic ovary syndrome rats
title_fullStr	Kelulut honey improves folliculogenesis, steroidogenic, and aromatase enzyme profiles and ovarian histomorphology in letrozole-induced polycystic ovary syndrome rats
title_full_unstemmed	Kelulut honey improves folliculogenesis, steroidogenic, and aromatase enzyme profiles and ovarian histomorphology in letrozole-induced polycystic ovary syndrome rats
title_sort	kelulut honey improves folliculogenesis, steroidogenic, and aromatase enzyme profiles and ovarian histomorphology in letrozole-induced polycystic ovary syndrome rats
publisher	MDPI
publishDate	2022
url	https://eprints.ums.edu.my/id/eprint/42296/2/FULL%20TEXT.pdf https://eprints.ums.edu.my/id/eprint/42296/ https://doi.org/10.3390/nu14204364
_version_	1818835202740322304
score	13.226497

Kelulut honey improves folliculogenesis, steroidogenic, and aromatase enzyme profiles and ovarian histomorphology in letrozole-induced polycystic ovary syndrome rats

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