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Type I collagen from the skin of barracuda (sphyraena sp.) prepared with different organic acids: biochemical, microstructural and functional properties

This study was carried out to compare the extractability and characteristics of barracuda (Sphyraena sp.) skin collagen using various organic acids. Acetic-solubilized collagen (ASBS), lacticsolubilized collagen (LSBS) and citric-solubilized collagen (CSBS) yielded 6.77 g/100 g, 10.06 g/100 g and 8....

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Main Authors: Nur Nadiah Matarsim, Abdul Aziz Jaziri, Rossita Shapawi, Ruzaidi Azli Mohd Mokhtar, Wan Norhana Md. Noordin, Nurul Huda
Format: Article
Language:English
English
Published: MDPI 2023
Subjects:
QL614-639.8 Fishes
SH334.5-334.7 Fishery technology
Online Access:https://eprints.ums.edu.my/id/eprint/42248/1/ABSTRACT.pdf
https://eprints.ums.edu.my/id/eprint/42248/2/FULL%20TEXT.pdf
https://eprints.ums.edu.my/id/eprint/42248/
https://doi.org/10.3390/jfb14020087
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spelling my.ums.eprints.422482024-12-16T03:25:01Z https://eprints.ums.edu.my/id/eprint/42248/ Type I collagen from the skin of barracuda (sphyraena sp.) prepared with different organic acids: biochemical, microstructural and functional properties Nur Nadiah Matarsim Abdul Aziz Jaziri Rossita Shapawi Ruzaidi Azli Mohd Mokhtar Wan Norhana Md. Noordin Nurul Huda QL614-639.8 Fishes SH334.5-334.7 Fishery technology This study was carried out to compare the extractability and characteristics of barracuda (Sphyraena sp.) skin collagen using various organic acids. Acetic-solubilized collagen (ASBS), lacticsolubilized collagen (LSBS) and citric-solubilized collagen (CSBS) yielded 6.77 g/100 g, 10.06 g/100 g and 8.35 g/100 g, respectively, and those yields were significantly different (p < 0.05). All acid solubilized collagens were considered as type I because of their two alpha chains (α1 and α2) detected in acrylamide gel after electrophoresis. Ultraviolet–visible (UV–vis) analysis confirmed that ASBS, LSBS and CSBS had similar absorption peaks (230.5 nm) and the results were in accordance with other fish collagens. Under infrared (IR) and X-ray diffraction (XRD) analysis, the triple helical structure of type I collagens extracted from barracuda skin was maintained. From a thermostability study, all type I collagens showed a higher maximum transition temperature (Tmax = 40.16 to 41.29 ◦C) compared to other fish skin collagens. In addition, the functional properties of the extracted collagens revealed the ASBS had higher water and oil absorption capacities than the CSBS and LSBS samples. The highest level of the emulsion ability index (EAI) (>200 m2/g) was detected under acidic conditions (pH 4), while lower EAIs were recorded under the alkaline (pH 10) and neutral treatments (pH 7). All type I collagens had a higher relative solubility (>60%) at a low pH test but the solubility level sharply decreased at a neutral pH. In addition to this, a lower concentration of NaCl (0–20 g/L) showed the higher percentage of solubility (>60%) while adding over 30 g/L of NaCl decreased solubility (>40%). From a microstructural test, all type I samples had an irregular and dense flake structure with random coiled filaments. Overall, collagen extracted from the barracuda skin may be applied as an alternative collagen from an industry perspective. MDPI 2023 Article NonPeerReviewed text en https://eprints.ums.edu.my/id/eprint/42248/1/ABSTRACT.pdf text en https://eprints.ums.edu.my/id/eprint/42248/2/FULL%20TEXT.pdf Nur Nadiah Matarsim and Abdul Aziz Jaziri and Rossita Shapawi and Ruzaidi Azli Mohd Mokhtar and Wan Norhana Md. Noordin and Nurul Huda (2023) Type I collagen from the skin of barracuda (sphyraena sp.) prepared with different organic acids: biochemical, microstructural and functional properties. Journal of Functional Biomaterials, 14. pp. 1-17. https://doi.org/10.3390/jfb14020087
institution Universiti Malaysia Sabah
building UMS Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sabah
content_source UMS Institutional Repository
url_provider http://eprints.ums.edu.my/
language English
English
topic QL614-639.8 Fishes
SH334.5-334.7 Fishery technology
spellingShingle QL614-639.8 Fishes
SH334.5-334.7 Fishery technology
Nur Nadiah Matarsim
Abdul Aziz Jaziri
Rossita Shapawi
Ruzaidi Azli Mohd Mokhtar
Wan Norhana Md. Noordin
Nurul Huda
Type I collagen from the skin of barracuda (sphyraena sp.) prepared with different organic acids: biochemical, microstructural and functional properties
description This study was carried out to compare the extractability and characteristics of barracuda (Sphyraena sp.) skin collagen using various organic acids. Acetic-solubilized collagen (ASBS), lacticsolubilized collagen (LSBS) and citric-solubilized collagen (CSBS) yielded 6.77 g/100 g, 10.06 g/100 g and 8.35 g/100 g, respectively, and those yields were significantly different (p < 0.05). All acid solubilized collagens were considered as type I because of their two alpha chains (α1 and α2) detected in acrylamide gel after electrophoresis. Ultraviolet–visible (UV–vis) analysis confirmed that ASBS, LSBS and CSBS had similar absorption peaks (230.5 nm) and the results were in accordance with other fish collagens. Under infrared (IR) and X-ray diffraction (XRD) analysis, the triple helical structure of type I collagens extracted from barracuda skin was maintained. From a thermostability study, all type I collagens showed a higher maximum transition temperature (Tmax = 40.16 to 41.29 ◦C) compared to other fish skin collagens. In addition, the functional properties of the extracted collagens revealed the ASBS had higher water and oil absorption capacities than the CSBS and LSBS samples. The highest level of the emulsion ability index (EAI) (>200 m2/g) was detected under acidic conditions (pH 4), while lower EAIs were recorded under the alkaline (pH 10) and neutral treatments (pH 7). All type I collagens had a higher relative solubility (>60%) at a low pH test but the solubility level sharply decreased at a neutral pH. In addition to this, a lower concentration of NaCl (0–20 g/L) showed the higher percentage of solubility (>60%) while adding over 30 g/L of NaCl decreased solubility (>40%). From a microstructural test, all type I samples had an irregular and dense flake structure with random coiled filaments. Overall, collagen extracted from the barracuda skin may be applied as an alternative collagen from an industry perspective.
format Article
author Nur Nadiah Matarsim
Abdul Aziz Jaziri
Rossita Shapawi
Ruzaidi Azli Mohd Mokhtar
Wan Norhana Md. Noordin
Nurul Huda
author_facet Nur Nadiah Matarsim
Abdul Aziz Jaziri
Rossita Shapawi
Ruzaidi Azli Mohd Mokhtar
Wan Norhana Md. Noordin
Nurul Huda
author_sort Nur Nadiah Matarsim
title Type I collagen from the skin of barracuda (sphyraena sp.) prepared with different organic acids: biochemical, microstructural and functional properties
title_short Type I collagen from the skin of barracuda (sphyraena sp.) prepared with different organic acids: biochemical, microstructural and functional properties
title_full Type I collagen from the skin of barracuda (sphyraena sp.) prepared with different organic acids: biochemical, microstructural and functional properties
title_fullStr Type I collagen from the skin of barracuda (sphyraena sp.) prepared with different organic acids: biochemical, microstructural and functional properties
title_full_unstemmed Type I collagen from the skin of barracuda (sphyraena sp.) prepared with different organic acids: biochemical, microstructural and functional properties
title_sort type i collagen from the skin of barracuda (sphyraena sp.) prepared with different organic acids: biochemical, microstructural and functional properties
publisher MDPI
publishDate 2023
url https://eprints.ums.edu.my/id/eprint/42248/1/ABSTRACT.pdf
https://eprints.ums.edu.my/id/eprint/42248/2/FULL%20TEXT.pdf
https://eprints.ums.edu.my/id/eprint/42248/
https://doi.org/10.3390/jfb14020087
_version_ 1818835196229713920
score 13.222552

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