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Transcriptomic signature of horseshoe crab carcinoscorpius rotundicauda hemocytes’ response to lipopolysaccharides

Carcinoscorpius rotundicauda (C. rotundicauda) is one of the four species of horseshoe crabs (HSCs). The HSC hemocytes store defense molecules that are released upon encountering invading pathogens. The HSCs rely on this innate immunity to continue its existence as a living fossil for more than 480...

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Main Authors: Maria E. Sarmiento 1 ,, Chin, Kai Ling, Lau, Nyok-Sean, Noraznawati Ismail, Mohd Nor Norazmi, Armando Acosta, Nik Soriani Yaacob
Format: Article
Language:English
English
Published: MDPI 2022
Subjects:
QL360-599.82 Invertebrates
QP501-801 Animal biochemistry
Online Access:https://eprints.ums.edu.my/id/eprint/42274/1/ABSTRACT.pdf
https://eprints.ums.edu.my/id/eprint/42274/2/FULL%20TEXT.pdf
https://eprints.ums.edu.my/id/eprint/42274/
https://doi.org/10.3390/cimb44120399
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spelling my.ums.eprints.422742024-12-16T03:27:35Z https://eprints.ums.edu.my/id/eprint/42274/ Transcriptomic signature of horseshoe crab carcinoscorpius rotundicauda hemocytes’ response to lipopolysaccharides Maria E. Sarmiento 1 , Chin, Kai Ling Lau, Nyok-Sean Noraznawati Ismail Mohd Nor Norazmi Armando Acosta Nik Soriani Yaacob QL360-599.82 Invertebrates QP501-801 Animal biochemistry Carcinoscorpius rotundicauda (C. rotundicauda) is one of the four species of horseshoe crabs (HSCs). The HSC hemocytes store defense molecules that are released upon encountering invading pathogens. The HSCs rely on this innate immunity to continue its existence as a living fossil for more than 480 million years. To gain insight into the innate mechanisms involved, transcriptomic analysis was performed on isolated C. rotundicauda hemocytes challenged with lipopolysaccharides (LPS), the main components of the outer cell membrane of gram-negative bacteria. RNA-sequencing with Illumina HiSeq platform resulted in 232,628,086 and 245,448,176 raw reads corresponding to 190,326,253 and 201,180,020 high-quality mappable reads from control and LPS-stimulated hemocytes, respectively. Following LPS-stimulation, 79 genes were significantly upregulated and 265 genes were downregulated. The differentially expressed genes (DEGs) were related to multiple immune functional categories and pathways such as those of the cytoskeleton, Toll and Imd, apoptosis, MAP kinase (MAPK), inositol phosphate metabolism, phagosome, leucocyte endothelial migration, and gram-negative bacterial infection, among others. This study provides important information about the mechanisms of response to LPS, which is relevant for the understanding the HSCs’ immune response. MDPI 2022 Article NonPeerReviewed text en https://eprints.ums.edu.my/id/eprint/42274/1/ABSTRACT.pdf text en https://eprints.ums.edu.my/id/eprint/42274/2/FULL%20TEXT.pdf Maria E. Sarmiento 1 , and Chin, Kai Ling and Lau, Nyok-Sean and Noraznawati Ismail and Mohd Nor Norazmi and Armando Acosta and Nik Soriani Yaacob (2022) Transcriptomic signature of horseshoe crab carcinoscorpius rotundicauda hemocytes’ response to lipopolysaccharides. Current Issues in Molecular Biology, 44. pp. 1-13. https://doi.org/10.3390/cimb44120399
institution Universiti Malaysia Sabah
building UMS Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sabah
content_source UMS Institutional Repository
url_provider http://eprints.ums.edu.my/
language English
English
topic QL360-599.82 Invertebrates
QP501-801 Animal biochemistry
spellingShingle QL360-599.82 Invertebrates
QP501-801 Animal biochemistry
Maria E. Sarmiento 1 ,
Chin, Kai Ling
Lau, Nyok-Sean
Noraznawati Ismail
Mohd Nor Norazmi
Armando Acosta
Nik Soriani Yaacob
Transcriptomic signature of horseshoe crab carcinoscorpius rotundicauda hemocytes’ response to lipopolysaccharides
description Carcinoscorpius rotundicauda (C. rotundicauda) is one of the four species of horseshoe crabs (HSCs). The HSC hemocytes store defense molecules that are released upon encountering invading pathogens. The HSCs rely on this innate immunity to continue its existence as a living fossil for more than 480 million years. To gain insight into the innate mechanisms involved, transcriptomic analysis was performed on isolated C. rotundicauda hemocytes challenged with lipopolysaccharides (LPS), the main components of the outer cell membrane of gram-negative bacteria. RNA-sequencing with Illumina HiSeq platform resulted in 232,628,086 and 245,448,176 raw reads corresponding to 190,326,253 and 201,180,020 high-quality mappable reads from control and LPS-stimulated hemocytes, respectively. Following LPS-stimulation, 79 genes were significantly upregulated and 265 genes were downregulated. The differentially expressed genes (DEGs) were related to multiple immune functional categories and pathways such as those of the cytoskeleton, Toll and Imd, apoptosis, MAP kinase (MAPK), inositol phosphate metabolism, phagosome, leucocyte endothelial migration, and gram-negative bacterial infection, among others. This study provides important information about the mechanisms of response to LPS, which is relevant for the understanding the HSCs’ immune response.
format Article
author Maria E. Sarmiento 1 ,
Chin, Kai Ling
Lau, Nyok-Sean
Noraznawati Ismail
Mohd Nor Norazmi
Armando Acosta
Nik Soriani Yaacob
author_facet Maria E. Sarmiento 1 ,
Chin, Kai Ling
Lau, Nyok-Sean
Noraznawati Ismail
Mohd Nor Norazmi
Armando Acosta
Nik Soriani Yaacob
author_sort Maria E. Sarmiento 1 ,
title Transcriptomic signature of horseshoe crab carcinoscorpius rotundicauda hemocytes’ response to lipopolysaccharides
title_short Transcriptomic signature of horseshoe crab carcinoscorpius rotundicauda hemocytes’ response to lipopolysaccharides
title_full Transcriptomic signature of horseshoe crab carcinoscorpius rotundicauda hemocytes’ response to lipopolysaccharides
title_fullStr Transcriptomic signature of horseshoe crab carcinoscorpius rotundicauda hemocytes’ response to lipopolysaccharides
title_full_unstemmed Transcriptomic signature of horseshoe crab carcinoscorpius rotundicauda hemocytes’ response to lipopolysaccharides
title_sort transcriptomic signature of horseshoe crab carcinoscorpius rotundicauda hemocytes’ response to lipopolysaccharides
publisher MDPI
publishDate 2022
url https://eprints.ums.edu.my/id/eprint/42274/1/ABSTRACT.pdf
https://eprints.ums.edu.my/id/eprint/42274/2/FULL%20TEXT.pdf
https://eprints.ums.edu.my/id/eprint/42274/
https://doi.org/10.3390/cimb44120399
_version_ 1818835199887147008
score 13.222552

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