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Potential of empty fruit bunch (EFB) treated with Ganoderma lucidum as feedstuff for ruminants

For the past years, there are growing interest in utilizing empty fruit bunch (EFB) as feed for ruminants. However, the potential use of EFB as ruminant feedstuff is still constrained by its high lignin content which is known to negatively correlate with the digestibility of ruminants. In the meanti...

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Main Author: Nur nazratul fareha mohd yasid
Format: Thesis
Language:English
English
Published: 2020
Subjects:
SF94.5-99 Feeds and feeding. Animal nutrition
Online Access:https://eprints.ums.edu.my/id/eprint/42057/1/24%20PAGES.pdf
https://eprints.ums.edu.my/id/eprint/42057/2/FULLTEXT.pdf
https://eprints.ums.edu.my/id/eprint/42057/
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spelling my.ums.eprints.420572024-12-16T02:45:59Z https://eprints.ums.edu.my/id/eprint/42057/ Potential of empty fruit bunch (EFB) treated with Ganoderma lucidum as feedstuff for ruminants Nur nazratul fareha mohd yasid SF94.5-99 Feeds and feeding. Animal nutrition For the past years, there are growing interest in utilizing empty fruit bunch (EFB) as feed for ruminants. However, the potential use of EFB as ruminant feedstuff is still constrained by its high lignin content which is known to negatively correlate with the digestibility of ruminants. In the meantime, biological delignification using white-rot fungi was reported to enhance the feeding values of many locally available agricultural by-products for ruminant rations. Thus, this study was conducted to i) identify the Ganoderma lucidum isolate, and determine the ligninolytic enzyme activity of the fungal mycelia culture, ii) determine the nutritive composition and surface morphology of EFB treated with mycelia culture of G. lucidum and iii) determine the in vitro gas production kinetics and digestibility of EFB treated with mycelia culture of G. lucidum. The isolate of G. lucidum has been identified molecularly and its ligninolytic enzyme activity was determined using spectrophotometer. Shredded EFB was sterilized and inoculated with mycelia culture of G. lucidum. All inoculated EFB were incubated for 2, 4, 6, 8, 10 and 12 weeks. They were analysed for nutritive composition and their surface morphology were observed under the scanning electron microscope (SEM). In vitro gas production (IVGP) of incubated EFB were determined for 2, 4, 6, 8, 10, 12, 24, 36, 48 and 72 hours of incubation time and its kinetics were fitted to the equation p=a+b(1–e-ct). The mycelia culture was identified as G. lucidum strain IUM 4303 (JQ520182.1). The enzyme activity assay showed that G. lucidum mycelia culture able to produce all the ligninolytic enzyme at 14th day of incubation where lignin peroxidase was recorded for 2.046 U mL-1, 0.012 U mL-1 for manganese peroxidase and 0.006 U mL-1 for laccase. All nutritive composition of treated EFB was changed significantly across the incubation period. Treated EFB at week 12 has the lowest fibre composition where cellulose with 22.08%, hemicellulose with 14.15% and lignin with only 7.49%. All fibre composition was degraded by 24% (cellulose), 29% (hemicellulose) and 41% (lignin) across the 12 weeks of incubation. The SEM images of treated EFB with G. lucidum showed that there was removal of silica bodies and great disruptions on the fibre surface. Treated EFB at week 12 have the highest IVGP (72.56 mL) with high a+b (73.79 mL) and c (0.025 mL hr-1) of gas production. It also has the highest IVDMD (77.2%) and IVOMD (69.78%). These results suggested that EFB have the potential to be utilized as one of the feed resources for ruminants after biological delignification using G. lucidum. 2020 Thesis NonPeerReviewed text en https://eprints.ums.edu.my/id/eprint/42057/1/24%20PAGES.pdf text en https://eprints.ums.edu.my/id/eprint/42057/2/FULLTEXT.pdf Nur nazratul fareha mohd yasid (2020) Potential of empty fruit bunch (EFB) treated with Ganoderma lucidum as feedstuff for ruminants. Masters thesis, Universiti Malaysia Sabah.
institution Universiti Malaysia Sabah
building UMS Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sabah
content_source UMS Institutional Repository
url_provider http://eprints.ums.edu.my/
language English
English
topic SF94.5-99 Feeds and feeding. Animal nutrition
spellingShingle SF94.5-99 Feeds and feeding. Animal nutrition
Nur nazratul fareha mohd yasid
Potential of empty fruit bunch (EFB) treated with Ganoderma lucidum as feedstuff for ruminants
description For the past years, there are growing interest in utilizing empty fruit bunch (EFB) as feed for ruminants. However, the potential use of EFB as ruminant feedstuff is still constrained by its high lignin content which is known to negatively correlate with the digestibility of ruminants. In the meantime, biological delignification using white-rot fungi was reported to enhance the feeding values of many locally available agricultural by-products for ruminant rations. Thus, this study was conducted to i) identify the Ganoderma lucidum isolate, and determine the ligninolytic enzyme activity of the fungal mycelia culture, ii) determine the nutritive composition and surface morphology of EFB treated with mycelia culture of G. lucidum and iii) determine the in vitro gas production kinetics and digestibility of EFB treated with mycelia culture of G. lucidum. The isolate of G. lucidum has been identified molecularly and its ligninolytic enzyme activity was determined using spectrophotometer. Shredded EFB was sterilized and inoculated with mycelia culture of G. lucidum. All inoculated EFB were incubated for 2, 4, 6, 8, 10 and 12 weeks. They were analysed for nutritive composition and their surface morphology were observed under the scanning electron microscope (SEM). In vitro gas production (IVGP) of incubated EFB were determined for 2, 4, 6, 8, 10, 12, 24, 36, 48 and 72 hours of incubation time and its kinetics were fitted to the equation p=a+b(1–e-ct). The mycelia culture was identified as G. lucidum strain IUM 4303 (JQ520182.1). The enzyme activity assay showed that G. lucidum mycelia culture able to produce all the ligninolytic enzyme at 14th day of incubation where lignin peroxidase was recorded for 2.046 U mL-1, 0.012 U mL-1 for manganese peroxidase and 0.006 U mL-1 for laccase. All nutritive composition of treated EFB was changed significantly across the incubation period. Treated EFB at week 12 has the lowest fibre composition where cellulose with 22.08%, hemicellulose with 14.15% and lignin with only 7.49%. All fibre composition was degraded by 24% (cellulose), 29% (hemicellulose) and 41% (lignin) across the 12 weeks of incubation. The SEM images of treated EFB with G. lucidum showed that there was removal of silica bodies and great disruptions on the fibre surface. Treated EFB at week 12 have the highest IVGP (72.56 mL) with high a+b (73.79 mL) and c (0.025 mL hr-1) of gas production. It also has the highest IVDMD (77.2%) and IVOMD (69.78%). These results suggested that EFB have the potential to be utilized as one of the feed resources for ruminants after biological delignification using G. lucidum.
format Thesis
author Nur nazratul fareha mohd yasid
author_facet Nur nazratul fareha mohd yasid
author_sort Nur nazratul fareha mohd yasid
title Potential of empty fruit bunch (EFB) treated with Ganoderma lucidum as feedstuff for ruminants
title_short Potential of empty fruit bunch (EFB) treated with Ganoderma lucidum as feedstuff for ruminants
title_full Potential of empty fruit bunch (EFB) treated with Ganoderma lucidum as feedstuff for ruminants
title_fullStr Potential of empty fruit bunch (EFB) treated with Ganoderma lucidum as feedstuff for ruminants
title_full_unstemmed Potential of empty fruit bunch (EFB) treated with Ganoderma lucidum as feedstuff for ruminants
title_sort potential of empty fruit bunch (efb) treated with ganoderma lucidum as feedstuff for ruminants
publishDate 2020
url https://eprints.ums.edu.my/id/eprint/42057/1/24%20PAGES.pdf
https://eprints.ums.edu.my/id/eprint/42057/2/FULLTEXT.pdf
https://eprints.ums.edu.my/id/eprint/42057/
_version_ 1818835178248732672
score 13.222552

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