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Apoptotic induction in human cancer cell lines by antimicrobial compounds from Antarctic streptomyces fildesensis (INACH3013)

The Antarctic Streptomyces fildesensis has been recognized for its production of antimicrobial compounds with interesting biological activities against foodborne bacteria and multi-resistant strains, but not for its potential antiproliferative activity and mechanisms involved. Two bioactive ethyl ac...

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Main Authors: David Astudillo-Barraza, Rómulo Oses Pedraza, Carlos Henríquez-Cas, Clemente Michael Vui Ling Wong, José M. Pérez-Donoso, Cristina Purcarea, Heidge Fukumasu, Natalia Fierro-Vásquez, Pablo A. Pérez, Paris Lavin
Format: Article
Language:English
English
Published: Multidisciplinary Digital Publishing Institute (MDPI) 2023
Subjects:
RC254-282 Neoplasms. Tumors. Oncology Including cancer and carcinogens
RM265-267 Antibiotic therapy. Antibiotics
Online Access:https://eprints.ums.edu.my/id/eprint/42253/1/ABSTRACT.pdf
https://eprints.ums.edu.my/id/eprint/42253/2/FULL%20TEXT.pdf
https://eprints.ums.edu.my/id/eprint/42253/
https://doi.org/10.3390/fermentation9020129
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spelling my.ums.eprints.422532024-12-16T03:25:24Z https://eprints.ums.edu.my/id/eprint/42253/ Apoptotic induction in human cancer cell lines by antimicrobial compounds from Antarctic streptomyces fildesensis (INACH3013) David Astudillo-Barraza Rómulo Oses Pedraza Carlos Henríquez-Cas Clemente Michael Vui Ling Wong José M. Pérez-Donoso Cristina Purcarea Heidge Fukumasu Natalia Fierro-Vásquez Pablo A. Pérez Paris Lavin RC254-282 Neoplasms. Tumors. Oncology Including cancer and carcinogens RM265-267 Antibiotic therapy. Antibiotics The Antarctic Streptomyces fildesensis has been recognized for its production of antimicrobial compounds with interesting biological activities against foodborne bacteria and multi-resistant strains, but not for its potential antiproliferative activity and mechanisms involved. Two bioactive ethyl acetate extract (EAE) fractions were purified via thin-layer chromatography and High-Performance Liquid Chromatography (HPLC), showing that orange-colored compounds displayed antimicrobial activity against pathogenic bacteria even after shock thermal treatment. The UV–VIS features of the active compounds, the TLC assay with actinomycin-D pure standard, Fourier transform infrared (FTIR) spectra and the ANTISMASH analysis support the presence of actinomycin-like compounds. We demonstrated that S. fildesensis displays antiproliferative activity against human tumor cell lines, including human breast cancer (MCF-7), prostate cancer (PC-3), colon cancer (HT-29) and non-tumoral colon epithelial cells (CoN). The half-maximal effective concentrations (EC50) ranged from 3.98 µg/mL to 0.1 µg/mL. Our results reveal that actinomycin-like compounds of S. fildesensis induced apoptosis mediated by caspase activation, decreasing the mitochondrial membrane potential and altering the cell morphology in all tumoral and non-tumoral cell lines analyzed. These findings confirm the potential of the psychrotolerant Antarctic S. fildesensis species as a promising source for obtaining potential novel anticancer compounds. Multidisciplinary Digital Publishing Institute (MDPI) 2023 Article NonPeerReviewed text en https://eprints.ums.edu.my/id/eprint/42253/1/ABSTRACT.pdf text en https://eprints.ums.edu.my/id/eprint/42253/2/FULL%20TEXT.pdf David Astudillo-Barraza and Rómulo Oses Pedraza and Carlos Henríquez-Cas and Clemente Michael Vui Ling Wong and José M. Pérez-Donoso and Cristina Purcarea and Heidge Fukumasu and Natalia Fierro-Vásquez and Pablo A. Pérez and Paris Lavin (2023) Apoptotic induction in human cancer cell lines by antimicrobial compounds from Antarctic streptomyces fildesensis (INACH3013). Fermentation, 9. pp. 1-15. https://doi.org/10.3390/fermentation9020129
institution Universiti Malaysia Sabah
building UMS Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sabah
content_source UMS Institutional Repository
url_provider http://eprints.ums.edu.my/
language English
English
topic RC254-282 Neoplasms. Tumors. Oncology Including cancer and carcinogens
RM265-267 Antibiotic therapy. Antibiotics
spellingShingle RC254-282 Neoplasms. Tumors. Oncology Including cancer and carcinogens
RM265-267 Antibiotic therapy. Antibiotics
David Astudillo-Barraza
Rómulo Oses Pedraza
Carlos Henríquez-Cas
Clemente Michael Vui Ling Wong
José M. Pérez-Donoso
Cristina Purcarea
Heidge Fukumasu
Natalia Fierro-Vásquez
Pablo A. Pérez
Paris Lavin
Apoptotic induction in human cancer cell lines by antimicrobial compounds from Antarctic streptomyces fildesensis (INACH3013)
description The Antarctic Streptomyces fildesensis has been recognized for its production of antimicrobial compounds with interesting biological activities against foodborne bacteria and multi-resistant strains, but not for its potential antiproliferative activity and mechanisms involved. Two bioactive ethyl acetate extract (EAE) fractions were purified via thin-layer chromatography and High-Performance Liquid Chromatography (HPLC), showing that orange-colored compounds displayed antimicrobial activity against pathogenic bacteria even after shock thermal treatment. The UV–VIS features of the active compounds, the TLC assay with actinomycin-D pure standard, Fourier transform infrared (FTIR) spectra and the ANTISMASH analysis support the presence of actinomycin-like compounds. We demonstrated that S. fildesensis displays antiproliferative activity against human tumor cell lines, including human breast cancer (MCF-7), prostate cancer (PC-3), colon cancer (HT-29) and non-tumoral colon epithelial cells (CoN). The half-maximal effective concentrations (EC50) ranged from 3.98 µg/mL to 0.1 µg/mL. Our results reveal that actinomycin-like compounds of S. fildesensis induced apoptosis mediated by caspase activation, decreasing the mitochondrial membrane potential and altering the cell morphology in all tumoral and non-tumoral cell lines analyzed. These findings confirm the potential of the psychrotolerant Antarctic S. fildesensis species as a promising source for obtaining potential novel anticancer compounds.
format Article
author David Astudillo-Barraza
Rómulo Oses Pedraza
Carlos Henríquez-Cas
Clemente Michael Vui Ling Wong
José M. Pérez-Donoso
Cristina Purcarea
Heidge Fukumasu
Natalia Fierro-Vásquez
Pablo A. Pérez
Paris Lavin
author_facet David Astudillo-Barraza
Rómulo Oses Pedraza
Carlos Henríquez-Cas
Clemente Michael Vui Ling Wong
José M. Pérez-Donoso
Cristina Purcarea
Heidge Fukumasu
Natalia Fierro-Vásquez
Pablo A. Pérez
Paris Lavin
author_sort David Astudillo-Barraza
title Apoptotic induction in human cancer cell lines by antimicrobial compounds from Antarctic streptomyces fildesensis (INACH3013)
title_short Apoptotic induction in human cancer cell lines by antimicrobial compounds from Antarctic streptomyces fildesensis (INACH3013)
title_full Apoptotic induction in human cancer cell lines by antimicrobial compounds from Antarctic streptomyces fildesensis (INACH3013)
title_fullStr Apoptotic induction in human cancer cell lines by antimicrobial compounds from Antarctic streptomyces fildesensis (INACH3013)
title_full_unstemmed Apoptotic induction in human cancer cell lines by antimicrobial compounds from Antarctic streptomyces fildesensis (INACH3013)
title_sort apoptotic induction in human cancer cell lines by antimicrobial compounds from antarctic streptomyces fildesensis (inach3013)
publisher Multidisciplinary Digital Publishing Institute (MDPI)
publishDate 2023
url https://eprints.ums.edu.my/id/eprint/42253/1/ABSTRACT.pdf
https://eprints.ums.edu.my/id/eprint/42253/2/FULL%20TEXT.pdf
https://eprints.ums.edu.my/id/eprint/42253/
https://doi.org/10.3390/fermentation9020129
_version_ 1818835196940648448
score 13.226497

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